jak inhibitors derived from imidazopiperidine fusion

专利摘要:
the invention provides compounds of formula (i): (i) where the variables are defined in the specification, or a pharmaceutically acceptable salt, therefore, which are useful as inhibitors of jak kinases. the invention also provides pharmaceutical compositions that include such compounds, methods of using such compounds to treat respiratory diseases and processes and intermediates useful for preparing such compounds.
公开号:BR112019018649A2
申请号:R112019018649
申请日:2018-03-08
公开日:2020-04-07
发明作者:Smith Cameron；E L Brandt Gary；D Crater Glenn；Jean Van Orden Lori；A Kleinschek Melanie；R Fatheree Paul；d e sullivan Steven
申请人:Theravance Biopharma R&D Ip Llc；
IPC主号:

专利说明:

JAK INHIBITORS DERIVED FROM IMIDAZOPIPERIDINE FUSION
HISTORY OF THE INVENTION
Field of the invention [001J The invention is directed to compounds useful as JAK kinase inhibitors. The invention is also directed to pharmaceutical compositions that include such compounds, methods of using such compounds to treat respiratory diseases and processes and intermediates useful for preparing such compounds.
Esrado da nécnica [002] Asthma is a chronic disease of the airways for which there is no prevention or cure. The disease is characterized by inflammation, fibrosis, hyperresponsiveness and airway remodeling, which contribute to the limitation of airflow. An estimated 300 million people worldwide suffer from asthma and it is estimated that the number of people with asthma will grow by more than 100 million by 2025. In the United States, asthma affects about 6% to 8% of population, making it one of the most common chronic diseases in the country. Although most patients can get control of asthma symptoms with the use of inhaled corticosteroids that can be combined with. a leukotriene modifier and / or a long-acting beta-agonist, there remains a subset of patients with severe asthma whose disease is not controlled with conventional therapies. Severe persistent asthma is defined as a disease that remains uncontrolled at high doses of inhaled corticosteroids.
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Although severe asthmatics are estimated to be about 5% of all asthma patients, they do. high risk of morbidity and mortality and are responsible for a disproportionate share of the use of health services among asthmatics. There is still a need for new therapies to treat these patients.
[003] Cytokines are intracellular signaling molecules that include chemokines, interferons, interleukins, lymphokines and tumor necrosis factor. Cytokines are critical for normal cell growth and immunoregulation, but they also trigger diseases mediated by the immune system and contribute to the growth of malignant cells. Elevated levels of various cytokines are involved in the pathology of asthma inflammation. For example, it has been shown that antibody-based therapies targeting interleukins (IL) -5 and 13 provide clinical benefit in. subsets of severe asthmatic patients. Among the cytokines involved in asthma inflammation, many act through signaling pathways dependent on the Janus family of tyrosine kinases (JAKs), which signal, via the transcription factor and transcription activator (STAT) family of transcription factors. The cytokines involved in asthma inflammation that signal via the JAK-STAT pathway include IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-11, IL-13, IL-23 , IL31, IL-27, thymic stromal polyoxyethylene (TSLP), gamma interferon (ΙΕΝγ) and stimulating factor for granulocyte-macrophage colony (GM-CSF).
[004] The JAK family consists of four members, JAK1, JAK2, JAK3 and tyrosine kinase 2 (TYK2). Binding of the cytokine to a JAK-dependent cytokine receptor induces
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3/136 receptor dimerization that results in phosphorylation of
waste t irosii I was going to JAK lase, activating to JAK. The JAKs f o s f o r i 1 a. d a s, by í turn if 1: igam and phosphoryl Lam several STA proteins , T that dimerize., ii 11 e rn a1i z am η o core of
cell and directly modulate gene transcription, leading, among other effects, to downstream effects associated with inflammatory disease. JAKs are generally associated with cytokine receptors in pairs such as homodimers and heterodimers. Specific cytokines are associated with specific JAK pairings. Each of the four members of the JAK family is involved in signaling at least one of the cytokines associated with. inflammation of asthma. Consequently, a chemical inhibitor with global activity against all members of the JAK family could modulate a wide range of pro-inflammatory pathways that contribute to severe asthma.
[005] However, the broad anti-inflammatory effect of such inhibitors can suppress the function of normal immune cells, potentially leading to an increased risk of infection. Evidence of an increased risk of infection was seen with the JAK inhibitor tofacitinib, which is administered orally for the treatment of rheumatoid arthritis. In asthma, inflammation is located in the respiratory tract. Inflammation of the airways is characteristic of respiratory diseases other than asthma. Chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), pneumonitis, interstitial lung diseases (including idiopathic pulmonary fibrosis), acute lung injury, acute respiratory distress syndrome, bronchitis, emphysema, obliterating bronchiolitis and sarcoidosis are also diseases of the
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4/136 respiratory tract in which they believe ^- that the tisiopatoloqia is related to cytokine signaling JAK. The local administration of one. Inhalation JAK inhibitor for lungs offers the potential to be therapeutically effective by providing a potent anti-cytokine agent directly at the site of action, limiting systemic exposure and therefore limiting the potential for adverse systemic immunosuppression. There remains a need for a potent JAK inhibitor suitable for local administration to the lungs to treat respiratory disease.
[0061 Cytokine JAK signaling also plays an important role in the activation of T cells, um. immune cell subtype that is central to many immune processes. The pathological activation of T cells is fundamental in the etiology of several respiratory diseases. Autoreactive T cells play a role in bronchiolitis obliterans. organizing pneumonia (also called BOOP). Similar to BOOP, the etiology of lung transplant rejections is associated with an aberrant activation of the activation of the T cells of the recipients by the donor transplanted lung. Lung transplant rejections can occur early as primary graft dysfunction (PGD), organizing pneumonia (PO), acute rejection (RA) or lymphocytic bronchiolitis (BL) or they can occur years after lung transplantation as chronic lung graft dysfunction (CLAD). CLAD was previously known as bronchiolitis obliterans (BO), but is now considered one. syndrome that can have different pathological manifestations including BO, restrictive CLAD (rCLAD or RAS) and neutrophilic graft dysfunction. THE
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Petition 870190088769, of 09/09/2019, p. 19/165 chronic pulmonary graft dysfunction (CLAD) is a major challenge for the long-term management of lung transplant recipients as it does with. that the transplanted lung gradually loses its functionality (Gauthier et. al., Curr Transplant R.ep., 2016, 3 (3), 18b— 191). CLAD is not very sensitive to treatment and, therefore, there remains a need for effective compounds capable of preventing or treating this condition. Several JAK-dependent cytokines, such as ΙΕΝγ and IL-5, are over-regulated in CLAD and in lung transplant rejection (Berastegui et al, Clin Transplant. 2017, 31, el2898). In addition, high levels of pulmonary CXCR3 chemokines such as CXCL9 and CXCL10 that are downstream of JAK-dependent IFN signaling are related to worse outcomes in patients undergoing lung transplantation (Shino et al, PLGS One, 2017, 12 (7) , e0180281). Systemic JAK inhibition has been shown to be effective in rejection of kidney transplantation (Vicenti et al., American Journal of Transplantation, 2012, 12, 2446-56). Therefore, JAK inhibitors have the potential to be effective in treating or preventing lung transplantation and CLAD. Events similar to T cell activation as described as the basis for lung transplant rejection are also considered to be the main factor of graft versus lung disease (GVHD) that can occur after hematopoietic stem cell transplantation. Similar to CLAD, pulmonary GVHD is a progressive condition with extremely poor outcomes and there is no currently approved treatment. A multicenter, retrospective research study of 95 patients with acute or chronic steroid refractory GVHD who received the inhibitor
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6/13 6 systemic JAK ruxolitinib as rescue therapy has demonstrated complete or partial response to ruxolitinib in most patients, including those with pulmonary GHD (Zeiser et al, Leukemia, 2015, 29, 10, 2062-68). As the systemic inhibition of JAK is associated with serious adverse events and a small therapeutic index, there remains a need for a non-systemic JAK inhibitor, directed to the lung to prevent / or treat lung transplant rejection or lung DECK.
SUMMARY OF THE INVENTION [007] In one aspect, the invention provides new compounds with activity as inhibitors of JAK kinase.
[008] Consequently, the invention provides a compound of formula (I):
R ¹ is selected from the
Cs-ecicloalkyl, and X is selected
CH ₂ R ¹⁶ , or hydrogen, Ci-aalkyl, and the
from -C (O) R and R ¹ is selected from - (CH ₂ ) 2NR ^2u R '~ and a 4- to 6-membered heterocycle containing a nitrogen atom, where a nitrogen atom is optionally substituted by R ^z2 and X is selected from -CH ₂ OR ² ' ³ and -C (O) OR ²⁴ ,
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7/136 where
R ^z is selected from -NR ¹³ R ¹⁴ and -OR ¹⁵ , and R ¹⁴ was combined with the nitrogen atom to which they are attached form a 6 or 7 membered monocyclic or bicyclic heterocycle containing an additional nitrogen atom, where the additional nitrogen atom is replaced by R ³ and the heterocycle is optionally replaced by one or two R ⁴ or
Rt ° and R ¹⁴ together with the nitrogen atom to which they are attached form a 5- to 6-membered heterocycle, where the heterocycle is optionally substituted by -NR ⁵ R ⁶ and R ⁷ , or
R ^1J and R ¹⁴ together with the nitrogen atom to which they are attached form morpholinyl, or
R ¹³ is R ⁸ and R ¹⁴ is R ⁹ ,
R ³ is selected from hydrogen, C ₃ _ scycloalkyl and C ₃ alkyl, where C ₃ alkyl is optionally substituted by -OH or -OC ₃ alkyl,
R ⁴ is C- ₃ alkyl, where C- ₃ alkyl is optionally substituted by -OH,
R ”and R ^b are independently C- ₃ alkyl or R“ and R ° together with the nitrogen atom to which they are attached form a 5- or 6-membered heterocycle, optionally including an oxygen atom,
· R 'is Cl - ₃ alkyl, optionally substituted by one.
5- or 6-membered heterocycle containing a nitrogen atom,
R ⁸ is hydrogen or C ₃ alkyl,
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R ^y is - (CH ₂ ) 2NR ^{± 0} R ¹¹ or a 4- to 6-membered heterocycle containing a nitrogen atom, where ο nitrogen is optionally substituted by R ~ ² ,
R ° θ * _s ~ Rn _n i d _and pendently or -C salquil ^LU R ¹¹ and R together with the nitrogen atom to which they are attached form a 5- or 6 - membered,
R ⁱ² is C1-3 alkyl or C3-6cycloalkyl, where C1-3alkyl is optionally substituted by -ΌΗ,
RC ⁵ is selected from Cl - ₃ alkyl, C3ecicloalquil and a 5- or 6 members, including one heteroatom selected from nitrogen and oxygen, CI - ₃ alkyl which is optionally substituted with -OH or -N (CI- ₃ alkyl) and _3f and a heterocycle is 5 or 6 membered optionally substituted by C ₃ alkyl,
R ⁶ is selected from - NR ~ ⁷ R ²⁸ and —OR ² ',
R * 'and R * ° are independently Ci_4alkyl or C ₃ _ scicloalkyl or R ¹ ' and R ¹⁸ together with the nitrogen atom to which they are attached form a 5- or 6-membered heterocycle, optionally including an oxygen atom, where heterocycle is optionally substituted by Cl - ₃ alkyl,
R ^iy , R ²⁰ , R ²ⁱ , R ²² , R ^z 'and R ^z4 are independently selected from Ci-salquil, or a pharmaceutically acceptable salt.
[009J As used below, the phrase compound of formula (I), means a compound of formula (I) or its pharmaceutically acceptable salt, that is, this phrase means a compound of formula (I) in the form of base
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9/13 6 free or in the form of a pharmaceutically acceptable salt, unless otherwise indicated.
[0010] The invention also provides a pharmaceutical composition that includes a compound of the invention and a. pharmaceutically acceptable carrier.
[0011] The invention also provides a method for treating inflammatory respiratory disease, in particular asthma, in a mammal, the method including administering to the mammal a compound or a pharmaceutical composition of the invention. In separate and distinct aspects, the invention will also provide synthetic and intermediate processes described herein useful for preparing the compounds of the invention.
[0012] The invention also provides a compound of the invention as described herein for use in medical therapy, as well as the use of a compound of the invention in the manufacture of a formulation or medicament to treat respiratory disease in a mammal.
DETAILED DESCRIPTION OF THE INVENTION [0013] Among other aspects, the invention provides JAK kinase inhibitors of formula (I), their pharmaceutically acceptable salts and intermediates for their preparation. The following substituents and values are intended to provide representative examples of various aspects of this invention. These representative values are intended to define such aspects and are not intended to exclude other values or to limit the scope of the invention.
[0014] In a specific aspect, R ¹ is selected from hydrogen, Ci-salquil, and C3-6cycloalkyl.il.
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10/136 [0015] In another specific aspect, R ^± is selected from hydrogen and Ci-3alkyl. In yet another aspect, R ¹ is C1-3 alkyl.
[0016] The specific values of R ¹ include., But are not limited to, methyl, ethyl, n-propyl and isopropyl.
[0017] In a specific aspect, R ¹ is selected from hydrogen and Ci-3alkyl and X is -C (O) R ² , where R ^z is NR ¹³ R ¹⁴ , where R ^1J and R ¹⁴ in. together with the nitrogen atom to which they are attached form a 6 or 7 membered monocyclic or bicyclic heterocycle containing an additional nitrogen atom, where the additional nitrogen atom is replaced by R ° and the heterocycle is optionally replaced by one or two R ⁴ ; R ³ is selected from hydrogen, Cs-ecicloalkyl, and Ci-salkyl, where Ci-salkyl is optionally substituted by -OH or -OCi-3alkyl; and R ⁴ is CI_ ₃ alkyl optionally substituted by -OH.
[0018] Specific R ³ values include, but are not limited to, hydrogen, cyclopropyl, cyclopentyl, cyclohexyl, methyl, ethyl, propyl, isopropyl, tert-butyl and hydroxyethyl.
[0019] Examples of R / ¹ values include, but are not limited to, methyl, ethyl and hydroxymethyl.
[0020] In another specific aspect, Rò is selected from hydrogen and Ci-aalkyl or R ¹ is C1-3alkyl and X is -C (O) R ² , where R ² is selected from
where R ³ is hydrogen or Ci-salkyl, where Ci-salkyl is optionally substituted by -OH; a is 0, 1, or 2; b is 1 or
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2; provided that when a is 0, R ^J is Cl - ₃ alkyl, Cl - ₃ alkyl which is optionally substituted by -OH; and R ' ¹ , when present [0021] Em. yet another aspect, R ¹ is selected a.
from hydrogen and Ci-alkyl or R '^; is i-3alkyl and X is - ~ C (O) R ² where R ^z is selected from

Where
R ^J is C1-3 alkyl or - (CH2) 2OH; R ^{J £} is C1-3 alkyl; a is 0 or and R ^4, when present, is Cl - ₃ alkyl.
[0022]
In a specific aspect,
R ¹ is selected from hydrogen and C _1-3 alkyl and X is -C (O) R ^Z , where R ² is NR ^lo R ~ ⁴ , where R ~ ³ and R ⁱ⁴ together with the nitrogen atom to which they are linked to form a 5- to 6-membered heterocycle, where the heterocycle is optionally substituted by -NR ° R ° and R ⁷ ; R ' ^J and R ⁶ are C _1-3 alkyl independently or and R ⁶ together with the nitrogen atom to which they are attached form a 5- or 6-membered heterocycle optionally including one. oxygen atom; and R ^z is i- ₃ alkyl, optionally substituted by a 5- or 6-membered heterocycle containing a nitrogen atom. In a particular ASPEC, R 'is a Cl - ₃ alqu.il, optionally substituted pyrrolidinyl.
[0023] In a specific aspect, R ¹ is selected from hydrogen and Ci-alkyl and X is -C (O) R ^Z where R ² is
wherein R 'and R ° are CI_ ₃ alkyl or R ⁵
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[0024] In another specific aspect, R ¹ is selected from hydrogen and Ci_ ₃ alkyl and X is -C (O) R ^Z where R ² is a group selected from:
or C ₃ alkyl, CI - ₃ alkyl which is optionally substituted by
OH; ae 0, 1, or 2; be .1. or 2; n,, quancto present, and Οχ ₃ alkyl; provided that when a is 0, R³ is CI_ ₃ alkyl, CI .. ₃ where alkyl is optionally substituted by -OH; R ³ and R ° are C ₃ alkyl independently or R ° and R ° together form - (0¾¾ ..
5-; and R 'is hydrogen or C ₃ alkyl.
[0025] In a specific aspect, R ¹ is selected from hydrogen and Ci_ ₃ alkyl and X is ~ C (O) R where R ² is - NR ¹³ R ¹⁴ , where R ¹³ and R ¹⁴ together with the atom of nitrogen to which they are attached form morpholinyl.
[0026] On. a specific aspect, R ¹ is selected from hydrogen and Ci_3alkyl and X is -C (O) R ^Z where R ² is NR ¹³ Rf ⁴ , where R ^{; o} is R ⁸ and R ¹⁴ is R ⁹ ; R ⁸ is hydrogen or Cq. 3 alkyl; R ⁹ e - (CiqqNR ^ R ¹¹ or a 4- to 6-membered heterocycle containing a nitrogen atom, where the nitrogen atom is replaced by R ¹² ; R ^1U and R ¹ * are Ci_3alkyl independently or R ¹⁰ and R ¹¹ together with c · nitrogen atom to which they are attached form a 5- or 6-membered heterocycle and R ¹² is Ci-3alkyl or C ₃ -6Ccycloalkyl, where Ci ₃ alkyl is optionally substituted by —OH.
[0027] On. another specific aspect, R ¹ is selected from hydrogen and Cq .. ₃ alkyl and X is -C (0) R ² where R ² is Lawyer´s Tariff No .: P-338-PCT
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NR ¹³ R ' ^l4 , where Rd' ³ is R ⁸ and R ⁱ⁴ is R ⁹ ; R ⁸ is Ci-alkyl; R ^y is (CH2) 2NR ^1u R · ¹ or piperidinyl, where piperidinyl is replaced on the nitrogen atom by R ¹ ; R ¹⁰ and R ^x ~ are C 1 -alkyl independently; and R ¹² is C ₃ or C aalquil _6cicloalquil where -C salquil is optionally substituted by -OH.
[0028] In another specific aspect, R ^± is selected from hydrogen and Ci-3alkyl and X is -C (O) R ² where R ² is NR ¹³ R ¹⁴ , where R ¹³ is R ⁸ and R ¹⁴ is R ⁹ ; R ⁸ is -CH3; R ⁹ is ~ (CH ₂ ) ₂ NR ⁱ⁰ R ¹¹
NR ¹² alkyl independently and R ⁱ² alkyl.
[0029] Where R iquil members and read an oxygen Cl - ₃ alkyl which is optionally substituted by ICI or --N-salquil) 2 / and a 5- or 6 - membered is optionally substituted by Cl - ₃ alkyl.
0030] In another specific aspect, R ¹ is selected from hydrogen and C _1-3 alkyl, and X is -C (O) R ² where R ^z is OR ¹³ , where R ¹³ is selected from C _{3- 3} alkyl , C3scicloalquil and a 5- or 6 members, including one heteroatom selected from nitrogen and oxygen, which is CI_ ₃ alkyl substituted with -OH and optionally one. heterocycle and 5 or 6 members is optionally substituted by C _1-3 alkyl.
[0031] In a specific aspect, R ¹ is selected from hydrogen and C _1-3 alkyl, and X is -CH ₂ R ¹⁶ , where R ^lc is NR ^x7 R ¹⁰ , where R ¹ and RC ⁸ are C1-4alkyl or C3-5c1cloalkyl.il
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14/136 independently or R ¹ 'and R ¹⁸ together with the nitrogen atom to which they are attached form a 5- or 6-membered heterocycle, optionally including an oxygen atom, where the heterocycle is optionally substituted by C1-3alkyl.
[0032] In outrt □ aspect hey spec Rf is selected a from hi hydrogen and Ci-akyl , θ X is -CH ₂ R ¹⁶ , where R ^1q is - NR ^lz R ⁱ⁸ , where R ¹⁷ and R ¹⁸ s a o C i - 4 to 1 q i lil or C.3-.5 cycle lqui.1
independently or R ^{1 /} and R ¹⁸ together with the nitrogen atom to which they are attached form a 5- or 6-membered heterocycle, where the heterocycle is optionally substituted by Ci-salquil.
[0033] In. one. specific aspect, R ¹ is selected from hydrogen and Ci-aalkyl, and X is -CH ₂ R ^1S , where R ¹⁶ is - OR, where R is hydrogen or Ci-aaiqun.
[0034] In a specific aspect, R ¹ is selected from - (CH ₂ ) 2NR ^z0 R ^zl and a 4- to 6-membered heterocycle containing a nitrogen atom, where one. nitrogen atom is optionally substituted by R ^z and X is -CH ₂ OR ^z8 , where R ^zU , R ^zz , R ²² and R ²³ are independently selected from hydrogen and Ci-alkyl.
[00.35] In a specific aspect, R ¹ is selected from - (CH ₂ ) ₂ NR ^zU R ²¹ and a 4- to 6-membered heterocycle containing a nitrogen atom, where a nitrogen atom is optionally substituted by R ^z2 and X is -C (O) OR ²⁴ , where R ²⁰ ,
R ~, R ²² and R ²⁴ are independently selected from hydrogen and Ci-3 alkyl.
[0036] In a specific aspect, R ¹ is selected from ”(CH ₂ ) ₂ NR ²⁰ R ²¹ ,
NR ²²
selected from -CH ₂ OR ²³ and -C (O) OR ²⁴ , where R ²⁰ , R ²¹ ,
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R ^z2, R ^28, and R ^z4 are independently selected hydrogen and left CI ₃ alkyl.
[0037] In another aspect, the invention provides a compound of formula (II):
Where:
R ¹ is Ci-alkyl;
R ² is a group selected from:
and - NR ⁸ R ^S , where
R '· is hydrogen or Ci_. ₃ alkyl, where Ci-aalkyl is optionally substituted by —OH, ã is 0, 1, OR 2, b is 1 or 2,
R ⁴ when present, is Ci-alkyl, provided that when a is 0, R ^J is C1-3alkyl, where ₃ alkyl is optionally substituted by —OH,
R ° and R ⁶ are independently Ci-3alkyl or R ⁸ and together with - (CH2) 4..5-,
R 'is hydrogen or alkyl CI_ _3,
R ⁸ is- CH ₃ ,
R ^s is - (CH ₂ ) ₂ NR ¹⁰ R ^í1 or
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Rd θ ⁰ ~ Rn _are independent emente _the C1-3alqu.il and _f
R ^{1 z} is C i - 3 to 1 qui 1;
or a pharmaceutically acceptable salt.
[0038] In another aspect, the invention provides a compound selected from the following compounds:
((S) -2- (6- (2-ethyl-5-fluoro-4-hydroxyphenyl) -lu-indazole-
3-yl) -5-propyl-4,5,6,7-tetrahydro-3H-imidazo [4,5-c] pyridin-6 yl) ((IS, 4S) -5 - methyl-2,5- diazabicyclo- [2.2.1] heptan-2- i1) methanone, ((S) -3- (dimethylamino) pyrrolidin-l-yl) ((S) -5-ethyl-2- (6 (2-ethyl-5 -fluoro-4-hydroxyphenyl) -1H-indazol-3-yl) -4,5,6,7tetrahydro-'377-imidazo [4,5-c] pyridin-6-yl) methanone, (S) - (2 - (6- (2-ethyl-5-fluoro-4-hydroxyphenyl) -1H-indazol-3 yl) -5-isopropyl-4,5,6,7-tetrahydro-3H-imidazo [4,5-c] pyridine-
6-yl) (4-methyl-1,4-diazepan-1-yl) methanone, ((S) -2- (6- (2-ethyl-5-fluoro-4-hydroxyphenyl) -lFindazol-
3-yl) -5-methyl-4,5,6,7-tetrahydro-3H-imidazo [4,5-c] pyridin-6yl) ((R) -4- (2-hydroxyethyl) -2-methyl- piperazin-1-yl) methanone, (S) - (2- (6- (2-ethyl-5-fluoro-4-hydroxyphenyl) -lu-indazol3-yl) -5-propyl-4,5,6,7 -tetrahydro-3H-imidazo [4,5-c] pyridin-6 yl) (4- (2-hydroxyethyl) piperazin-1-yl) methanone, and their pharmaceutically acceptable salts.
[0039] In yet another aspect, the invention provides a compound where the compound is ((S) -2,4-dimethylpiperazin-lil) ((S) -2- (6- (2-ethyl-5-fluoro-4 -hydroxyphenyl) -1H-indazol-3yl) -5-methyl1-4,5,6,7-tetrahydro-3H-imidazo [5,4-c] pyridin-6yl) methanone or a pharmaceutically acceptable salt c is useful.
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17/136 [0040] In yet another aspect, the invention provides a compound where the compound is (A) -N- (2- (diethylamino) ethyl) -5ethyl-2- (6- (2 ~ ethyl ~ 5-fluoro ~ 4 - hydroxyphenyl) "1H-indazol-3-yl) N-methyl-4,5,6,7-tetrahydro ~ 3H ~ imidazo [5,4-c] pyridine-6 ~ carboxamide or an acceptable salt from the view point of view.
[0041] On. yet another aspect, the invention provides one. compound where the compound is ((S) -2- (6- (2 ~ ethyl ~ 5 ~ fluoro-4 ~ hydroxyphenyl-1H-indazol-3-yl) -5-propyl-4,5,6,7-tetrahydro3H -imidazo [4,5-c] pyridin-6-yl) ((IS, 4S) -5-methyl1-2,5 diazabicyclo- [2.2.1] heptan-2-yl) methanone of the formula
report in accordance with 1UPAC conventions as
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18/136 implemented in the ChemDraw program (PerkinElmer, Inc.,
Cambridge, MA). For example, the compound of Example 1:
F
is designated as (((Sj-2- (6- (2-ethyl-5-fluoro “4-hydroxyphenyl) 1H-indazol-3-yl) -5-propyl-4,5,6,7-tetrahydro-3H- imidazo [4,5-
c] pyridin-6-yl) ((IS, 4S) -5-methyl-2,5-diazabicyclo- [2.2.1] heptan-2-yl) methanone.
[0045] Furthermore, the imidazo portion of the tetrahydroimidazopyridine group in the structure of formula (I) exists in the tautomeric forms, illustrated below for a fragment of the compound of Example 1
THE
B
According to the IUPAC convention, origins of different numbers of these representations give atoms of the imidazo1: 2 - (lA-indazol-3-yl) -4, b, 6,7-tetrahydro-imimazo [4, bc] pyridine (structure A) versus 2
1H — 1nda zo1 - 3 - yl) -4,5,6,7-tetrahydro-3H- imidazo [4,5-c] pyridine (structure
B). It should be understood that, although the structures are presented or named, in a particular form, the invention also includes its tautomer.
[0046] The compounds of the invention may contain one or more chiral centers and, therefore, such compounds (and their intermediates) may exist as racemic mixtures,
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Petition 870190088769, of 09/09/2019, p. 33/165 pure stereoisomers (eg enantiomers or diastereomers) mixtures enriched with stereoisomers and the like. Chiral compounds shown or named here without defined stereochemistry at a chiral center are intended for: any or all possible variations of stereoisomers in the undefined stereocenter, unless otherwise stated. The representation or appointment of one. certain stereoisomer means that the indicated stereocenter has the indicated stereochemistry with the understanding that small amounts of other stereoisomers may also be present, unless otherwise indicated, provided that the utility of the compound described or called is not eliminated by the presence of another stereoisomer.
[0047] The compounds of the formula (I) also have several basic groups (for example, amino groups) and, therefore, such compounds can exist as the free base or various forms of salt, as a monoprotonated, diprotonated, triprotonated salt form , or their mixtures. All of these forms are included in the scope of application of the present invention, unless otherwise stated.
[0048] The present invention includes there tan were composed of formula (I) labeled with isotopes, or be. compounds from formula (I) where one or more atoms was . subs replaced or enriched with an atom with the mesi ro r i humerus atomic but color
atomic mass different from that which predominates in nature.
Examples of isotopes that can be incorporated into a compound of formula (I) include, but are not limited to, ² H, ³ H, ⁿ C, ¹³ C, ¹⁴ C, ¹³ N, ¹⁵ N, ¹⁵ O, ¹⁷ O, ¹⁸ O. Of particular interest are the compounds of the formula (I) enriched with. tritium, or carbon-14, which can be used, for example, in.
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20/136 studies of tissue distribution. Of particular interest are also compounds of formula (I) enriched with. deuterium, especially in. a place of metabolism, whose compounds have greater metabolic stability. In addition, of particular interest are compounds of formula (I) enriched with a positron-emitting isotope, such as, for example, “C, ^lo 0 and ¹³ N, whose compounds can be used, for example, in tomography studies by Positron Emission (PET).
Definitions [0049] When describing a. present invention, including its various aspects and modalities, the following terms have the following meaning, unless otherwise indicated.
[0050] The term alkyl means a group of saturated monovalent hydrocarbons that can be linear or branched or combinations thereof. Unless otherwise defined, such alkyl groups typically contain 1 to 10 carbon atoms. Representative alkyl groups include, for example, methyl (Me), ethyl (Et), n-propyl (n-Pr) or (nPr), isopropyl (i-Pr) or (iPr), n-butyl (n-Bu) ) or (nBu), sec-butyl, isobutyl, tert-butyl (t-Bu) or (tBu), npentyl, n-hexyl, 2,2-dimethylpropyl, 2-methylbutyl, 3-methylbutyl, 2-ethylbutyl, 2 , 2-dimethylpentyl, 2-propylpentyl and the like.
[0051] When a given number of carbon atoms is assigned to a given name, the number of carbon atoms is shown before the name. For example, the term C1-3 alkyl means one. alkyl group with 1 to 3 carbon atoms, where the carbon atoms are in
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21/136 any chemically acceptable configuration, including linear or branched configurations.
[0052] The term cycloalkyl means one. saturated monovalent carbocyclic group that can be monocyclic or multicyclic. Unless otherwise defined, such cycloalkyl groups typically have 3 to 10 carbon atoms. Representative groups of cycloalkyl include, for example, cyclopropyl (cPr), cyclobutyl (cBu), cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, adamantyl and the like.
[0053] The term cpropyl means cyclopropyl.
[0054] The term heterocyclyl, heterocycle, heterocyclic or heterocyclic ring means one. saturated or partially unsaturated monovalent cyclic group with 3 to 10 total ring atoms, where the ring contains 2 to 9 carbon atoms and 1 to 4 hetero atoms selected from nitrogen, oxygen and sulfur. The groups can be heterocyclic monocyclic or.
multicyclic (ie joined or bridged). Representative groups of heterocyclics include, for example, pyrrolidinyl, piperidinyl, piperazinyl, imidazolidinyl, morpholinyl, thiomorphoryl, indolin-3-yl,
2-imidazolinyl, tetrahydropyranyl, 1,2,3,4tetrahydroisoquinolin-2-yl, quinuclidinyl, 7-azanorbornanil, nortropan.il, and the like, where the attachment point is on any carbon or nitrogen atom available in the ring. When the context makes the point of attachment of the heterocyclic group evident, such groups may alternatively be referred to as non-valiant species, i.e., pyrrolidine, piperidine, piperazine, imidazole, tetrahydropyran, etc.
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22/136 [0055] The term halo means fluoro, chloro, bromo [0056] The term therapeutically effective amount means an amount sufficient to treat when administered to a patient in need of treatment.
[0057] the term to treat or treat means to alleviate or suppress the medical illness or disorder to be treated (eg, a respiratory illness) a patient (particularly a human); or relieve symptoms of the medical condition, illness or medical disorder.
[0058] The term pharmaceutically acceptable salt means a salt that is acceptable for administration to a patient or mammal, such as a human (e.g., salts with acceptable safety in mammals for a given dosage schedule). Pharmaceutically acceptable salts include acetic, ascorbic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, edisyl, fumaric, gentisic, glyconic, glycuronic, glutamic, nipuric, hydrobromic, hydrochloric, isatoic, lactic, lactic, lactic, lactic, lactic, salts maleic, malic, mandelic, methanesulfonic, muscic, naphthalenesulfonic, naphthalene-1,5-disulfonic, naphthalene2,6-disulfonic, nicotinic, nitric, orotic, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric, and xenophenic.
[0059] The term its salt means a compound formed when the hydrogen of an acid is replaced by an cation, such as a metal cation or an organic cation and the like. For example, the cation can be a protonated form of a compound of formula (1), that is, a form
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23/136 where one or more amino groups were protonated by an acid. Normally, salt is a pharmaceutically acceptable salt, although this is not necessary for salts of intermediate compounds that are not suitable for administration to a patient.
[0060] The term amino protection group means an adequate protection group to prevent undesirable reactions in one. amino nitrogen. Representative groups of amino protection include, but are not limited to, formyl; acyl groups, for example, alkanoyl groups, such as acetyl and tri-fluoroacetyl groups; alkoxycarbonyl group, such as tert-butoxycarbonyl (Boc); arylmethoxycarbonyl groups, such as benzyloxycarbonyl (Cbz) and
9-fluorenylmethoxycarbonyl (Fmoc); arylmethyl groups, such as benzyl (Bn), trityl (Tr), and 1,1-di- <4 '~ methoxyphenyl) methyl; silyl groups, such as trimethylsilyl (TMS), tert -but ildimethylsil.il (TBDMS), [2 (trimethylsilyl) -ethoxy] methyl (SEM); and the like.
[0061] The term hydroxy protection group means an adequate protection group to prevent undesirable reactions in one. hydroxy group. Representative hydroxy protecting groups include, but are not limited to, alkyl groups, such as methyl, ethyl and tert-butyl; acyl groups, for example, alkanoyl groups, such as acetyl; arylmethyl groups, such as benzyl (Bn), p-methoxybenzyl (PMB), 9-fluorenylmethyl (Fm) and diphenylmethyl (benzhydryl, DPM); silyl groups, such as trimethylsilyl (TMS) and tert-butyldimethylsilyl (TBS); and the like.
[0062] Numerous protecting groups and their introduction to removal are described in. T. W. Greene and P.G.M. Wuts,
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Protecting Groups in Organic Synthesis, third edition, Wiley, New York
General synthetic procedures [0063] The compounds of this invention, and their intermediates, can be prepared according to the following general methods and procedures using commercially available or routinely prepared starting materials and reagents. Substituents and variables (for example, R ¹ , R ^z , R ³ , R ⁴ , etc.) used in the following schemes have the same meaning as those defined in this document, unless otherwise indicated. In addition, compounds with an acid or basic atom or functional group can be used or can be produced as a salt, unless otherwise indicated (in some cases, the use of a salt in a particular reaction will require conversion of the salt for the form, do not salt, for example, a free base, using routine procedures before performing the reaction).
[0064] Although a particular embodiment of the present invention can be shown or described in the following procedures, those skilled in the art will recognize that other embodiments or aspects of the present invention can also be prepared with such procedures, or using other methods, reagents and materials known to those skilled in the art. In particular, it will be appreciated that the compounds of the invention can be prepared by various process routes in which the reagents are combined in different orders to provide different intermediates in the route and to produce the final products.
[0065] A general method of preparing the final compounds of the invention in which variable X is defined as Lawyer's Tariff No .: P-338-PCT
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C (O) PÚ and R * is C1-3alkyl.U uses a key intermediate 1 and an amine of formula 2 as illustrated commonly in Scheme 1 and, in particular, for an example where R is defined as

2a to specifically exemplify a final amide product representing formula (II).
Layout 1
(II)
[0066] To prepare the amide compounds of formula (II), the carboxylic acid of Formula 1 was reacted with amine 2 according to. typical conditions of amide bond formation. Typically, carboxylic acid 1 comes in contact with about 1 to 4 equivalents of amide 2 in the presence of an excess of base. As shown in the examples below, the amide bond formation reaction can use coupling agents, such as hexafluorophosphate.
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6/1 ci 6
Ν, Ν, Ν ’, Ν'-tetramethyl-O- (7-azabenzotriazol-1-yl) uronium (HATU) or other acyclinate amide acyent.es known in the. technical. The reaction is carried out at room temperature between about 2 and 24 hours or. until the reaction is considerably completed.
[0067] Compounds in which variable X is defined as -C (O) OR ^lj can be prepared by an esterification reaction of the carboxylic acid of formula 1 with an alcohol of formula HO-R ¹⁵ where acid 1 enters. contact with a large excess of alcohol, for example, 25 alcohol equivalents, in the presence of a coupling reagent such as HATU and an excess of base. Where R ¹⁵ is defined as one. heterocycle substituted by Ci-aalkyl, an alcohol reagent without substitute · alkyl can be used in the esterification reaction and an alkyl substitute is added in a subsequent step.
[0068] Formula 1 carboxylic acid can be prepared as shown in Scheme 2
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Pg ' ¹ represent different amino protecting groups. As described in the examples below, a useful choice of protecting groups is benzyl or methyl like Pg ¹ , tetrahydropyranyl (THP) like Pg ¹ , tert-butoxycarbonyl ÍBoc) or benzyl like Pg ^3, and [2- (trimethylsilyl) ethoxy ] methyl (SEM) as Pg ⁴ . The first step in Scheme 2 is the coupling of catalyzed palladium
Stille of intermediate 3 with intermediate 4, where intermediate 3 phenyl-indazole has the trimethylstannyl portion and
The reaction partner 4 is the substituted iodine. The reaction is conducted at elevated temperature, for example, between 80 ° C and
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180 ° C for about 10 to 24 hours or until the reaction is considerably complete.
[0069] When benzyl is used as Pg ¹ , in the next step, the methyl ester of intermediate s 5 is converted to. one. benzyl ester in intermediate 6 by the reaction of 5 with. benzilic alcohol. Both benzyl protecting groups are conveniently removed by hydrogenation of the catalyzed palladium to provide the intermediate 7 which can be completely deprotected by reaction with acid, typically hydrochloric acid. In a final step, the substitute R ¹ is added by the reductive alkylation of intermediate 8 with a reagent R ^{± a} where R ^la is an aldehyde or ketone defined in such a way that with the reaction, Rt is produced For example, to add a substitute methyl R ¹ , formaldehyde is used as reagent R ^id , to add an isopropyl moiety as a substituent R ¹ , acetone is used as reagent R ^ld . A. The reaction is generally conducted in the presence of a reducing agent such as sodium cyanoborohydride or sodium triacetoxyborohydride or similar at room temperature for a period of about 10 to about 24 hours or until the reaction is substantially complete.
[007 0] Intermediates 3 and 4 can be prepared from commercial or easily prepared starting materials, as described in detail below. In particular, a process for preparing intermediate 3 in which Pg is benzyl and Pg ° is THP using the Suzuki-Miyaura coupling of compound 9 with compound 10 followed by conventional reactions to add the trimethylstannyl group.
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of compound 11, which is commercially available in racemic and stereospecific forms and can also be prepared from histidine.
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30/136 [0073] As shown in Scheme 3 _; substitute CH2NR ¹⁷ R ^1B is added to a protected intermediate '12 to form an intermediate 14, which is completely unprotected, for example, by reaction with boron tribromide, to form intermediate 15. In the final step, substitute R ¹ is added by reductive alkylation as described in Scheme 2.
[0074] The aldehyde intermediate 12 is also useful for the preparation of final compounds of the invention in which X is defined as -CH ₂ OH. Compound 12 can also be in contact with one. reducing agent such as sodium borohydride to provide an intermediate analogous to compound 14 having CH ₂ OH in place of -CH ₂ NR ¹⁷ R ^lb , which may be completely unprotected and then react with an R ^{± d} reagent as in Scheme 3 to provide the compounds in which X is -ChibOH · [0075] The aldehyde intermediate 12 can be prepared by using the Weinreb-Nahm reaction as shown in Scheme 4.
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31/136 [0076] The intermediate amide 17, which is prepared by reacting compound 16 with dimethylhydroxylamine, is selectively reduced to aldehyde 12 upon contact with lithium aluminum hydride. A. The reaction is conducted at a low temperature, for example, between -60 ° C and - 80 ° C for about 1 to 3 hours or until the reaction is considerably complete.
[0077] So in one. aspect of the method, the invention provides a process for preparing a compound of formula (II) or a pharmaceutically acceptable salt, the process comprising reacting a compound of formula 1 with a compound of formula 2, as illustrated in Scheme 1 for providing a compound of formula (II) or a pharmaceutically acceptable salt.
[0078] In yet another aspect of the method, the invention provides a process for preparing a compound of formula 1, the process comprising reacting a compound of formula 8 with R ^xa in the presence of a reducing agent, where R ^{;,: i} is an aldehyde or ketone so defined by the reducing alkylation of the substitute R ¹ where R ¹ is C1-3 alkyl, is attached to the compound of formula 8 to provide the compound of formula 1.
[0079] In a further aspect of the method, the invention provides a process for preparing a compound of formula 8, the process comprising deprotecting a compound of formula 7.
[0080] In yet another aspect, the invention provides a compound of formula 1 and compounds of formula 7 and 8 useful in preparing a compound of formula 1.
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Pharmaceutical Compositions [0081] The compounds of the invention and their pharmaceutically acceptable salts are normally used in the form of a pharmaceutical composition or formulation. Such pharmaceutical compositions can advantageously be administered to a patient by inhalation. In addition, the pharmaceutical compositions can be administered to one. patient by any means. acceptable administration, including, but not limited to, oral, rectal, nasal, topical (including transdermal), inhalation and types of parenteral administration.
[0082] Consequently, in one of its composition aspects, the invention is directed to a pharmaceutical composition comprising a pharmaceutically acceptable carrier or excipient and a compound of the formula (I), where, as defined above, compound of the formula (I), means a compound of. formula (I) or its pharmaceutically acceptable salt. Optionally, such pharmaceutical compositions can have other formulation agents and / or. therapeutic if desired. When we talk about compositions and their uses, the compound of the invention can also be referred to here as the active agent. As used herein, the term compound of the invention is intended to include all compounds covered by formula (I), as well as the species contained in formula (II) and their pharmaceutically acceptable salts.
[0083] The pharmaceutical compositions of the invention have one. therapeutically effective amount of one. compound of the present invention. Those skilled in the art will recognize, however, that a pharmaceutical composition may have
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 47/165 ό 3 1 ò more than one effective therapeutic amount, that is, bulk compositions or less than one effective therapeutic amount, that is, individual unit doses designed for the administration of multiple doses to achieve a therapeutic amount, satisfies.
[0084] Typically, such pharmaceutical compositions will contain about 0.01 to 95%, by weight, of the active agent, including, for example, from about 0.05 to 30%, by weight, of the active agent and from 0.1% to about 10% by weight of the active agent.
[0085] Any conventional carrier or excipient can be used in the pharmaceutical compositions of the invention.
The choice of a particular carrier or excipient, or combinations of dealers or excipients, will depend on the mode of administration to be used to treat a particular patient or type of illness or medical condition. In this context, the preparation of a pharmaceutical composition suitable for a particular mode of administration is well within the scope of those skilled in the pharmaceutical art. In addition, the dealers or excipients used in the pharmaceutical compositions of this invention are commercially available. By way of another illustration, conventional formulation techniques are described in
Remington: The Science and Practice of Pharmacy, 20th
Edition, Lippincott Williams & White, Baltimore, Mariland (2000); and HC Ansel et al., Pharmaceutical Dosage Forms and Drug Delivery Systems, 7 ^th Edition, Lippincott Williams & White, Baltimore, Maryland (1999).
[0086] Representative examples of materials that can serve as acceptable carriers from the point of view
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Pharmaceutical 34/136 include, but are not limited to, the following: sugars, such as lactose, glucose and sucrose; starches, such as corn and starch; cellulose, such as microcrystalline cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatine; baby powder; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cotton oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar; buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline solution; Ringer's solution; ethyl alcohol; phosphate buffer solutions; and other compatible non-toxic substances used in pharmaceutical compositions.
[0087] Pharmaceutical compositions are generally prepared by stirring or mixing the active agent thoroughly and efficiently with. a carrier and one or more pharmaceutically accepted optional ingredients. The resulting uniformly stirred mixture can then be molded or loaded into tablets, capsules, tablets and using conventional procedures and equipment.
[0088] In one aspect, the pharmaceutical composition of the invention is suitable for inhalation administration. Pharmaceutical compositions suitable for administration by inhalation are typically in the form of an aerosol or powder. Such compositions are generally administered using devices
Lawyer No .: P-338-PCT
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[0089] In. in a particular embodiment, the pharmaceutical composition is administered by inhalation through a dry powder inhaler. Such dry powder inhalers normally deliver the pharmaceutical composition as a free flowing powder that is dispersed in the patient's air flow during inspiration. To obtain a free flowing powder composition, the therapeutic agent is usually formulated with a suitable excipient such as lactose, starch, mannitol, dextrose, polylactic acid (PLA), poly (lactic acid ~ glycolic acid) (PLGA) or their compounds. combinations. Typically, the therapeutic agent is micronized and combined with a suitable carrier to form a composition suitable for inhalation.
[0090] A representative pharmaceutical composition for use in a dry powder inhaler comprises lactose and a compound of the invention in micronized form. Such a dry powder composition can be prepared, for example, by combining dry and crushed lactose with. the therapeutic agent and then mix the compounds dry. The composition is usually loaded in a dry powder dispenser, in inhalation cartridges or capsules for use in a dry powder release device.
[0091] Dry powder inhaler devices suitable for delivering therapeutic agents are described in the art and examples of such devices are commercially available. For example, dry powder inhaler delivery devices or products include Aeolizer (Novartis); Airmax (IVAX); ClickHaler (Innovata Biomed); Diskhaler (GlaxoSmithKline); Diskus / Accuhaler
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 50/165 (GlaxoSmithKline); Ellipta (GlaxoSmithKline); Easyhaler (Orion Pharma); Eclipse (Aventis); FlowCaps (Hovione); Handihaler (Boehringer Ingelheim); Pulvinal (Chiesi); Rotahaler (GlaxoSmithKline); SkyeHaler / Certihaler (SkyePharma); Twisthaler (Schering-Plow); Turbuhaler (AstraZeneca); Ultrahaler (Aventis) and the like.
[0092] In another particular embodiment, the pharmaceutical composition is administered by inhalation through a metered-dose inhaler. Such metered-dose inhalers discharge a measured amount of a therapeutic agent using one. compressed propellant gas. Thus, pharmaceutical compositions administered using a metered-dose inhaler typically comprise a solution or suspension of the therapeutic agent in a liquefied propellant. Any suitable liquefied propellant can be used including hydrofluoralkanes (HFAs), such as 1,1,1,2-tetrafluoroethane (HFA
134a) and 1,1,1,2,3,3,3-heptafluoro ~ n-propane, (HFA 227); and chlorofluorocarbons, such as CCI3F. In a particular embodiment, the propellants are hydrofluoralkanes. In some embodiments, the hydrofluoralkane formulation contains a cosolvent, such as ethanol or pentane and / or a surfactant, such as sorbinate trioleate, oleic acid, lecithin and glycerin.
[0093] A representative pharmaceutical composition for use in one. metered-dose inhaler has about 0.01% to about 5% in. weight of a compound of the invention; from about 0% to about 20% by weight of ethanol and from about 0% to about 5% by weight of surfactant with the remainder being an HFA propellant. Such compositions are usually prepared by adding refrigerated or pressurized hydrofluoralkane to one. appropriate container containing the agent
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37/136 therapeutic, ethanol (if present) and surfactant (if present). To prepare the suspension, the therapeutic agent is micronized and then combined with the propellant. The composition is then loaded into. an aerosol container, which normally forms part of a metered-dose inhaler device.
[0094] Metered inhaler devices suitable for delivering therapeutic agents are described in the art and examples of such devices are commercially available. For example, metered-dose inhaler devices or representative products include AeroBid Inhaler System. (Forest Pharmaceuticals); Atrovent Inhalation Aerosol (Boehringer Ingelheim.); Flovent (GlaxoSmithKline); Maxair Inhaler (3M); Proventil Inhaler (Schering); Serevent Inhalation Aerosol (GlaxoSmithKline) and the like.
[0095] In another particular aspect, the pharmaceutical composition is administered by inhalation through one. nebulizer inhaler. Such nebulizer devices generally produce a high-speed vapor that causes air. the pharmaceutical composition to spray like a mist that is carried to the patient's respiratory tract. Thus, when formulated for use in an inhaler nebulizer, the therapeutic agent can be dissolved in a suitable dealer to form a solution. On the other hand, the therapeutic agent can be micronized or nanotriturated and combined with a suitable carrier to form a suspension.
[0096] A representative pharmaceutical composition for use in a nebulizer inhaler comprises a solution or suspension comprising about 0.05 µg / ml to about 20 mg / ml of a compound of the invention and excipients
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38/136 compatible with nebulized formulations. In one embodiment, the solution has a pH of about 3 to about 8.
[0097] Nebulizer inhaler devices suitable for delivering therapeutic agents are described in the art and examples of such devices are commercially available. For example, nebulizer devices or representative products include. Respimat Softmist Inhalaler (Boehringer Ingelheim); AERx Pulmonary Delivery System (Aradigm Corp.); PARI LC Plus Reusable Nebulizer (Pari GmbH) and the like.
[0098] In yet another aspect, the pharmaceutical compositions of the invention may also be prepared in a pharmaceutical form intended for oral administration. Pharmaceutical compositions suitable for oral administration may be in the form of capsules, tablets, lozenges, pills, pills, powders, granules, or as a solution or suspension in one. aqueous or non-aqueous liquid; or as a liquid oil-in-water emulsion or water-in-oil emulsion; or as an elixir or syrup and the like; cad; one containing a predetermined amount of a compound of the present invention as an active ingredient.
[0099] When intended for oral administration in a solid pharmaceutical form, the pharmaceutical compositions of the invention will generally include the active agent and one or more pharmaceutically acceptable carriers, such as sodium citrate or dicalcium phosphate.
Optionally or alternatively, such solid dosage forms may also comprise: fillers or extenders, binders, humectants, solution retarding agents, absorption accelerators, wetting agents,
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39/136 absorbents, lubricants, coloring agents and buffering agents. Release agents, coatings, sweeteners, sweeteners and flavors, preservatives and antioxidants may also be present in the composition of pharmaceutical compositions of the invention.
[00100Ί Alternative formulations may also include controlled-release formulations, liquid dosage forms for oral administration, transdermal patches and parenteral formulations. Conventional excipients and methods of preparing such alternative formulations are described, for example, in the reference by Remington, cited above.
The following non-limiting examples illustrate pharmaceutical compositions representative of the present invention.
Dry powder composition [00102] A micronized compound of formula (I) (1 g) is mixed with crushed lactose (25 g). This combined mixture is then loaded into individual blisters from an easily opened blister pack in an amount sufficient to provide between about 0.1 mg to 4 mg of the compound of formula I per dose. The contents of the blisters are administered using a dry powder inhaler.
Dry powder composition [00103] A micronized compound of formula (I) (1 g) is mixed with crushed lactose (20 g) to form a bulk composition having a 1:20 weight ratio of the compound to the crushed lactose. The combined composition is packaged in. a dry powder inhalation device capable of delivering between about 0.1 mg to about 4 mg of the compound of formula I per dose.
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Composition of the metered dose inhaler [00104] A micronized compound of formula (I) (10 g) is dispersed in a solution prepared by dissolving lecithin (0.2 g) in demineralized water. (200 ml). The resulting suspension is spray dried and micronized to form a micronized composition comprising particles with an average diameter of less than 1.5 µm. The micronized composition is then loaded into metered-dose inhaler cartridges containing 1,1,1,2-tetrafluoroethane pressurized in an amount sufficient to provide about 0.1 to about 4 mg of the compound of formula I per dose when administered by a metered-dose inhaler. .
Nebulizer composition [00105] A compound of formula (I) (25 mg) is dissolved in a solution containing 1.5-2.5 equivalents of hydrochloric acid, followed by the addition of sodium hydroxide to adjust the pH to 3.5 to 5.5 and 3% in. glycerol weight. A. The solution is stirred well until all components are dissolved. The solution is administered with a nebulizer device that provides about 0.1 mg to about 4 mg of the compound of formula I per dose.
Utility [00106] The inhibitor JAK inhibitors of the invention were designed for the treatment of fibrotic and inflammatory disease of the respiratory tract. In particular, the compounds were designed to allow delivery of a potent anti-cytokine agent directly to the site of action of respiratory disease in the lung while limiting systemic exposure.
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41/136 [00107] The compounds of the invention have been shown to be potent inhibitors of the JAK enzyme family: JAK1, JAK2, JAK3 and TYK2. In addition, the compounds demonstrated potent inhibition of pro-inflammatory and pro-fibrotic cytokines without exhibiting cytotoxicity in cell assays. It has been recognized that the broad anti-inflammatory effect of JAK inhibitors can suppress the function of normal immune cells, potentially leading to an increased risk of infection. The present compounds have been optimized to limit absorption from the lung into the plasma, thereby minimizing the risk of immunosuppression.
[001081 As described in the experimental section below, the absorption and distribution profiles of typical compounds in pre-clinical trials were made. Selected compounds tested in mice showed, at the same time, high concentration in lung tissue and low absorption in plasma. The compounds tested in mice exhibited exposure in the lung of one to two orders of magnitude greater than exposure in plasma. The compounds also showed retention in the mouse lung as evidenced by a longer lung half-life of about 5 hours. Importantly, it has been shown that the concentration of the substance in the mouse lung has a correlation with the predicted pharmacodynamic effect of inhibition of the enzyme JAK. The compounds of the invention have been shown to inhibit an effect of the proinflammatory cytokine IL-13 on mouse lung tissue. Specifically, the compounds demonstrated dose-dependent inhibition and concentration of IL-13-induced STAT6 phosphorylation in lung tissue that provides evidence of local involvement of the lung JAK target in
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[00109] The compounds have been shown to exhibit potent inhibitory activity at the cellular level and significant retention in lung tissue. Extensive research by the present inventors has determined that although it is possible to identify compounds that are potent at the cellular level or compounds that have significant retention in the lung, it is much more difficult to discover compounds that exhibit both desirable characteristics at the same time.
[00110] The anti-inflammatory activity of JAK inhibitors has been demonstrated in preclinical models of asthma (Malaviya et al., Int Immunopharmacol, 2010, 10, 829, -836;
Matsunaga et al., Biochem and Biophys Res Cominun, 2011, 404, 261-267; Kudlacz et al., Eur J Pharmacol, 2008, 582, 154161.) Thus, the compounds of the invention are expected to be useful for the treatment of inflammatory respiratory disorders, in particular asthma. Inflammation and fibrosis of the lung is characteristic of respiratory diseases other than asthma such as chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), pneumonitis, interstitial lung diseases (including idiopathic pulmonary fibrosis), acute lung injury, respiratory distress syndrome acute, bronchitis, emphysema, obliterating bronchiolitis and sarcoidosis. The present compounds are therefore expected to be useful for the treatment of chronic obstructive pulmonary disease, cystic fibrosis, pneumonitis, interstitial lung diseases (including idiopathic pulmonary fibrosis), injury
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 57/165 acute pulmonary, acute respiratory distress syndrome, bronchitis, emphysema, obliterating bronchiolitis and sarcoidosis.
[00111] The compounds of the disclosure demonstrated, inhibition of human T cell activation, inhibition of cytokines associated with inflammation and activity on human eosinophils and in models of pulmonary eosinophilia in mice. Therefore, the compounds of the disclosure are likely to be useful for the treatment of certain specific respiratory diseases.
[00112] Eosinophilic inflammation of the airways is a characteristic of diseases collectively called pulmonary diseases (Cottin et al., Clin. Chest. Med., 2016, 37 (3), 535-56). Eosinophilic diseases are associated with IL-4, IL-13 and IL-5 signaling. Eosinophilic lung diseases include infections (especially helminth infections), drug-induced pneumonitis (induced, for example, by therapeutic drugs such as antibiotics, phenytoin or 1-tryptophan), fungal-induced pneumonitis (eg, allergic bronchopulmonary aspergillosis), pneumonitis hypersensitivity and eosinophilic granulomatosis with polyangiitis (formerly known as ChurgStrauss syndrome). Eosinophilic pulmonary diseases of unknown etiology include acute idiopathic eosinophilic pneumonia, chronic idiopathic eosinophilic pneumonia, hypereosophilic syndrome and Löffler syndrome. The compounds of the disclosure have been shown to significantly reduce pulmonary eosinophilia in a rodent airway model and potentially inhibit IL-13, IL-4 and IL-2 signaling in. cellular assays. In addition, it has been shown that
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44/136 compound of example 1 potentially inhibited IL-5 mediated human eosinophilic survival.
[00113] A polymorphism in the IL-6 gene has. been associated with elevated levels of 1L-6 and an increased risk of developing pulmonary arterial hypertension (PAH) (Fang et al., J Am Soc Hypertens., 2017, 11 (3), 171-177). Corroborating with. the role of IL-6 in PAH, inhibition of the IL-6 receptor chain gpl30 improved the disease in a mouse model of PAH (Huang et al., Can J Cardiol., 2016, 32 (11), 1356. el-1356.elO). The compounds of examples 1 and 3 have been shown to inhibit IL-6 signaling.
[00114] Cytokines such as IFNy, IL-12 and IL-6 cytokines are involved in a variety of non-allergic lung diseases such as sarcoidosis and lymphangioleiomyomatosis (ElHashemite et al .. Am. J. Respir. Cell Mol. Biol., 2005, 33 , 227-230, and El-Hashemite et al., Cancer Res., 2004, 64, 34363443). The compounds of examples 1 and 3 have been shown to inhibit IL-6 signaling.
[00115] Bronchiectasis and infiltrative lung diseases are chronic diseases associated with chronic neutrophilic inflammation. The compounds of examples 1 and 3 have been shown to inhibit cytokines that are associated with neutrophilic inflammation (e.g., IL-6).
[00116] The pathological activation of T cells is fundamental in the etiology of several respiratory diseases. Autoreactive T cells play a role in bronchiolitis obliterans with organizing pneumonia (also called BOOP). Similar to BOOP, the etiology of lung transplant rejections is associated with an aberrant activation of receptor T cell activation by the lung
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 59/165 transplanted from the donor. Lung transplant rejections can occur early as primary graft dysfunction (PGD), organizing pneumonia (PO), acute rejection (RA) or lymphocytic bronchiolitis (BL) or they can occur years after lung transplantation as chronic lung graft dysfunction (CLAD). CLAD was previously known as bronchiolitis obliterans (BO), but is now considered a syndrome that can have different pathological manifestations including BO, restrictive CLAD (rCLAD or RAS) and graft neutrophilic dysfunction. Chronic pulmonary graft dysfunction (CLAD) is a major challenge for the long-term management of lung transplant recipients since it causes the transplanted lung to gradually lose its functionality (Gauthier et al., Curr Transplant Rep., 2016, 3 (3), 185-191). CLAD is not very sensitive to treatment and, therefore, there remains a need for effective compounds capable of preventing or treating this condition. Several JAK-dependent cytokines, such as IFNy and IL-5, are overregulated in CLAD and in lung transplant rejection (Berastegui et al, Clin Transplant. 2017, 31, el2898). In addition, high levels of pulmonary CXCR3 chemokines such as CXCL9 and CXCL10 that are downstream of JAK-dependent IFN signaling are related to worse outcomes in patients undergoing lung transplantation (Shino et al, PLGS One, 2017, 12 (7) , e0180281). Systemic JAK inhibition has been shown to be effective in rejection of kidney transplantation (Vicenti et al., American Journal of Transplantation, 2012, 12, 2446-56). Therefore, JAK inhibitors have the potential to be effective in treating or preventing lung transplantation and CLAD.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 60/165 ό / 1 ό
Events similar to the activation of T cells as described as the basis for lung transplant rejection are also considered to be the main factor of graft versus lung host disease (GVHD) that can occur after hematopoietic stem cell transplantation. Similar to CLAD, pulmonary GVHD is a progressive condition with extremely poor outcomes and there is no currently approved treatment. A multicenter, retrospective research study of 95 patients with acute or chronic steroid refractory GVHD who received the systemic JAK inhibitor ruxolitinib as rescue therapy demonstrated a complete or partial response to ruxolitinib in most patients, including those with pulmonary GVHD (Zeiser et al, Leukemia, 2015, 29, 10, 2062-68). As systemic JAK inhibition is associated with serious adverse events and a small therapeutic index, there remains a need for a non-systemic JAK inhibitor, directed to the lung to prevent / or treat lung transplant rejection or lung GVHD. The disclosure compounds have the necessary characteristics to meet this need. More recently, the immune checkpoint inhibitor induced pneumonitis, another T cell-mediated lung disease has emerged with the increasing use of immune checkpoint inhibitors. Cancer patients treated with these T-cell stimulating agents can develop fatal pneumonitis. Certain compounds in the disclosure have been shown to inhibit anti-CD3 and IL-2-induced release of IFNy from activated T peripheral blood isolated T cells and thus have
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47/136 potential to present a new treatment for these sciences and notions. c o r i s s g r a v e s d e s a s s _l s t i d a s.
[00117] In one aspect, therefore, the invention provides a method for treating a respiratory disease in one. mammal (e.g., human), the method comprising administering to the mammal a compound of the invention or a pharmaceutical composition comprising one. pharmaceutically acceptable carrier and a compound of the invention.
[00118] In one aspect, respiratory disease is asthma, chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), pneumonitis, interstitial lung diseases (including idiopathic pulmonary fibrosis), acute lung injury, acute respiratory distress syndrome, bronchitis , emphysema, obliterating bronchiolitis and sarcoidosis. In another aspect, respiratory disease is asthma or chronic obstructive pulmonary disease.
[00119] On. one aspect, lung disease is a lung infection, an eosinophilic disease, a helminth infection, pulmonary arterial hypertension, sarcoidosis, lymphangioleiomyomatosis, bronchiectasis, an inf11tractive lung disease, drug-induced pneumonitis, fungal-induced pneumonitis, allergic bronchopulmonary bronchopulmonary pneumonia, allergic pneumonitis hypersensitivity, eosinophilic granulomatosis with polyangiitis, idiopathic acute eosinophilic pneumonia, idiopathic chronic eosinophilic pneumonia, hypereosinophilic syndrome, Löffler syndrome, bronchiolitis obliterans with organizing pneumonia, acute and chronic lung rejections (including PGD, OP, LB, AR and CLAD Restrictive CLAD and neutrophilic graft dysfunction), graft-versus-versus-host disease, bronchiolitis
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48/136 obliterating with organizing pneumonia, pulmonary artery hypertension, bronchiectasis, or pneumonitis induced by immunological checkpoint inhibitor.
[001201 The invention also provides a method for treating asthma in a mammal, the method comprising administering to the mammal a compound of the invention or a pharmaceutical composition comprising one. pharmaceutically acceptable carrier and a compound of the invention.
[001211 When used to treat asthma, the compounds of the invention will be administered orally in a single daily dose, or in multiple doses per day, although other forms of administration may be used. The amount of active agent administered per dose or the total amount administered per day will be determined by a doctor, considering the relevant circumstances, including the disease to be treated, the chosen route of administration, the compound in. question administered and its activity, age, weight, and the response of each patient, severity of the patient's symptoms and the like.
[001221 The invention further provides a method for treating a respiratory disease (including, but not limited to, the disease described herein) in a mammal, the method comprising administering to the mammal a therapeutically efficient amount of a compound of the invention or a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of the invention.
[001231 When used for the treatment of a respiratory disease (including, but not limited to, the disease described here), the compounds of the invention will be administered in. single daily dose or. in multiple doses per day, although they may
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49/136 other forms of administration may be used. The amount of active agent administered per dose or the total amount administered per day will be determined by a doctor, considering the relevant circumstances, including the disease to be treated, the chosen route of administration, the compound in question administered and its activity, the age, weight and a. response of each patient, the severity of the patient's symptoms and the like.
[00124] As JAK inhibitors, the disclosure compounds can also be useful for a variety of other diseases. The compounds of the disclosure may be useful for various gastrointestinal inflammatory indications that include, but are not limited to, inflammatory bowel disease, ulcerative colitis (proctosigmoiditis, pancolitis, ulcerative proctitis and left colitis), Cronn's disease, collagenous colitis, colitis lymphocytic, Behcet's disease, celiac disease, colitis induced by pont inhibitor. of immunological verification, ileitis, eosinophilic esophagitis, colitis related to graft versus host disease and infectious colitis. Ulcerative colitis (Reimund et al., J Clin Immunology, 1996, 16, 144-150), Cronn's disease (Woywodt et al., Eur J Gastroenterology Hepatology, 1999, 11, 267-276), collagenous colitis (Kumawat et al ., Mol Immunology, 2013, 55, 355-364), lymphocytic colitis (Kumawat et al., 2013), eosinophilic esophagitis (Weinbrand-Goichberg et al., Immunol Res, 2013, 56, 249-260), colitis related to graft versus host disease (Coghill et. al., Blood, 2001, 117, 32683276), infectious colitis (Stallmach et al., Int J Colorectal Dls, 2004, 19, 308-315), Behcet disease (Zhou et al. , Autolirmun Rev, 2012, 11, 699-704), celiac disease (by Nitto
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50/136 et al., World J Gastroenterol, 2009, 15, 4609-4614), inhibitor-induced colitis (checkpoint) immunological checkpoint (eg, CTLA-4 inhibitor-induced colitis; (Yano et al ., J Translation Med, 2014, 12, 191), or PD-1- or PD-L1 inhibitor-induced colitis), and ileitis (Yamamoto et a ±., Dig niver uís, 2008, 40, zo3 — z59) are characterized by elevated levels of some pro-inflammatory cytokines. As many pro-inflammatory cytokines signal via JAK activation, the compounds described in this application may be able to relieve inflammation and provide relief for your symptoms. In particular, the compounds of the disclosure may be useful for inducing and maintaining remission of nonspecific ulcerative colitis, and for the treatment of Crohn's disease, immunological checkpoint inhibitor-induced colitis and the gastrointestinal adverse effects of graft versus host disease. In one aspect, therefore, the invention provides a method for treating an inflammatory gastrointestinal disease in a mammal (e.g., human), the method comprising administering to the mammal a compound of the invention or a pharmaceutically acceptable salt and a . a compound or a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of the disclosure or a pharmaceutically acceptable salt.
[00125] Atopic dermatitis and other inflammatory skin diseases are associated with elevated pro-inflammatory cytokines that depend on the JAK-STAT pathway. Therefore, the compounds of the disclosure or a pharmaceutically acceptable salt can be beneficial in various dermal inflammatory or itchy conditions, which
Lawyer No .: P-338-PCT
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51/136 include, but are not limited to, atopic dermatitis, alopecia areata, vitiligo, psoriasis, dermatomyositis, cutaneous T-cell lymphoma (Netchiporouk et al., Cell Cycle. 2014; 13, 3331-3335) and subtypes ( Sezary, fungoid mycosis, pagetoid reticulosis, loose granulomatous skin, lymphomatous papulosis, chronic lichenoid pityriasis, lichenoid and varioliformis acuta, cutaneous CD30 + T-cell lymphoma, secondary CD30 + cutaneous lymphoma, non-mycotic large cell lymphoma, cutaneous lymphoma of large cells CD30-, pleomorphic T-cell lymphoma, Lennert's lymphoma, subcutaneous T-cell lymphoma, angiococyte lymphoma, blast NK cell lymphoma), prurigo nodularis, lichen planus, primary localized cutaneous amyloidosis, bullous pemphigus, cutaneous manifestations of graft versus disease host, pemphigoid, discoid lupus, granuloma annulare, chronic lichen simplex, vulvar / scrotal / perianal pruritus, lichen sclerosus o, post-herpetic neuralgia itching, lichen planus pillar and decalvating folliculitis. In particular, atopic dermatitis (Bao et al., Jak-STAT, 2013, 2, N24137), alopecia areada (Xing et al., Nat Med. 2014, 20, 1043-1049), vitiligo (Craiglow et al, J .Wi Dermatol. 2015, 151, 1110-1112), nodular prurigo (Sonkoly et al., J Allergy Clin Immunol. 2006, 117, 411-417), lichen planus (Welz-Kubiak et al., J Imraunol R.es , 2015, ID: 854747), localized primary cutaneous amyloidosis (Tanaka et al., Br J Dermatol. 2009, 161, 12171224), bullous pemphigoid (Feliciani et al., Int J Immunopathol Pharmacol. 1999, 12, 55-61) , and cutaneous manifestations of graft versus host disease (Okiyama et al., J Invest Dermatol. 2014, 134, 992-1000) are characterized by the elevation of some cytokines that signal via activation
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JAK. In this way, the compounds of the disclosure, or a pharmaceutically acceptable salt, may be able to remove the pruritus associated with cutaneous inhalation or triggered by these cytokines. In. In particular, compounds of the disclosure or a pharmaceutically acceptable salt are expected to be useful for the treatment of atopic dermatitis and other inflammatory skin diseases. In. one. aspect, therefore, the invention provides a method of treating an inflammatory skin disease in a mammal (e.g., human), the method comprising applying a pharmaceutical composition comprising a compound of the disclosure or a pharmaceutically acceptable salt and a pharmaceutical carrier on mammalian skin. In one aspect, the inflammatory skin disease is atopic dermatitis.
[00126] It has been shown that many eye diseases are associated with the elevation of pro-inflammatory cytokines that depend on the JAK-STAT pathway. The compounds of the disclosure or a pharmaceutically acceptable salt, therefore, may be useful for the treatment of various eye diseases that include, but are not limited to, uveitis, diabetic retinopathy, diabetic macular edema, dry eye disease, degeneration age-related macular disease and atopic keratoconjunctivitis. In particular, uveitis (Horai and Caspi, J Interferon Cytokine Res, 2011, 31, 733-744), diabetic retinopathy (Abcouwer, J Clin Cell Immunol, 2013, Suppl 1, 1-12), diabetic macular edema (Sohn et al ., American Journal of Opthamology, 2011, 152, 686-694), dry eye disease (Stevenson et al, Arch Ophthalmol, 2012, 130, 90-100) and age-related macular degeneration (Knickelbein et al, Int Ophthalmol Clin , 2015, 55 (3), 63-78)
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 67/165 are characterized by elevation of some proinflammatory cytokines that signal through the JAK-STAT pathway. In this way, the compounds of the disclosure, or a pharmaceutically acceptable salt, may be able to alleviate associated eye inflammation and reverse disease progression or improve symptoms. In one aspect, therefore, the invention provides one. a method of treating a disease, ocular in a mammal, the method comprising applying a pharmaceutical composition comprising a compound of the disclosure or a pharmaceutically acceptable salt and a pharmaceutical carrier for the mammalian eye. In one aspect, eye disease is uveitis, diabetic retinopathy, diabetic macular edema, dry eye disease, age-related macular degeneration and atopic keratoconjunctivitis. In one aspect, the method comprises administering the compound of the disclosure or a pharmaceutically acceptable salt by intravitreal injection. The compounds of the disclosure, or a pharmaceutically acceptable salt, can also be used in combination with one or more compounds useful for the sake of protection.
[00127] The compounds of the disclosure, or a pharmaceutically acceptable salt, may also be useful for the treatment of other diseases such as inflammatory diseases, autoimmune diseases or. cancers. The compounds of the disclosure or a pharmaceutically acceptable salt can be useful for the treatment of one or more of arthritis, rheumatoid arthritis, juvenile rheumatoid arthritis, transplant rejection, xerophthalmia, psoriatic arthritis, diabetes, insulin dependent diabetes, disease motor neuron, myelodysplastic syndrome, pain, sarcopenia, cachexia, shock
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54/13 6 septic, systemic lupus erythematosus, leukemia, chronic lymphocytic leukemia, chronic myelocltic leukemia, acute lymphoblastic leukemia, acute myelogenic leukemia, ankylosing spondylitis, myelofibrosis, lymphoma. B-cell cancer, hepatocellular carcinoma, Hodgkin's disease, breast cancer, multiple myeloma, melanoma, non-Hodgkin's lymphoma, non-small cell lung cancer, clear ovarian cell cancer, ovarian tumor, pancreatic tumor, polycythemia vera, syndrome de Sjoegrens, soft tissue sarcoma, sarcoma, splenomegaly, T-cell lymphoma and thalassemia major.
Combination therapy [00128] The compounds of the disclosure or a salt. pharmaceutically acceptable can also be used in combination with one or more agents that act by the same or different mechanisms to treat a disease. The different agents can be administered in sequence or simultaneously, in separate compositions or in the same composition. Useful classes of agents for combination therapy include, but are not limited to, a beta 2 adrenoceptor agonist, a muscarinic receptor antagonist, a. glucocorticoid agonist, an antagonist of the coupled G protein 44 receptor, a leukotriene D4 antagonist, an M3 muscarinic receptor antagonist, a histamine H1 receptor antagonist, an immunoglobulin E antagonist, a PDE 4 inhibitor, a PDE 4 antagonist IL-4, an M1 muscarinic receptor antagonist, a histamine receptor antagonist, an IL-13 antagonist, an IL ~ 5 antagonist, a 5-lipoxygenase inhibitor, a beta-adrenoceptor agonist, a CCR3 chemokine antagonist , a CFTR stimulator, an immunoglobulin modulator, a ligand inhibitor
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 69/165 interleukin 33, a PDE 3 inhibitor, a phosphoinositide-3 kinase delta inhibitor, a thromboxane A2 antagonist, an elastase inhibitor, a tyrosine kinase Kit inhibitor, a leukotriene E4 antagonist, a leukotriene antagonist, a PGD2 antagonist, a TNFalpha ligand inhibitor, a TNF binding agent, a cascade complement inhibitor, a. eotaxin ligand inhibitor, a reductant glutathione inhibitor, a histamine H4 receptor antagonist, an IL-6 antagonist, an IL2 gene stimulator, a modulator of the Fc IIB gamma immunoglobulin receptor, a. gamma interferon ligand, an interleukin 13 ligand inhibitor, a ligand inhibitor. interleukin 17 inhibitor, an L-selectin antagonist, a leukocyte elastase inhibitor, a leukotriene C4 antagonist, an inhibitor of leukotriene C4 synthase, a copper-based inhibitor of membrane amino oxidase, a. metalloprotease-12 inhibitor, metalloprotease-9 inhibitor, a mite allergen modulator, a muscarinic receptor modulator, an acetylcholine nicotinic receptor agonist, a kappa B nuclear factor inhibitor, an antagonist of. Pselectin, a PDE 5 inhibitor, one. PDGF receptor antagonist, a phosphoinositide-3 kinase inhibitor, a TLR-7 agonist, TNF antagonist, an Abl tyrosine kinase inhibitor, an acetylcholine receptor antagonist, a. mammalian acid chitinase inhibitor, an ACTH receptor agonist, an actin polymerization modulator, an Al adenosine receptor antagonist, an adenylate cyclase stimulator, an adrenoceptor antagonist, an. adrenocorticotropic hormone ligand, an alcohol dehydrogenase 5 inhibitor, an alpha 1 antitrypsin stimulator, an
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 70/165 proteinase alfa 1, nm androgen receptor modulator, a stimulator of the angiotensin-converting enzyme 2, one
agonist of ANP, a inhib-i-doj: of protein B cr, um t. agonist, a. a d r e η o c e p t o: r beta 1 antagor 1 i s t. a a d r e η o c e p beta beta 2, one modulator at < drenocep beta beta 2, a modulated beta r -amiioide an inhibitor of the gene BMP10, 1. im i n i b r r rio BMP gene i 5, one
calcium channel inhibitor, a cathepsin G inhibitor, urn. CCL26 gene inhibitor, CCR3 chemokine modulator, CCR.4 chemokine antagonist, cell adhesion molecule inhibitor, chaperonin stimulator, chitinase inhibitor, collagen I antagonist, C.3 complement inhibitor , a CSF-1 antagonist, one. chemokine antagonist CXCR2, a common beta chain modulator of the cytokine receptor, a stimulator of cytotoxic T-lymphocyte protein-4, a deoxyribonuclease I stimulator, one. deoxyribonuclease stimulator, an inhibitor of dipeptidyl peptidase I, an inhibitor of DNA gyrase, one.
DP prostanoid receptor modulator, ESelectin antagonist, EGY family tyrosine kinase receptor inhibitor, elastin modulator, ET-A endothelin antagonist, one. ET-B endothelin antagonist, epoxide hydrolase inhibitor, FGF3 receptor antagonist, Fyn tyrosine kinase inhibitor, GATA 3 transcription factor inhibitor, Glucosylceramidase modulator, glutamate receptor modulator, glutamate receptor modulator GM-CS ligand, a guanylate cyclase inhibitor, an H + K + ATPase stimulator, a hemoglobin modulator, a heparin agonist, a.
histone inhibitor deacetylase, a stimulator for histone deacetylase-2, one. HMG CoA reductase inhibitor, um. inhibitor
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37/136 of I-kappa B beta kinase, an inhibitor of the ICAM1 gene, an IL-17 antagonist, an IL-17 receptor modulator, an IL-23 antagonist, an IL-4 receptor modulator, an . an immunoglobulin G modulator, an immunoglobulin G agonist, an immunoglobulin G modulator, an epsilon Fc immunoglobulin IA receptor antagonist, an IIB antagonist immunoglobulin Fc receptor antagonist, a. kappa immunoglobulin modulator, an insulin sensitizer, a beta-interferon ligand, a similar interleukin 1 receptor antagonist, interleukin 18 ligand inhibitor, an interleukin 17A receptor inhibitor, urn. beta-interleukin-ligand inhibitor, interleukin-5 ligand inhibitor, interleukin-6 ligand inhibitor, voltage-dependent KCNA channel 3 inhibitor, kit-ligand inhibitor, laminin-5 agonist, a leukotriene receptor antagonist CysLTl, a leukotriene receptor antagonist
CysLT2, an inhibitor of the LOXL2 gene, an inhibitor of Lyn tyrosine kinase, an inhibitor of the protein MARCKS, an inhibitor of the associated protein 4 MDR, a modulator of the metalloprotease
2, one. metalloprotease-9 modulator, a mineralocorticoid receptor antagonist, an M2 muscarinic receptor antagonist, an M4 muscarinic receptor antagonist, an M5 muscarinic receptor antagonist, a natriuretic peptide receptor agonist, a natural killer cell receptor modulator , a stimulator of the alpha subunit
Nicotinic receptor ACh, a NK cell receptor modulator, um. nuclear factor kappa B modulator, one. growth factor receptor opioid agonist, a P-glycoprotein inhibitor, a P2X3 antagonist
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58/136 purinoceptor, a p38 MAP kinase inhibitor, a Peptidase 1 modulator, a phospholipase A2 inhibitor, a phospholipase C enzyme inhibitor, a plasminogen activator inhibitor 1, a platelet activating factor receptor antagonist, a PPAR gamma agonist, prostacyclin agonist, protein tyrosine kinase inhibitor, SH2 domain stimulator, inositol phosphatase, signal transduction inhibitor, one. sodium channel inhibitor, a STAT-3 modulator, a stem cell antigen-1 inhibitor, a superoxide dismutase modulator, a CD28 T cell surface glycoprotein inhibitor, a CD8 cell surface glycoprotein inhibitor T, a TGF beta-agonist inhibitor, TGF beta antagonist, a thromboxane synthetase inhibitor, a thymic stromal lymphoprotein inhibitor, a thymosin ligand inhibitor, a. beta 4 agonist thymosin, a TLR-8 agonist, a TLR-9 agonist, a TLR9 gene stimulator, a. topoisomerase IV inhibitor, a stimulator of troponin I fast skeletal muscle, a stimulator of troponin T fast skeletal muscle stimulator, an antagonist of the IL-1 receptor, a. TNF type II receptor modulator, an ion channel modulator, a uteroglobin stimulator and a VIP agonist.
[00129] Specific agents that can be used in combination with the JAK inhibitor compounds present include, but are not limited to, rosiptor acetate, umeclidinium bromide, secukinumab, methenethalin acetate, tridecactide acetate, fluticasone propionate, sulforaphane alpha- cyclodextrin-stabilized tezepelumab, mometasone furoate, thi-1467335, dupilumab,
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59/136 aclidinium, formoterol, AZD-1419, HI-1640V, rivipansel, CMP001, mannitol, ANB-020, omalizumab, tregalizumab, Mitizax, benralizumab, golimumab, roflumilast, imatinibe, REGN3500, masitinibe, apritune, , adalimumab, rupatadine, parogrelil, MK-1029, beclomethasone dipropionate, formoterol fumarate, mogamulizumab, seratrodaste, UCB-4144, nemiralisib, CK-2127107, fevipiprante, danirizine, bosentan, abataceprate, 18-papropropate, EC-papropropate, ECA , salbutamol HFA, erdosteine, PrEP-001, nedocromil, CDX-0158, salbutamol, enooosarma, R — 1PR — 022, i.enzi.lumab, fluticasone furoate, vilanterol triphenate, fluticasone propionate, salmeterol, PT-00 PRS-060, remestemcel-L, citrulline, RPC4046, nitric oxide, DS-102, gerilimzumab, Actair, fluticasone furoate, umeclidinium, vilanterol, AG-NPP709, Gamunex infliximab, Ampion, acumapimode, canakinumabe, INS-100 001, sirukumab, fluticasone propionate, mepolizumab, pitavasta trine, solithromycin, etanercept, ivacaftor, anakinra, MPC-300-IV, glycopyrronium bromide, aclidinium bromide, FP-025, risankizumab, glycopyrronium, formoterol fumarate, Adipocell, YPL-001, tiotropium bromide, biotide, tiotropium bromide, biotide indacaterol maleate andecaliximab, olodaterol, esomeprazole, mite dust vaccine, mugwort pollen allergen vaccine, vamorolone, gefapixanto, revefenacin, gefitinib, ReJoin, tipelukast, bedoradrine, SCM-CGH, RHS-602 brodalumab, BIO-11006, umeclidinium bromide, vilanterol triphenate, ipratropium bromide, tralokinumab, PUR1800, VX-561, VX-371, olopatadine, tulobuterol, formoterol fumarate, triamcinolone acetonide, xylamine
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60/136 salmeterol, fluticasone propionate, beclomethasone dipropionate, formoterol fumarate, tiotropium bromide, ligelizumab, RUTI, bertilimumab, omalizumab, glycopyrronium bromide, SENS-111, beclomethasone dipropionate, CHF5992, LT, 400F1992 glycopyrronium bromide, mometasone furoate, fexofenadine, glycopyrronium bromide, azithromycin, AZD-7594, formoterol, CHF-6001, batefenterol, OATD-01, olodaterol, CJM-112, rosiglitazone, salmeterol, setipiprante, , plecanatide, fluticasone, salmeterol, eicosapentaenoic acid monoglycerides, lebrikizumab, RG-6149, QBKPN, Mometasone, indacaterol, AZD-9898, sodium pyruvate, zileutone, CG-201, imidafenacin, CNTO-6785, CLBS-03, mom RGN-137, procaterol, formoterol, CCI-15106, POL6014, indacaterol, beclomethasone, MV-130, GC-1112,
Allergovac depot, MEDI-3506, QBW-251, ZPL-389, udenafil,
GSK-3772847, levocetirizin, AXP-1275, ADC-3680, timapiprante, abediterol, AZD-7594, ipratropium bromide, salbutamol sulphate, tadekinig alia, ACT-774312, alpha dornase, iloprene, batefenterol, flutforone, alumina ciclesonide, emeramide, arformoterol, SB-010, Ozagrel, BTT-1023, Dectrekumab, levalbuterol, pranlukast, hyaluronic acid, GSK-2292767, Formoterol, NOV-14, Lucinactant, salbutamol, prednisolone, ebastine, cipecylate, and GSx688688 , BI-443651, GSK-2256294, VR179, VR-096, hdm-ASIT +, budesonide, GSK-2245035, VTX-1463, Emedastine, dexpramipexole, levalbuterol, N-6022, dexamethasone sodium phosphate, PIN-201104, OPK 0018, TEV-48107, suplataste, BI-1060469, Gemilukast, gamma interferon, dalazatide, bilastine, fluticasone propionate, xinafoate
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61/136 salmeterol, RP-3128, bencicloquidium bromide, reslizumab, PBF-680, CRTH2 antagonist, Pranlukast, salmeterol xinafoate, fluticasone propionate, tiotropium monohydrate bromide, masilukast, RG-7990, doxofin, aboxofin, doxofol, aboxofin, doxofin, aboxofin, doxofin, aboxofin glycopyrronium, TEV-46017, ASM-024, fluticasone propionate, glycopyrronium bromide, salmeterol xinafoate, salbutamol, TA-270, Flunisolide, sodium cromoglycate, Epsi-gam, ZPL-521, salbutamol, aviptadil, TRN-157 , Zafirlukast, Stempeucel, sodium pemirolaste, nadolol, fluticasone propionate + salmeterol xinafoate, RV-1729, salbutamol sulfate, carbon dioxide + pertluorooctyl bromide, APL-1, dectrekumabe + VAK-694, acetylsalicylate, zincine TR-4, therapy of mesenchymal progenitor cells derived from human allogeneic adipose tissue, MEDI-9314, PL-3994, HMP-301, TD
5471, NKTT-120, pemirolaste, beclomethasone dipropionate, trantinterol, monosodium alfaluminol, IMD-1041, AM-211, TBS-5, ARRY-502, seratrodaste, recombinant midismase, ASM8, deflazacorte, bambuterol, RBx-10017609 fenoterol, fluticasone + formoterol, epinastine, WIN-901X, VALERGEN-DS, OligoG-COPD-5/20, tulobuterol, oxis Turbuhaler, DSP-3025, ASM-024, mizolastine, budesonide + salmeterol, LH011, AXP-E, immunoglobulin human histamine, YHD-001, theophylline, ambroxol + erdosteine, ramatroban, montelukast, pranlukast, AG ~ 1321001, tulobuterol, ipratropium + salbutamol, tranylast, methylprednisolone suleptanate, colforsine daropate, repirinaste and repirinaste.
[00130] Here also one is provided composition pharmaceutical • eendend ' o a compound of disclosure or u acceptable p salt ionto pharmaceutical view ico and one or more
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62/136 therapeutic agents. The therapeutic agent can be selected from the class of agents specified above and from the list of specific agent described above. In some embodiments, the pharmaceutical composition is suitable for delivery to the lungs. In some embodiments, the pharmaceutical composition of the invention is suitable for inhalation or nebulization administration. In some embodiments, the pharmaceutical composition is a dry powder or liquid composition.
[00131] Furthermore, in one aspect of the method, the invention provides a method for treating a disease, or disorder in one. mammal comprising administering to the mammal a. compound of the disclosure or a pharmaceutically acceptable salt and one or more therapeutic agents.
[00132] When used in combination therapy, the agents can be formulated in a single pharmaceutical composition, or the agents can be supplied in. different compositions that are administered simultaneously or at different times, by the same route or by different routes of administration. Such compositions can be packaged separately or can be packaged as a kit. The two or more therapeutic agents in the kit can be administered by the same route of administration or by different routes of administration.
[00133] The compounds of the invention have been shown to be potent inhibitors of the enzymes JAK1, JAK2, JAK3 and TYK2 in enzyme binding assays and have potent functional activity without cytotoxicity in cell assays, and exert the pharmacodynamic effects of inhibiting JAK in models preclinical, as described in the following examples.
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EXAMPLES
[00134] The following synthetic and biological examples are Hi. Θ 'C Θ C í. d-O 8 p '1 illustrate the invention, and are not to be interpreted n in any way as a limitation of the scope of invention · Nos examples below, abbreviations have the following Meanings, unless otherwise stated. Abbreviations right defined below have their accepted meanings ACN ^: == acetonitrile DCMDIPEA ^::: dichloromethane = N _r N- di - is op rop ieti 1 ami na DMF = N, .íV “dimethylformam.ida EtCAc == ^: ethyl acetate 1t = hour (s) HATU = Ν, Ν, Ν ’, M'-tetram.ethyl hexafluorophosphate ·0- (7-azabenzotriazol-l-yl) uronium IPAI PAc = isopropyl alcohol == ^: isopropyl acetate Me OH ^: == methanol min. = min.in (s) Pd (PPh ₃ ) 4 = Tetrakis (triphenylphosphine) palladium (0) OK = room temperature TEATHE ^::: trifluoroacetic acid = tetrahydrofuran
bis (pinacolato) diboron = 4,4,5, 5, 4 ', 4', 5 ', 5'-octamethyl [2,2'] bi [[1,3,2] dioxaborolanil] [00135] The reagents and solvents were purchased from commercial suppliers (Aldrich, Fluka, Sigma, etc.), and used without purification. The progress of the reaction mixture was monitored by thin layer chromatography (TLC), high performance liquid chromatography (HPLC / HPLC)
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64/136 analytical) and mass spectrometry. The reaction mixtures were worked out as described specifically in each reaction; were, purified by extraction and other purification methods, such as crystallization and precipitation dependent on temperature and solvent. In addition, the reaction mixtures were purified by column chromatography or preparative HPLC, generally using Cl8 or column BDS packaging, and conventional eluents. Typical conditions for preparative HPLC are described below.
[00136] The characterization of the reaction products was made, using mass spectrometry and ^ H-NMR. For NMR analysis, the samples were dissolved in deuterated solvents (such as CD ₃ OD, CDCI3, or d ₆ ~ DMSO), and the NMR spectra were acquired with the Varian Gemini 2000 (400 MHz) instrument under observation conditions standards. The identification by mass spectrometry of the compounds was done by a. electrospray ionization method (ESMS) with. an instrument from Applied Biosystems, (Foster City CA) model API 150 EX or an instrument from Waters (Milford, MA)
3100, coupled with self-purification systems.
Preparative HPLC conditions
Column: Cl8, 5 pm. 21.2 x 150 mm or Cl8, 5 pm21 x 250 or Cl4, 5 pm 21x150 mm Column temperature: T emp e r a. t u r amb i e n t e Flow rate: 20.0 ml / min. Mobile phases: A == Water + 0.05% TEAB == ACN + 0.05% TEA, Injection volume: (100-1500 pl)
Wavelength of the 214 nm detector:
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65/136 [00137] The crude compounds were dissolved in 1: 1 of water: acetic acid in about 50 mg / ml. A 4 minute run of the test analytical scale was performed using a 2.1 x 50 mm C18 column, followed by a 15 or 20 minute preparative scale run using 100 pL injection with the gradient based on% B retention. run of the test analytical scale. The precise gradients depended on the sample. Samples with impurities that had close retention times were checked with a 018 21 x column
250 mm and / or C14 column 21 x 150 mm for better separation. Fractions with the desired product were identified by mass spectrometry analysis [00138] In the following synthetic examples, compounds with numbers less than 20 refer to intermediates shown in Schemes 1 to 4 where the prime designates a compound with . a particular choice of the protection group.
Preparation 1: 2 ~ (4- (Benzyloxy) - ~ 2 ~ ethyl ~ 5 ~ fluorophenyl) 4,4,5,5-tame 1-1,3,2-d.ioxaborolane (9)

(a) 1- (Benzyloxy) -4-bromo-5-ethyl-2-fluorobenzene (21) [00139] To a solution of 4-bromo-5-ethyl-2-fluorophenol (20) (20 g, 910, 32 mmol) in ACN (250 ml) K2CO3 (31.55 g, 228.3 mmol) was added followed by benzyl bromide (13.10 ml, 109.58 mmol) dropwise. The resulting reaction mixture was stirred at 80 ° C for 2 h. The aqueous layer
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 80/165 ό 1 6 was extracted with EtOAc (three times), combined and washed with brine. The organic layer was dried over Na ₂ SO4 and evaporated under reduced pressure to achieve the intermediate title as a light yellow oily liquid (25 g, 89% yield). ¹ H NMR (400 MHz, chloroform-d) δ 7.48 7.3 0 (m, 5H), 7.2 7 (d, J -------- 10.5 Hz, 1H), 6 , 8 7 (d, J ------- 8.7 H z, 1H), 5, 12 (s, 2 H), 2.66 (q, <7 = 7, b H z, 2 H ), 1, 16 (c, J = 7.5 Hz, 3H).
(b) 2- (4- (Benzyloxy) -2-ethyl-5-fluorophenyl) -4,4,5,5tetramethyl-1,3,2-diozaborolane (9) [00140] To a solution of the product from the previous step (21) (12.5 g, 40, 45 mmol) in. dioxa.no (100 ml) (bis) pinacolate diboron (15.40 g, 60.67 mmol) and KOAc (11.9 g, 121.35 mmol) were added. The reaction mixture was purged with nitrogen for 15 min. followed by addition of [l, l ^f bis (diphenylphosphino) ferrocene] dichloropalladium (II) complex. dichloromethane (1.65 g, 2.023 mmol). The reaction mixture was stirred and heated to 110 ° C for 3 h, filtered through Celite and the residue washed with EtOAc. The filtrate was diluted with excess EtOAc (200 ml) and washed with. water (100 ml) followed by brine (100 ml), dried over sodium sulfate and concentrated in vacuo to obtain the crude product which was purified by column chromatography on (100200) silica gel, eluted with. 3-5% EtOAc: hexane to obtain the desired product as one. almost white solid (9.50 g, 66% yield). NMR MI (400 MHz, chloroform-d) δ 7.54 - 7.27 (m, 6H), 6.81 (d, J ------- 7.9 Hz, 1H), 5.16 ( s, 2H), 2.84 (q, J ----- 7.5 Hz, 2H), 1.32 (s, 12H), 1.14 (t, J = 7.5 Hz, 3H).
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Preparation 2: 6- (4- (benzyloxy) "2-ethyl" 5 ~ fluorophenyl) -1 (tetrahydrO "2H" pyran-2 "yl) -3- (trimethylstannyl) -IH-indazole (3 ')
(a) 6- (4- (Benzyloxy) -2-ethyl-5-fluorophenyl) -1 (tetrahydro-2H-pyran-2-yl) -177-indazole (22) [00141] To a 6-bromine solution -l- (tetrahydro-2H-pyran2-yl) -IH-indazole (10) (50 g, 178.57 iranol) and 2- (4- (benzyloxy) 2-ethyl - 5-fluorophenyl) -4 , 4,5,5-tetramethyl-1,2,2-dioxaborolane (9) (76.3 g, 214.29 mmol) in DMFmUO (480: 120 ml) K ₃ PO ₄ (94.64 g, 446.86 mmol). The reaction mixture was degassed with nitrogen for 15 minutes and then the catalyst Pd (PPh ₃ ) ₂ Cl ₂ (6.26 g, 8.93 mmole) was added and the mixture was again degassed with nitrogen for 5 minutes and heated to 100-110 ° C for 5 hours. The reaction mixture was filtered through Celite and the residue was washed with EtOAc. The filtrate was diluted with EtOAc, washed with cold water and brine, dried over sodium sulfate and concentrated in vacuo to provide crude product which was purified by chromatography. flash column for
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68/136 to obtain the title intermediate as a white solid (65 g,
86% and yield). (m / z): [M + H] ⁺ calculated for 431.21 of
C27H27 -FN ₂ O ₂ was found 431.46. NMR ~ H (4C ) 0 MH z, chloroform d) δ 8.06 - 7 / 98 (i n, 2H), /, ’0 (Ο, J - 8.2 H z _s 1H), 7.51 - 7.3 2 (m, 5H), 7.08 (dd, J ------ 809, 6, 8.3 Hz, 1H), 7.03 (d, J ------- 11.9 Hz, 1H), 6 _Λ 95 (d, J ----- 8, 5 Hz, 1H), 5, 7 6 - 5.64 (m, 1H), 5.20 (s, 2H), 4.04 (d, J = 10.1 Hz, 1H), 3 , 72 ( _L C, J = 9, IH z ,, 1H), 2.0 2,, q, J ~ ' 7.5 Hz, 2H), 2.22 - 2.02 (m, 3H), 1.8 0 -
1.71 (m, 3H), 1.0 6 (t, J = 7.5 Hz, 3H).
(b) 6- (4- (Benzyloxy) -2-ethyl-5-fluorophenyl) -IH-indazole (23) [001421 To a solution of the product from the previous step (22) (65 g, 151.16 mmol) in methanol (700 ml) was added
HCL conc. (120 ml) and the resulting solution was heated to 6065 ° C for 3 h, cooled to RT and concentrated in vacuo. The residue was dissolved in EtOAc and washed with saturated NaHCCu solution and water. The organic layer was dried over anhydrous Na2SC'4 and concentrated in vacuo to achieve the intermediate title as a white solid (52 g, 99% (crude)). ^X H NMR (400 MHz, chloroform-d) δ 8.13 (s, 1H), 7.7 7 (d, J = 8.3 Hz, 1H), 7.59 - 7.30 (m, 6H ), 7.10 (d, J = 8.3 Hz, 1H), 7.01 (d, J - = 11.8 Hz, 1H), 6.9 6 (d, J ------ - 8.4 Hz, 1H), 5.21 (s, 2H), 2.53 (q, J ------- 7.5 Hz, 2H), 1.05 (t, J - ----- 7.5 Hz, 3H).
(c) 6- (4- (Benzyloxy) -2-ethyl-5-fluorophenyl) -3-iodo-1H-indazole (24)
[00143] To a 6- solution (4- (benzyloxy) -2-ethyl-5- fluorophenyl) -IH-indazole (23) (56 g, 161.18 mmol) in DMF (400 ml) KOH (36, ( 2. g, 647.39 mmo1) and mixture was stirred for 5,000 luLos. ./the iodine solution
(82.2 g, 323, 69 mmol) in. DMF (100 ml) was added
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69/136 slowly at 0 ° C and stirred at RT for 30 minutes, diluted with water (3 x 150 ml) and extracted with. EtOAc (3 x 200 ml). The organic layer was washed with. saturated aqueous sodium metabisulfite solution (3 x 200 ml) and water (400 ml), dried in anhydrous NazSCq and concentrated under reduced pressure to obtain the crude product which was purified by flash column chromatography to obtain the intermediate title as a .
brownish semi-solid (64 g, 84% yield). NMR • 'Ή (400 MHz, chloroform-d) δ 10.49 (s, 1H), 7.57 - 7.32 (m, 7H), 7.16 (d, J 8.3 Hz, 1H), 7.04 - 6.91 (m, 2H), 5.2 0 (s 2H), 2.51 (q, J = 7.4 Hz, 2H), 1.04 (t, J = 7.5 Hz , 3H).
(d) 6- (4- (Benzyloxy) -2-ethyl-5-fluorophenyl) -3-iodo-1- (tetrahydro-2H-pyran-2-yl) -lif-indazole (25) [00144] cold solution of the product from the previous step (24) (60 g, 127.12 mmol) in DCM (700 ml) p-toluenesulfonic acid (4.84 g, 25.423 mmol) was added followed by 3,4-dihydro-2. H-pyran (17.4.3 ml, 190, 68 mmol) dropwise. The reaction mixture was stirred at RT overnight, diluted with DCM and washed with saturated aqueous NaHCO3 solution and brine. The organic layer was dried over anhydrous Na2SC'4 and concentrated under reduced pressure to provide crude product which was purified by flash chromatography (silica gel) to obtain the intermediate title as an off-white solid (64 g, 91% yield).
(m / z): [M-1-H] calculated for 557 of C27H26FIN2O2 was found 557.30. NMR ‘Ή (400 MHz, chloroform-d) δ
7.56 7.31 (m, 7H), 7.14 (d, J
Hz, 1H), 7.01 (d, J ------ 11, 8
Hz, 1H), 6.95 (d, J = 8.5 Hz, 1H), 5.68 (d, J
9.3 Hz, 1H), (s, 2H),
4.08
3.99 (m, 1H), 3.77 (m, 1H), 2.50
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70/136 (q, J = 7.2 Hz, 2H), 2.23 - 1.97 (m, 3H), 1.81 - 1.68 (m, 3H), 1.06 (t, J = 7.4 Hz, 3H).
(e) 6- (4- (benzyloxy) -2-ethyl-5-fluorophenyl) -1- (2H-pyran-2-yl) -3- (trimethylstannyl) -IH-indazole (3 ') [00145] To a solution of 6- (4- (benzyloxy) -2-ethyl-5 fluorophenyl) -3-iodo-1- (tetrahydro-2B-pyran-2-yl) -IJf-indazole (25) (20 g, 35 , 97 mmol) in toluene (150 ml) hexamethyldithine (9.2 ml, 43.17 mmol) was added. The reaction mixture was degassed with nitrogen for 20 minutes, followed by the addition of tetrakis (2.0 g, 1.80 mmol) and then stirred at 100 ° C for 2 h, cooled to RT, filtered through CELITE and the residue. washed with. EtCAc. The filtrate was concentrated and purified by column chromatography (neutral alumina), eluted with 2-5% EtOAc: hexane to obtain the compound title (17.50 g, 82% yield).
(in / z): (M + Hj calculated for 557.10 C27H26I '1N2O2 was found 557.30. (m / z): [M + H]' calculated for 595.17 for C30H35FN2O2, 593, 17 was MHz , chloroform-d) δ (m, 6H), 7.13 - 7.00
- 5.68 (m, 1H), 5.21
3.66 (m, 1H), 2.54 (q, J -------- 7.3 Hz, 2H), 2.23 - 2.00 (m, 2H 1, 8 7 - 1, 59 (m, 4H), 1.0 8 (t, 7.5 Hz, 3H), 0.47 (s, 9H found 595.49, 593.5o. NMR Ή (4
7.68 (d, J -------- 8.0 Hz, 1H), 7.57 - 7.2 (m, 2 H), 6.96 (d, J = 8.4 Η z , 1H), 5, (s. 2H). 4.13 - 4.0 0 (m. 1H), 3.81 Attorney's Tariff No .: P-338-PCT
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Preparation 3: 5- (tert-butyl) 6 ~ methyl (S) ~ 2 ~ iodine ~ 3 ~ ((2 trimethylsilyl) ethoxy) methyl) - 3,4,6,7-tetrahydro -5Himidazo [ 4,5-c] pyridine-5,6-diaarboxylate (4 ')
(a) (S ') acid - 4,5,6,7-tetrahydro-3H-imidazo [4,5
c] pyridine-6-carboxylic (11) [001461 To a stirred suspension of L-histidine (26) (50 g, 322.24 mmol) in. water (420 ml) conc.
(29 ml) dropwise at 0 ° C, followed by formaldehyde (55 ml, 676.72 mmol) in one part at 0 ° C. The reaction mixture was stirred for 30 minutes and then heated at ° C for 6 h and concentrated. The resulting crude product was stirred for 2 h with. diethyl ether, filtered and washed with 1PA: THF (100: 300 ml) to provide the hydrochloride salt of the intermediate title as an off-white solid (75 g, 99% yield (crude)), (m / z): [M + Hb calculated for 168, 07 of C7H9N3O2 was. found 168.17.
(b) Methyl (S) -4,5,6,7-tetrahydro-3H-imidazo [4,5-
c] pyridine-6-carboxylate (27) [00147] To a stirred solution of the product from the previous step (11) (75.0 g, 312.5 mmol) in methanol (1500 ml) was added SOCI2 (45.6 ml , 625 mmol) dropwise at 0 ° C and stirred at RT for 16 h and then heated under reflux (70 ° C) for 1 hour. The solvent was removed by
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Petition 870190088769, of 09/09/2019, p. 86/165 distillation and the crude product was triturated with methanol followed by diethyl ether to provide the hydrochloride salt of the intermediate title as an off-white solid (80 g crude). ⁹ H NMR (400 MHz, DMSO ~ d ₆ ) O 9.05 (s, 1H), 4.71 (dd, J ------- 9.4, 5.2 Hz, 1H), 4 , 3 6 (d, J - = 15.5 Hz, 1H), 4.3 0 (d, J ------- 15.6 Hz, 1H), 3.82 (s, 3H), 3.4 4 - 3.21 (m, 2H).
(c) 5- (tert-Butyl) 6-methyl (S) -3, 4, 6, 7-tetrahydro-5Himidazo [4,5-cj pyridine-5,6-dicarboxylate (28) [00148] To a solution stirring of the product from the previous step (27) (80.0 g, 314.96 mmol) in methanol (1000 ml) was added DIPEA (282 ml, 1574 mmol) followed by di-tert-butyl dicarbonate (172 ml, 787 , 48 mmol) at 0 ° C. The reaction mixture was stirred at RT for 16 h and then liquid NH ₃ (150 ml, 25% in water) was added and the reaction mixture was stirred again at 16 RT, methanol was distilled off and the residue was extracted into DCM (3 x 200 ml). The combined organic extracts were dried over anhydrous Na2SO ₄ , concentrated and purified by flash chromatography (100-200 mesh silica gel), eluted with 5% MeOH: DCM to obtain the intermediate title (41 g, 46% Yield). ijn / z): [M + H] 'calculated for 282.14 of C13H19N3O4 fol found <282.21. NMR 'Ή (400 MHz, EMSO-de) δ 11.85 (s, 1H), 7.50 (s, 1H), 5.18 (dd, J ------ 49, 3, 5, 1 Hz, 1H), 4.51 (t, J --- 14.2 Hz, 1H), 4.09 (dd, J = 4 3.9, 16.1 Hz, 1H), 3.59 (s , 3H), 3.08 (d, J = 15.5 Hz, 1H), 2.94 (d, J = 15.1 Hz, 1H), 1.45 (s, 9H).
(d) 5- (tert-Butyl) 6-methyl (S ') - 2-iodo-3, 4, 6, 7tetrahydro-5n-imidazo [4,5-c] pyridine-5,6-dicarboxylate (29) [00149] To a solution of the product from the previous step (29) (41.0 g, 145.9 mmol) in THE (500 ml) was added Niosuccinimide (66.0 g, 291.8 mmol) at 0 ° C and the solution
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Petition 870190088769, of 09/09/2019, p. The resulting 87/165 was stirred at RT for 4 h, diluted with water and extracted with ethyl acetate. The organic portion was washed with solution and 10% sodium thiosulfate (3 x 2u0 ml). The combined organic layer was dried over anhydrous sodium sulfate and concentrated to provide the compound title 60 g (crude), which was used in the next step without purification, (m / z): [M + H] ⁺ calculated for 408.31 of C13H18IN3O4 was found 408.03. NMR • 'Ή (400 MHz, DMSO-ah) δ 12.48 (s, 1Η), 5, 34 - 4.97 (m, 1H), 4, 67 - 4.35 (m, 1H), 4, 12 - 3.95 (m, 1H), 3.60 (s, 3H), 3.14 - 2.82 (m, 2H), 1.44 (s, 9H).
(e) 5- (tert-Butyl) 6-methyl (S) -2-iodo-3 - (((2trim.ethylsilyl) ethoxy) methyl) - 3,4, 6, 7 ~ tetra.id.ro - 5Himidazo [4,5-c] pyridine-5,6-tip.rboxy.late (4 ') [00150] To a stirred solution of 5- (tert-butyl) 6methyl (S) -2-iodine-3, 4 , 5,7-tetrahydro-5.H-imidazo [4,5c] pyridine-5,6-dicarboxylate (29) (40 g, 0.098 mol) in DMF (150 ml) was added DIPEA. (35.1 ml, 0.19 mol) a. 0 ° C. The reaction mixture was stirred for 10 minutes, then 2- (trimethylsilyl) -ethoxymethyl chloride (19.1 ml, 0.10 mol) was added dropwise at 0 ° C. The reaction mixture was stirred for 3 h at RT. After 4 h, chilled water was added and the reaction mixture was extracted with EtOAc (2 x 200 ml). The organic layer was dried over anhydrous sodium sulfate, concentrated and purified by chromatography on. column, flash, eluted with. hexane: EtOAc
20-35%, to obtain the product title as a light yellow viscous liquid (27 g). (m / z): [M + H] ⁺ calculated for
8.12 of C19H32IN.3O5SÍ was found 538.42. 1 H NMR (400
MHz, DMaO-d _s ) δ 5, 33 - 5, 04 (m, 3H), 4.79 - 4.56 (m, 1H),
4.54 - 4.14 (m, 1H), 3.60 (s, 3H), 3.47 (t, J = 7.8 Hz,
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3.31 - 3.16 (m, 1H), 2.97 (t, J == 18.9 Hz, 1H), 1.44 is, 9H), 0.92 - 0.74 (m, 2H) , -0.03 (s, 9H).
Preparation 4: (6S) -5- (tert-butoxycarbonyl) -2- (6 (2-ethyl-5 ”fluoro-4“ hydroxyphenyl) -1- (tetrahydro-2H-pyran-2-yl) 1H-indazole -3-yl) -3 - ((2- (trimethylsilyl) ethoxy) methyl) -4,5,6,7 tetrahydro ~ 3H ~ imidazo [4,5-c] pyridine-6 ~ carboxylic (7 ')
F
HP SEM
6 ’
(a) 5- (tert-Butyl) 6-methyl (6S) -2- (6- (4- (benzyloxy) -2 ethyl-5-fluorophenyl) -1- (tetrahydro-2H-pyran-2-yl) -177-indazole-
3-yl) -3- ((2- (trimethylsilyl) ethoxy) methyl) -3,4,6,7-tetraic
5.H-imidazo [4,5-c] pyridine-.b, 6-dicarboxylate (5 ') [001511 To a stirred solution of 5- (tert-butyl) 6methyl (S) -2-iodo-3- ( (2-trimethylsilyl) ethoxy) methyl) - 3,4,6,7 tetrahydro -5H-imidazo [4,5-c] pyridine-5,6-dicarboxylate (4 ') (17.0 g, 31.65 mmol ) in toluene (500 ml) was added 6 (4- (benzyloxy) -2-ethyl-5-fluorophenyl) -1- (tetrahydro-2.H-pyran2-yl) -3- (trimethylestanill) -IH-indazole ( 3 ') (20 g,
34.82 mmol). The reaction mixture was purged with argon
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3.16 mmol) and copper iodide (1.20 g, 6.33 mmol) and the reaction mixture was stirred at 120 ° C for 16 h. The reaction mixture was filtered through Celite, the filtrate was concentrated under reduced pressure and purified by silica gel column chromatography (Redisep column 80 g, eluted with DCM for 10 minutes and then in. 15-20% EtOAc in. hexane to obtain the intermediate title as a yellow solid (15.10 g, 58% yield). (m / z): [M + H] ⁺ calculated for 840, 41 for C46H58FN5O7SI 840.54 was found.
² N NMR ( 4 00 MH z, Cloi(S, 1H ofórj), Ί, mio-d) δ 805 (d, J = , 43: 11, (s, 1H),4 Hz, 1H I _f 5 4) f ° f - 7.339 5 (d, J (m, 6H), ==: 8, 5 Hz , 1H), 6, 0 9 The f Ό 9 (m, 3H), The f o 9 - 5.36 (m, 1H), 5.20 (s, 2H), 4.97 - 4, 8 0 (m, 1H), 4, 12 - 3, 90 (w 1 H), 3.68 f 3H), 3.57 - 3.47 (n't, 2H), 3.40 (d, 1H), 3.2 -í __ 3 , 05 (m, 1H), z, / 4 - 2, 34 (m, 4H), 2.25 - 2, 07 (: m, 2H), 1.94 - 1.65 (m, 4H), 1.54 (S, 9 H ),% 12 - 0.99 (m, 3H), 0.9 1 - 0 , 75 (m, 2H), -0, 12 (s, 9H).
(b) 6-Benzyl 5- (tert-butyl) (6S) -2- (6- (4- (benzyloxy) -2ethyl-5-fluorophenyl) -1- (tetrahydro-2H-pyran-2-yl) - IFf-indazol3-11) -3- ((2- (trimethylsilyl) ethoxy) methyl) -3,4,6,7-tetrahydro5.H-imidazo [4,5-c] pyridine-5,6-dicarboxylate (6 ') [00152] To a round bottom flask the product from the previous step (5') (15.0 g, 17.85 mmol) in toluene (400 ml), benzyl alcohol (46.3 ml) and Ti (OEt) 4 (7.15 ml, 35.70 mmol) and the reaction mixture was refluxed
vigorous amente (140 o _r , v, i during 48 h with water and extracted with DCM. The suspension was fil trad . i, the filti 'ado ίο.' . dried on In ₂ SO ₄ , c o n c e n t r a d a. UNDER pressure reduced and puri stayed by
chromatography on. column of silica gel (column Redisep 80, 0Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 90/165
5% EtOAc in hexanes) for 20 minutes to remove the
excess ο ct and 1 c o ' ol benzyl So eluted with. 10-15% EtOAc in. hexane ) for fo rename the title .ilo in termediary. H ¹ NMR is in con formity with the strui üura. (m / z): [M + H] ⁺ calculated
for 916.44 of C52H62FN5O7SI 916.86 was found.
(c) (6S) -5- (tert-butoxycarbonyl) -2- (6- (2-ethyl-5-fluoro-4-hydroxyphenyl) -1- (tetrahydro-2.H-pyran-2-yl) - 1Hindazol-3-yl) -3 - ((2- (trimethylsilyl) ethoxy) methyl) -4,5,6,7tetrahydro-3H-imidazo [4,5-c] pyridine-6-carboxylic (7 ') [00153 ] To a stirred solution of the product from the previous step (6) (21.0 g, 22.92 mmol) in 1: 1: IPA THE (400 ml)) was added Pd (OH) 2 (5.0 g). The reaction mixture was stirred at RT for 16 h under a hydrogen flask, filtered through Celite, concentrated under reduced pressure and purified by silica gel column chromatography (column 80 Redisep, eluted with. 25-40% EtOAc in. hexane) to provide the compound title (6.1 g, 8.29 mmol) as an off-white solid). (m / z): [M + H] ⁺ calculated for 736.35 of C38H50FN5O7SÍ 736, 5 was found. 5. The H-NMR complies with. the structure, (zn / z): [M + H] ⁺ calculated for 736, 35 of C38H50FN5O7SÍ was found 736, 5. NMR ^] Ή (400 MHz,
DM SO - in ) δ 12.94 S ! 1H), 9.86 (s. 1H), 8, , 34 L; < J ^: == 7 , 6 Hz. 1H), 'f 6 6 (s, 1H ), 7 , 20 (d, J - 8, 7 H z, 1 H) _f 7, 03 (d, J 1 1 H H '6 9 3 ( (Ί. 7 = C) ii m £ 1 'i ~ 5 77 (rrJ. .1. ff -ί- Zx f .J f .1.-u J7J. ; f , -L -c1. f 11} 5, 33 ... 5.0 6 (m, 1H), 4.87 - 4, 5 6 ( m, 1H),2 - 4.14 (m, 1H), 3; 9 7 - 3.69 (m, 2H), 3.53 --- 3 , 40 (m. 2H) , 3, 2 3 --- 3.11 (m, 1H) Λ 3 , 11 - 2.93 (m, 1H), 2.47 - 2.44 (m, 2H) , 2.1 3 ~ 1.9 6 (11J ., 2 Η), 1, 68 ( d, <7 = / 0, 9 Hz , 4H), 1.4 8 s, 9H), 1.02
(t, J ~ 7, b Hz, 3H), 0.8 - 6, 0.68 (m, 2H), -0.17 (s, 9H).
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 91/165
77/136
Preparation 5: (S) ~ 2 ~ (6 ~ (2 ~ ethyl ~ 5 ~ fluoro ~ 4 ~ hydroxyphenyl) -1H-indazol-3-yl) -4,5,6,7-tetrahydro ~ 3Jfimidazo [4, 5-c] pyridine-6-carboxylic (8 ')
[001541 To a stirred solution of (6S) -5- (tert butoxycarbonyl) -2- (6- (2-ethyl-5-fluoro-4-hydroxyphenyl) -1 (tetrahydro-2A-pyran-2-yl) -1H-indazol-3-yl) -3- ((2 (trimethylsilyl ethoxy) -methyl) -4,5,6,7-tetrahydro-3Himidazo [4,5-c] pyridine-6-carboxylic (7 ') (5.7 g, 7.75 mmol) in 5: 1 dioxane: water. (60 ml) HCl conc (20 ml) was added dropwise at 0 ° C. The reaction mixture was heated and stirred at 90 ° C for 16 h and vacuum distilled to provide the crude residue, which was triturated in sequence with diethyl ether and acetonitrile to provide the hydrochloride salt of the compound title (3.6 g, 95% yield) as a light brown solid. (m / z): [M + H] ⁺ calculated for 422.16 of C22H20IN50 / 3 roi found 422.24. NMR ~ u 1,400 Mhz, D ₂ 0 / DMSO— q ' _õ ) δ 8.22 (d, J = 8.4 Hz, 1H), 7.49 (s, 1H), 7.19 (d, J = 8.1 H z, 1 H), 6.99 (d, J = 11.9 Η z , 1 H), 6.91 (d, J = 9, 0
Hz, 1H), 4.56 - 4.51 (m, 1H),
4.3 6 (d, J == 15, 5 Hz, 1H),
4.30 (d, J = 15.5 Hz, 1H), 3.35
3.25 1, m, 1H), 3, 15 - 3.05 (m, 1H), 2.4 - 2.55 (m, 2H), 0.97 (t, d = 7.5 Hz, 3H).
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 92/165
78/136
Preparation 6: (S) ~ 2 ~ (6 ~ (2 ~ ethyl ~ 5 ~ fluoro ~ 4 ~ hydroxyphenyl) "1H-indazol-3-yl) -5 ~ propyl - 4,5,6,7-t .etraidro3H “imidazo [4,5 ~ -c] pyridine-6-carboxylic
[00155] To a solution of (S) -2- (6- (2 ~ ethyl ~ 5 ~ fluoro-4-hydroxyphenyl) -1H-indazol-3-yl) -4,5,6,7-tetrahydro3H ~ imidazo [4,5 ~ c] pyridine-6 "carboxylic acid, HCl (400 mg,
0.874 mmol) (8 ') and propionaldehyde (0.095 ml, 1.310 mmol) in DMF (7 ml) was added sodium cyanoborohydride (165 mg, 2.62 mmol) and the mixture was added. reaction was stirred at RT overnight. Sodium borohydride (33 mg, 0.874 mmol) was added, the solution was concentrated and purified by preparative HPLC to provide the compound title TFA salt (179 mg, 37% yield). (m / z): [M + H] ⁺ calculated for 4 64.2 0 of C25H26FN5O3 4 64.5 was found.
Preparation 7: (JJ) ~ 2 - (6 - (2 ~ ethyl - 5 ~ fluoro ~ 4 ~ hydroxyphenyl) -IH-indazol-S-yl) ~ 5 ~ isopropyl ~ 4,5,6 , 7tetrahydrO “3H“ imidazo [4,5-a] pyridine-6-carboxylic
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 93/165
79/136
0.874 mmol), acetone (0.192 ml, 2.62 mmol) and acetic acid
(0.150 ml, 2.62 mmc ) 1) on. DMF (7 ml) was a cl i c i n. a d o C .1. cl Γ10 O O -C 0.1. ( ire to de sodium (274 mg, 4.37 ra • mol) and the mist ura da reaction was agitated the TA d howling at night. 0 borohydride in
sodium (33 mg, 0.874 mmol) was added, the solution was concentrated and purified by preparative HPLC to provide the compound title TFA salt (115 mg, 23% yield). (m / z): [M + H] 'calculated for 464.20 of C25H26FN5O3 4 64.5 was found.
Preparation 8: (S) -2- (6- (2 ~ ethyl ~ 5 ~ fluoro ~ 4 ~ hydroxyphenyl) -IH-indazol-S-yl) ~ 5-: methyl ~ 4,5,6,7 ~ tetrahydro acid -3Jí “imidazo [4,5-c] pyridine-6-carboxylic
fluoro-4-hydroxyphenyl) -1H-indazol-3-yl) -4,5,6,7-tetrahydro
3ifimidazo [4,5-c] pyridine-6-carboxylic, HCl (8 ’) (300 mg,
0.655 mmol) and formaldehyde 37% by weight in water (0.059 ml,
0.786 mmol) DMF (5 ml) was added sodium cyanoborohydride (165 mg, 2.62 mmol) and the reaction mixture was stirred at RT overnight. Sodium borohydride · (25 mg,
0.655 mmol) was added, the solution was concentrated and purified by flash chromatography (100 g column, 575% ACN / water), to provide the compound title TFA salt (85 mg, 24% yield). (m / z): [M + H] ⁺ calculated for 4 3 6, 17 of C23H22FN5O3 was found 436.45.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 94/165
80/136
Preparation 9: (S) -5-Ethi1-2- (6- (2-ethyl ”5-fluoro ~ 4hydroxyphenyl) -HH-indazol-3-yl) -4,5,6,7-tetrahydro ~ 3Jfimidazo [ 4,5-c] pyridine-6-carboxylic
[00158] To a solution of (S) -2- (6- (2-ethyl-5fluoro-4-hydroxyphenyl) -1H-indazol-3-yl) -4,5,6,7-tetrahydro3H-imidazo [ 4,5-c] pyridine-6-carboxylic, HCl (8 ') (450 mg, 0.983 mmol) and acetalcieido (0.083 ml, 1.474 mmol) in DMF (7 ml) was added cyanoborohydride sodium (247 mg, 3.93 mmol) and the mixture of. reaction was stirred at RT overnight. Sodium borohydride (112 mg, 2.95 mmol) was added, the solution was concentrated, dissolved in 1: 1 acetic acid: water + 300μ1 TEA (7 ml) and purified by chromatography on. flash (100 g column, 5-65% ACN / water) to provide the compound title TFA salt (165 mg, 0.293 mmol, 30% yield). (m / z): [M + H] ⁺ calculated for 450, 19 of C24H24FN5O3 was found 450.
Example 1: ((S) -2- (6- (2 "ethyl ~ 5 ~ fluoro ~ 4" hydroxyphenyl) 1 / f-indazol-3-yl) -5-propyl-4,5,6,7-tetrahydro -3H-imidazo [4,5cJpiridin-6-yl) ((1S, 4S) -5-methyl-2,5-diazabicyclo [2.2.1] heptan-2-yl) methanone
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 95/165
81/136 [00159] To a solution of (S) -2- (6- (2-ethyl-5fluoro-4-hydroxyphenyl) -1H-indazol-3-yl) -5-propyl-4,5,6 , 7tetrahydro-3-imidazo [4,5-c] pyridine-6-carboxylic, TEA (30 mg, 0.052 mmol), (1S, 4S ') - 5-methyl-2,5-diazabicyclo dihydrobromide [2.2.1] heptane (42.7 mg, 0.156 mmol), and DIPEA (0.064 ml, 0.364 mmol) in DMF (1.5 ml) was added HATU (29.6 mg, 0.078 mmol) and the reaction mixture was stirred at RT for at night. Hydrazine (5 eq) was added, the reaction mixture was concentrated and purified by preparative HPLC to provide the TEA salt of the compound title (27 mg, 66%
heat T. endiment) . (ffi / z): [M , + Hj ⁺ calculated for. 558.29 of 31- ⁿ 3 eFNvCb was found 3 5 or 3 e ⁵ H NMR (4 00 MHz, Methanol · -d ₄ ) δ 8, 17 (dt, 1H 7.59 - 7 , 50 (m, 1H), 7 ', 32 (dd, 1H), 6.9 5 (d, 1H), 6.90 (d, 1H), 5, 03 - 4, 91 (m, 2H), 4.56 - 4.34 (m, 2H), 4.30 - 3, 88 (m, 4H), 3.76 - 3.55 ( m, 1H), 3.28 - 3, 10 (m, 1H), 3.10 - 2.9 6 (m, 4H), 2, 81 - 2, 62 (m, 2H), 2.53 (q.2.47 - 2, 33 (m, 1H), 2.31 - 2.14 ( m, 1H), 1.79 - 1, C) í2H), 1.07 (t, 3H), 0, 97 (td, 3H).
Example 3: (((S) -3- (dimethylamino) pyrrolidin ~ l ~ yl) ((S) -5ethyl-2- (6- (2 "ethyl" 5 "fluoro" 4 "hydroxyphenyl) -IH-indazol-3 --il) -
4,5,6,7-tetrahydro-3H-imidazo [4,5-c] pyridin-6-yl) methanone
fluoro-4-hydroxyphenyl) -1H-indazol-3-yl) -5-isopropyl-4,5,6,7tetrahydro-3A-imidazo [4,5-c] pyridine-6-carboxylic, TEA (179 mg, 0.310 mmol), (S) -A ', M-dimethylpyrrolidin-3-amine
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 96/165 (0.079 ml, 0.620 mmol) and DIPEA (0.162 ml, 0.930 mmol) in DMF (4 ml) was added HATU (177 mg, 0.46b mmol) and the reaction mixture was stirred at RT overnight. Hydrazine (5 eq) was added, the reaction mixture was concentrated and purified by preparative HPLC to provide the compound title TFA salt (107 mg, 44% yield).
(m / z): [M + H] ⁺ calculated for 560.31 of C31H38FN7O2 560.2 was found. ¹ H NMR (400 MHz, Methanol-dj) δ 8.21 (d,
1H), '' / 50 (s, 1H), 7, 2 6 (d, 1H), 6, 94 (d, 1 n), 6.90 (d, 1 h), 4.83 __ 4.6 6 (m, 1H), 4, 48 - 4.25 (m, 2H), 4.23 - 4.12 (m, 1H), 4, 12 - 3.93 (m, 2H ), 3, 93 - 3, 63 (m, 3H), 3.62 - 3.48 (m, 1H) , 3,2 6 - 3, 0 9 (m ., 1H), 2.98 (d, 6H) , 2, 67 - 2.57 (m, 1H), 2 _f 53 (q, 2H), 2, 44 - 2.12 (m, 1H), 1, 41 (t, 3H), 1.31 (d, 3H) , 1.05 (t, 3H) O
Example 5: (S) - (2 ~ (6- (2 "ethyl" 5 "fluoro" 4 "hydroxyphenyl) -ÍHindazol-3-yl) ~ 5 ~ isopropyl" 4,5,6,7 "tetrahydro ~ 3Jf ~ imidazo [4,5c] pyridin ~ 6 ”il), 4” diazepan ~ l ”il) methanone
F
[001611 To a solution of (S) -2- (6- (2 ~ ethyl ~ 5 ~ fluoro-4-hydroxyphenyl) -IH-indazol-3-yl) -5-isopropyl-4,5,6,7tetrahydro -SFI-imidazo [4,5-c] pyridine-6-carboxylic, TEA (30 mg, 0.052 mmol), 1-methylpiperazine (0.019 ml,
0.156 mmol) and DIPEA (0.036 ml, 0.208 mmol) in DMF (1 ml) was added HATU (29.6 mg, 0.078 mmol) and the reaction mixture was stirred at RT for 3 hours. Hydrazine (5 eq) was added, the reaction mixture was stirred at RT for
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 97/165 O ^3/7 1 ό 6 minutes in , concentrated and purified by preparative HPLC to give the TFA salt of the title compound (26.9 mg, 66% yield). (zn / z): [M + H] ⁺ calculated for 560.31 CjiHaaFN-10 560.2 was found.
Example 6: ((S) ~ 2 ~ (6 ~ (2 ~ ethyl ~ 5- ~ fluoro ~ 4 - hydroxyphenyl) ~ 1H ~ indazol ~ 3 ~ yl) ~ 5 ~ methyl ~ 4,5,6,7 ~ tetrahydro ~ 3H ~ imidazo [4,5 ~ cJpiridin-6-yl) ((R) -4- (2-hydroxyethyl) “2-methyl-piperazin-liljmethanone
fluoro “4 ~ hydroziphenyl) -IH-indazol-S-yl) -5 ~ methyl-4,5,6,7tetrahydro-3A-imidazo [4,5-c] pyridine-6-carboxylic, TFA (30 mg, 0.052 mmol), (x) ~ 2 ~ (S-methylpiperazin-1-yl) ethanol, 2 HCl (35.6 mg, 0.164 mmol), and DIPEA (0.057 ml, 0.328 mmol) in. DMF (1 ml) HATU (31.1 mg, 0.082 mmol) was added and the reaction mixture was stirred at RT overnight.
Hydrazine (8.57 μΐ, 0.273 mmol) was added, the reaction mixture was concentrated and purified by preparative HPLC to provide the compound title TEA salt (15.6 mg, 36% yield). (zn / z): [M + H] ⁺ calculated for 562.29 of C30H36FN7O3 was found 562.2.
Tariff of Attorney No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 98/165
Example 7: (£>) - (2- (6- (2 ~ ethyl ~ 5-fluoro-4 ~ hydroxyphenyl) 1H-indazol-3-yl) ~ 5 ~ propyl-4,5,6,7 “ tetrahydro-3H ~ imidazo [4,5cJpiridin-6-yl) (4- (2-hydroxyethyl) piperazin-1-yl) methanone
fluoro-4-hydroxyphenyl) -1H-indazol-3-yl) -5-propyl-4,5,6,7tetrahydro ~ 3.H-imidazo [4,5-c] pyridine-6-carboxylic, TFA (30 mg , 0.052 mmol), 2- (piperazin-1-yl) ethanol, 2HC1 (0.19 ml, 0.156 mmol) and DIPEA (0.027 ml, 0.156 mmol) in DMF (1.5 ml) was added HATU (29.6 mg, 0.078 mmol) and the reaction mixture was stirred at RT overnight.
Hydrazine (5 eq) was added, the reaction mixture was concentrated and purified by preparative HPLC to provide the TEA salt of the compound title (15.4 mg, 37% yield). (m / z): [M + H] ’calculated to 576.30
C31H38FN7O3 was found 576.2.
Preparation 10: tert-Butyl 2 ~ (6- (2 ~ ethyl ~ 5 ~ fluoro ~ 4 ~ methoxyphenyl) -1- (tetrahydro-2Jf-pyran ~ 2-yl) -1H-indazol-3-yl) -6 (methoxy (methyl) carbamoyl) -3 - ((2- (trimethylsilyl) ethoxy) methyl) 3,4,6,7-tetrahydro-5H-imidazo [4,5-c] pyridine-5-carboxylate (17 ')
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 99/165 [00164] To a stirred solution of 5- (tert butoxycarbonyl-2- (6- (2-ethyl-5-fluoro-4-methoxyphenyl) -1 acid ('cetra, idro - 2H - pyran-2 - i1) - iH — mdazol - 3 - yl) - 3 - ((2 - (trimethylsilyl) ethoxy) methyl) -4,5, 6,7-tetrahydro-37i-imidazo [4,5c] pyridine-6-carboxylic ( 16'j (4.0 g, 5.34 mmol) in DMF (20 ml) HATU (3.04 g, 8.01 mmol) was added in. The reaction mixture was stirred at RT for 30 minutes and were N, 0-dim.ethylhydroxylamine HCl (628 mg, 6.4 mmol) and
D1PEA (2.87 ml, 16.02 ml) and the reaction mixture was stirred at
TA for 2 H. 0 precipitate resu itant was tilted to provide the s crude oil that was pu rif icad ' 0 by chromatografic column (1 00-200) silica ~ gel, hey ui of co: m 2 0 - 3 0% E t. OAc: hexane for provide the title of the dog omitted (3.0 g, 71% Yield) like a white solid. (m / z): [M + H] ⁺ calculated to 793.40 from C41H57FN6O7SÍ was found ont r act 793.6.
Preparation 11: tert-Butyl 2- (6- (2-ethyl “5-fluoro-4“ methoxyphenyl) -1- (tetrahydro-2H-pyran-2-yl) -1H-indazol-3-yl) ~ 6 ~ formyl-3- ((2- (trimethylsilyl) ethoxy) methyl) -3,4,6,7-tetrahydro5H-imidazo [4,5-c] pyridine-5-carboxylate (12 ')
[001651 To a stirred solution of tert-butyl 2- (6- (2ethyl-5-fluoro ~ 4-methoxyphen.il) -1- (tetrahydro ~ 2.H-pyran-2-yl) -1.Hindazole-3 -yl) -6- (methoxy (methyl) carbamoyl) -3 - ((2 (trimethylsilyl) ethoxy) methyl) -3,4,6,7-tetrahydro ~ 5Aimidazo [4,5-c] pyridine-5-carbylate (17 ') (Preparation 10)
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 100/165 (3.0 g, 3.78 mmol) in dry THF (30 ml) 1 M of lithium aluminum hydride in THF (11.34 ml, 11.34 mmol) was added at 78 ° C under nitrogen and the reaction mixture was stirred for 1 h. Ethyl acetate was added dropwise to stop the reaction and the mixture was stirred at 0 ° C. To the resulting suspension, KHSO4 (30 ml) was added and the reaction mixture was extracted with EtOAc. The organic layer was washed with. brine, dried over anhydrous Na2SÜ4, filtered and concentrated at 40 ° C to provide the product title (2.4 g, 87% yield). The H ^± NMR conforms to the structure, (m / z): [M + H] ⁺ calculated for 734.37 of C39H52FN5O6SÍ 734.59 was found.
Preparation 12: 5-ethyl-2-fluoro-4- (3- (6 ”(pyrrolidin-lilmethyl) -4,5,6,7 ~ tetrahydro ~ 3H ~ imidazo [4,5 ~ c] pyridin-2 ~ yl ) 1H-indazol-6-yl) phenol
(a) tert-Butyl 2- (6- (2-ethyl-5-fluoro-4-methoxyphenyl) -1- (tetrahydro-217-pyran-2-yl) -lfí ^{r-indazol-3-yl)} -6- (pyrrolidin-1ylmethyl) -3 - ((2- (trimethylsilyl) ethoxy) -methyl) -3,4,6,7tetrahydro ”5.H“ imidazo [4,5-c] pyridine-5-carboxylate [00166] tert -Butyl 2- (6- (2-ethyl ~ 5-fluoro-4methoxyphenyl) -1- (tetrahydro-2H-pyran-2-yl) -1H-indazol-3-yl) -6formyl-3 - ((2- (trimethylsilyl) ethoxy) -methyl) -3,4,6,7-tetrahydro577-imidazo [4,5-c] pyridine-5-carboxylate (12 ') (50 mg, 0.068 mmol), pyrrolidine (0.028 ml, 0.341 mmol), acetic acid (0.039 ml, 0.681 mmol), and triacetoxyborohydride
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 101/165
87/136 sodium (144 mg, 0.681 mmol) was combined sequentially in DMF (1 ml) and stirred at RT overnight. The amorphous solid was removed in. EtOAc (10 ml) and washed with saturated aqueous NaHCOa solution (2 x 3 ml). The organic layers were dried in mgSCq and concentrated in vacuo to obtain a colorless oil (50 mg, 93% yield).
(b) 5-ethyl.-2-fluoro-4- (3- (6- (pyrrolidin-1-ylmethyl) -
4,5,6,7-tetrahydro-3.tt-imidazo [4,5-c] pyridin-2-yl) -IH-indazol6-yl) phenol [00167] The product from the previous step (0.05 g,
0.063 mmol) was dissolved in DCM (0.634 ml) and cooled to 0 ° C. Boron tribromide, 1 M in. DCM (0.634 ml, 0.634 mmol) drop by drop over several minutes and the reaction mixture slowly warmed to RT, stirred for 1 h, diluted with MeOH (10 ml) and concentrated in vacuo overnight. The crude residue was dissolved in dioxane (1 ml). Water (0.2 ml) was added followed by HC.) 4M in dioxane (1 ml) and the reaction mixture was stirred at RT for 30 minutes, frozen at - 78 ° C and lyophilized. The lyophilized powder was dissolved in water: 4: 1 acetic acid (10 ml), filtered by syringe and purified by preparative HPLC. The pure fractions were combined and lyophilized to provide the compound title TEA salt (32 mg, 73% yield). (m / z): [M + H] ⁺ calculated for
61.24 of C26H29FN6O2 was found 4 61.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 102/165 2nd Hey / 1B 6
Example 9: 5 ~ ethyl ~ 2 ~ fluoro ~ 4 ~ (3- (5 ~ methyl ~ 6 ~ (pyrrolidin-
1-ylmethyl) -4,5,6,7 "tetrahydro-3Jf-imidazo [4,5-c] pyridin-2-yl) - lfi-indazol-6-yl) phenol
fluoro-4- (3- (6- (pyrrolidin-1-ylmethyl) -4,5,6,7-tetrahydro-lifimidazo [4,5-c] pyridin-2-yl) -ln-indazol-6-yl ) phenol, 2TFA.
(Preparation 12) (32 mg, 0.046 mmol) were combined in MeOH (1 ml) at RT and stirred for 5 minutes. A solution of sodium cyanoborohydride (15 mg, 0239 mmol) in MeOH (1 ml) was added and the reaction mixture was stirred overnight. Sodium borohydride (40 mg) was added and the reaction mixture was stirred at RT for 3 h, concentrated, dissolved in water: 4: 1 acetic acid and purified by preparative HPLC to obtain the TFA salt of the compound title (10 mg, 30% yield). (m / z): [M + Hj ⁴ calculated for 475.25 C27H31FN6O 475.2 was found.
Preparation 13: (R) -pyrrolidin-3-yl 2 ~ (6 "(2 ~ ethyl-5 ~ fluoro-4-hydroxyphenyl) -1-indazol-3-yl) -4,5,6,7-tetrahydro3H- imidazo [4,5-c] pyridine-6-carboxylate
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 103/165
89/136 (a) 6 - ((R) -1- (tert-Butoxycarbonyl) pyrrolidin-3-yl) 5 (tert-butyl) 2- (6- (2-ethyl-5-fluoro-4-methoxyphenyl) -1 (tetrahydro-2H-pyran-2-yl) -lif-indazol-3-yl) -3- ((2 (trimethylsilyl) ethoxy) -methyl) -3,4,6,7-t.etrahydro-5 .Himidazo [4,5-c] pyridine-5,6-dicarboxylate [00169] To a solution of b- (tertbutoxycarbonyl) -2- (6- (2-ethyl-5-fluoro-4-methoxyphenyl) -1 acid (tetrahydro-2H-pyran-2-yl) -1H-inda.zol-3-yl) -3- ((2 (trimethylsilyl) ethoxy) methyl) -4,5,6,7-tetrahydro-3Himidazo [4, 5-c] pyridine-6-carboxylic (16 ') (50 mg, 0.067 mmol), tert-butyl (R) -3-hi.droxypyrroli.dine-lcarboxylate (312 mg, 1.667 mmol) and HATU (0.028 g, 0.073 mmol) in DMF (1.5 ml) DIPEA (0.046 m 0.267 mmol) was added and the reaction mixture was stirred at RT for 2.5 days and concentrated to provide the intermediate title which was used directly in the next step.
(b) (R) -pyrrolidin-3-yl 2- (6- (2-ethyl-5-fluoro-4hydroxyphenyl) -1H-indazol-3-yl) -4,5,6,7-tetrahydro-3Himidazo [ 4,5-c] pyridine-6-carboxylate [00170] The product from the previous step (61 mg,
0.066 mmol) was dissolved in DCM (1 ml) and cooled to 0 1 M sodium tribromide in HCl (1.5 ml, 1500 mmol) was added and the reaction mixture was stirred at RT for 1 h. Methanol (5 ml) was added and the concentrated reaction mixture, dissolved in 20% water / dioxane (2 ml) and 4 HCl in dioxane (2 ml, 8.00 mmol) was added. The reaction mixture was combined with the product of a run on the same scale and purified by preparative HPLC. The pure fractions were combined, frozen and lyophilized to provide the compound title TEA salt (20 mg, 21
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 104/165 / 13 6 yield) (m / z): [M + H] ⁺ calculated for 491.21 of C26H7FN6O3 was found 491.0.
Example 10: (R) -1-methylpyrrolidin-S-yl 2- (6- (2 ~ ethyl ~ 5 ~ fluoro ~ 4-hydroxyphenyl) -1H ~ indazol ~ 3 ~ yl) ~ 5 ~ methyl-4, 5,6,7tetrahydro-3J7-imidazo [4,5-c] pyridine ~ 6 ~ carboxylate
hydroxyphenyl) -lif-indazol-3-yl) -4,5,6,7-tetrahydro-3Aimidazo [4,5-c] pyridine-6-carboxylate, 2 TEA (12.1 mg, 0.017 mmol) and formaldehyde ( 1.504 pl, 0.020 mmol) were combined in MeOH (1 ml) and stirred for 5 min. Sodium cyanoborohydride (5.40 mg, 0.086 mmol) was added and the reaction mixture was stirred at RT overnight, concentrated and purified by preparative HPLC. The relevant fractions were combined, frozen and lyophilized. The product was dissolved in MeOH (1 ml). Sodium borohydride (50 mg, 1.322 mmol) was added and the reaction mixture was stirred at RT overnight, concentrated, dissolved in water: 1: 1 acetic acid (2 ml), filtered through a 0 syringe filter, 2 pm and purified by preparative HPL to obtain the compound title TEA salt (2.8 mg 22% yield). (m / z): [M + H] calculated for 519.24 of C28H3iFN ₆ O ₃ was found 519.1.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 105/165
91/136
Preparation 14: te.rt-Butü 2- (6- (2 ~ ethyl ~ 5 ~ fluoro ~ 4 ~ methoxyphenyl) -1- (tetrahydro-2H-pyran - 2-yl) -lJf-indazol-3-yl) ~ 6 ~ (hydroxymethyl) -3 - ((2- (trimethylsilyl) ethoxy) methyl) -3,4,6,7tetrahydro-5H-imidazo [4,5-c] pyridine-5-carboxylate
[001721 To a stirred solution of tert-butyl 2- (6- (2ethyl-5-fluoro-4-methoxyphenyl) -1- (tetrahydro-'2A-pyran-2-yl) -1Hindazol-3-yl) -6 -formyl-3 - ((2- (trimethylsilyl) ethoxymethyl) -
3,4,6,7-tetrahydro-5H-imidazo [4,5-c] pyridine-5-carboxylate (12 ') (1.8 g, 2.45 mmol) in MeOH (20 ml) NaBH ₄ was added (186 mg, 4.91 mmol) at 0 C in ^J. portions. The resulting reaction mixture was stirred at RT for 1 h, concentrated, diluted in ice water and extracted with DCM. The organic layers were combined, washed with brine, dried over anhydrous Na2SO ₄ and concentrated to provide the product title (1.5 g, 90% yield). The H ¹ NMR conforms to the structure, (m / z): [M + H] ⁺ calculated for 736.88 out of 0.398.54FNsOgSi 1'is found / 36.59.
Preparation 15: 4- (3- (5- (azetidin-3-yl) -6 (hydroxymethyl) -4,5,6,7-tetrahydro-3H-imidazo [4,5-c] pyridin-2yl) -lH -indazol-6-yl) -5-ethyl ”2 ~ fluorophenol
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 106/165 / 13 6 (a) 5-ethyl-2-fluoro-4- (3- (6- (hydroxymethyl) - 4,5,6,7tetrahydro-3H-imidazo [4,5-c] pyridin-2 -yl) -1H-indazol-6-yl) phenol [00173] tert-Butyl 2- (6- (2-ethyl-5-fluoro-4methoxyphenyl) -1- (tetrahydro-2H-pyran-2-yl) - 1H-indazol-3-yl) -6 (hydroxymethyl) -3 - ((2- (trimethylsilyl) ethoxy) methyl) -3,4,6,7tetrahydro-5H-imidazo [4,5-c] pyridine-5- carboxylate (0.5 g, 0.679 mmol) was dissolved in DCM (7.5 ml) and stirred at RT. Boron tribromide, IM in DCM (5.10 ml, 5.10 mmol) was added and after 10 minutes, additional 1 M sodium tribromide in DCM (5.10 ml, 5.10 mmol). The reaction mixture was stirred at RT for 2 n, diluted with MeOH (45 ml), stirred for 5 minutes and concentrated to dryness. The solid was dissolved in dioxane (5 ml) and water (1 ml), 4 M HCl in dioxane (5 ml, 20 mmol) was added and the reaction mixture was stirred at RT overnight, frozen and lyophilized. The residue was combined with the residue from a run on the 0.1 g scale, dissolved in 4: 1 water: acetic acid and purified by preparative HPLC. The pure fractions were combined and lyophilized to provide the title TEA salt (0.15 g, 42% yield) as a white powder, (m / z): [M + H] ^T calculated for 408, 18 for C22H22FN5O2 408 was found.
(b) 4- (3- (5- (azetidin-3-yl) -6- (hydroxymethyl) -4,5,6,7tetrahydro-37T-imidazo [4,5-c] pyridin-2-yl) - 1A-indazol-6-yl) -5 ethyl-2-furo or haeno1 [00174] To a solution of the product from the previous step (50 mg, 0.123 mmol) in MeOH (1227 μΐ) was added tertbutyl 3-oxoazetidine-l- carboxylate (210 mg, 1.227 mmol). The reaction mixture was stirred for 1 h and then cyanoborohydride (38.6 mg, 0.614 mmol) was added. The reaction mixture was stirred overnight, concentrated, treated with
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 107/165 η 3 /1.5 6
DCM (1 ml) and 4 M HCl in dioxane (1 ml), stirred for h, concentrated, coevaporated with --5 ml of EtOAc (5 mJ.), Dissolved in acetic acid: water 1: 1 and purified by HPLC preparative to provide the TFA salt of the intermediate title (19 mg, 33% yield) as a white powder, (m / z): [M + H] ⁺ calculated for 4 63.22 C25H27FN6O2 was found 4 63.
Example 11: 5-ethyl - 2- ~ fluoro ~ 4 “(3- (6- (hydroxymethyl) -5 (1-methylazetidin-3-yl) -4,5,6,7-tetrahydro-3H“ imidazo [ 4.5c] pyridin-2-yl) -1H-indazol-6-yl) phenol
[001751 Formaldehyde (3.67 pl, 0.049 mmol) and 4- (3- (5 (azetidin-3-yl) -6- (hydroxymethyl.) -4,5,6,7-tetrahydro ~ 3Himidazo [4,5 -c] pyridin-2-yl) -17f-indazol-6-yl) -5-ethyl-2-fluorophenol (19 mg, 0.041 mmol) were combined with MeOH (822 μΐ) at RT and stirred for 5 minutes. A solution of sodium cyanoborohydride (12.91 mg, 0.205 mmol) in MeOH (1 ml) and the reaction mixture was stirred overnight. Sodium borohydride (7.77 mg, 0.205 mmol) was added and the reaction mixture was stirred for 2 h, concentrated, dissolved in water: 4: 1 acetic acid, filtered through a syringe and purified by preparative HPLC to obtain the TFA salt of compound title (9.2 mg, 46% yield). (m / z): [M + H] ⁺ calculated for 4 7 / ', 2 3 of C26H29FN6O2 was found 4 / 7.2.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 108/165
94/136
Example 12: Methyl 2- (6- (2 ~ ethyl ~ 5 ~ fluoro ~ 4 ~ hydroxyphenyl) -1H-indazol-3-yl) -5- (1-methylpiperidin-4-yl) -

(a) Methyl 2- (6- (2-ethyl-5-fluoro-4-methoxyphenyl) -1 (tetrahydro-2 ü - pyran-2-yl) -17-indazol-3-yl) -3- ( (2 (trimethylsilyl) ethoxy) methyl) -4,5,6,7-tetrahydro-3H-imidazo [4,5-c] pyridine-6-carboxylate (30) [00176] A solution of methyl 5-benzyl-2 fluoro-4-methoxyphenyl) -1- (tetrahydro-2H-pyran-2-yl) -1Hindazol-3-1) -3 - ((2- (trimethylsilyl) ethoxy) methyl) -4,5,6,7tetrahydro- 3-imidazo [4,5-c] pyridine-6-carboxylate (5 ') (138 mg, 0.183 mmol), ammonium formate (346 mg, 5.49 mmol) and carbon palladium hydroxide (51.4 mg , 0.073 mmol) in. EtOH (3.66 ml) was purged with nitrogen for 10 min. The reaction flask was sealed and the mixture stirred at 80 ° C for 5 h, diluted with ethanol (10 ml), filtered with
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 109/165
95/136 syringe, concentrated and purified by preparative HPLC. The relevant fractions were combined, frozen and lyophilized to provide the intermediate title TFA salt (27 mg, 19% yield). (m / z): [M + H] 'calculated for 664.33 C35H ₄₅ FN ₅ O ₅ Si 665 was found.
(b) Methyl 2- (6- (2-ethyl-5-fluoro-4-methoxyphenyl) -1- (tetrahydro-2if-pyran-2-yl) -1H-indazol-3-yl) -5- (1methylpiperidin -4-yl) - 3 - ((2- (trimethylsilyl) ethoxy) methyl) -
4,5,6,7-tetrahydro-3H-imidazo [4,5-c] pyridine-6-carboxylate (31) [00177] The product from the previous step (30) (27 mg,
0.035 mmol), 1-methyl-4-piperidone (0.171 ml, 1.388 mmol) and acetic acid (0.079 ml, 1.388 mmol) were combined in. DMF sequence (2 ml). To the solution, sodium triacetoxyborohydride (294 mg, 1.388 mmol) was added and the reaction mixture was stirred at RT overnight, concentrated and purified by preparative HPLC. The relevant fractions were combined, concentrated to a clear oil to provide the intermediate title TFA salt (33.3 mg, 97% yield). (m./z): [M + H] calculated for 761.41 out of C41H57FN6O5SI 762 was found.
(c) Methyl 2- (6- (2 ~ ethyl ~ 5-fluoro “4 ~ hydroxyphenyl) -1H ~ indazol-3-yl) -5- (1-methylpiperidin-4-yl) -4,5,6, 7-tetrahydro3H-imidazo [4,5-c] pyridine-6-carboxylate [00178] The product from the previous step (31) (26.6 mg,
0.035 mmol) was dissolved in DCM (0.70 ml) and cooled to ° C and a 1 M solution of boron tribromide in DCM (0.350 ml, 0.350 mmol) was added. The reaction mixture was stirred at RT for 50 minutes, interrupted with MeOH (5 ml), concentrated and dissolved in 20% water / dioxane (1 ml). THE. solution was added 4.0 M HCl in. dioxane
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 110/165 (1 ml, 4.00 mmol) and the reaction mixture was stirred at ΤΑ overnight, concentrated and dissolved in MeOH (2 ml). To the solution, ethylenediamine (9.38 pg, 0.140 mmol) was added and the reaction mixture was stirred at RT for 7 h and purified by preparative HPLC. The relevant fractions were combined, frozen and lyophilized to provide the compound title TEA salt (9.2 mg, 35% yield). (m / z): [M + H] ⁺ calculated for 533.2 6 of C29H33FN6O3 was found 533.
Preparation 16: (6S) -tert-butyl 6 - ((S) -4- (tertbutoxycarbonyl) ~ 2 ~ methylpiperazine - 1 ~ carbonyl) -2- (6- (2 - et.il5 ~ fluoro-4- hydroxyphenyl) -1- (tet.hydro ~ 2H-pyran ~ 2 "yl) -1Hindazol-3-yl) -3 - ((2- (trimethylsilyl) ethoxy) methyl) -6,7-dihydro3H-imidazo [4, 5-c] pyridine-5 (4H) -carboxylate

[00179] (6S) -5- (tert-butoxycarbonyl) -2- (6- (2ethyl-5-fluoro-4-hydroxyphenyl) -1- (tetrahydro-2H-pyran-2-yl) lH-indazole- 3-yl) -3 - ((2- (trimethylsilyl) ethoxy) methyl) -4,5,6,7tetrahydro-3H-imidazo [4,5-c] pyridine-6-carboxylic (50 mg, 0.068 mmol), (S) -4-n-boc ~ 2-methylpiperazine (40.8 mg,
0.204 mmol) and DIPEA (0.036 ml, 0.204 mmol) were dissolved in DMF (1.0 ml), then HATU (38.8 mg,
0.102 mmol) and the reaction mixture was stirred at RT for hours. The reaction mixture was concentrated and the crude product was purified by chromatography on silica gel (0-100% EtOAc / Hexanes gradient) to obtain the compound title
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 111/165
97/136 (53 mg, 84% yield). (m / z): [M + H] + calculated for de CísHssFNvOsSí 919.1 was found
Preparation 17: ((S) -2- (6- (2-ethyl-5-fluoro-4 hydroxyphenyl) -HH-indazol-3-yl) -4,5,6,7 ~ -tetrahydro ~ 3H ~ imidazo [4,5-c] pyridin-6-yl) ((S) -2-methylpiperazin-1-yl) methanone

[00180] (6S) -tert-butyl 6- ((S) -4- (tert butoxycarbonyl) -2-methylpiperazine-1-carbonyl) -2- (6- (2-ethyl-
5-fluoro-4-hydroxyphenyl) -1- (tetrahydro-2H-pyran-2-yl) -1Hindazol-3-yl) -3 - ((2- (trimethylsilyl) ethoxy) methyl) -6,7-dihydro
3H-imidazo [4,5-c] pyridine-5 (4H) -carboxylate (52.7 mg,
0.057 mmol) was dissolved in dioxane (1.0 ml) and water (0.2 ml), then 4M HCl in dioxane (1.0 ml, 4.00 mmol) was added and the reaction mixture was stirred at 50 ° C for 16 hours. The reaction mixture was frozen and lyophilized and the resulting solid was dissolved in 2 ml of MeOH. Ethylenediamine (0.015 ml, 0.230 mmol) and sodium borohydride (13.03 mg, 0.344 mmol) were added and the reaction mixture was stirred at room temperature for 12 hours. The solution was concentrated and purified by preparative HPLC to provide the compound title TEA salt (18 mg, 42% yield). (m / z): [M + H] + calculated for 504.6 C27H30FN7O2 504.5 was found.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 112/165 μ θ 11 σ 6
Examples 2-15: (S) -2,4-dimethylpiperaz; in-1-yl) ((S) -2- (6 ~ (2 "ethyl" 5 "fluoro" 4 "hydroxyphenyl) -1H-indazole-3 -il) -S-methyl-
4,5,6,7-tetrahydro-3H-imidazo [4,5-c] pyridin-6-yl) methanone

[001811
2- (6- (2-ethyl-5-fluoro-4 ~ hydroxyphenyl) -1H indazol-3-yl) -4,5,6,7-tetrahydro-3H-imidazo [4,5-cJpiridin-6il) ( (S) -2-methylpiperazin-1-yl) methanone, 2TFA (17.7 mg,
0.024 mmol) and formaldehyde, 37% by weight. water (4.50 pl,
0.060 mmol) were dissolved in methanol (1.0 ml) and then sodium cyanoborohydride (7.60 mg, 0.121 mmol) was added and the reaction mixture was stirred at room temperature for 3 hours, sodium borohydride (0.024 mmol) to remove any remaining formaldehyde, then the solution was concentrated. The crude product was purified by preparative HPLC to provide the TFA salt of the compound title (12 mg, 66% yield). (m / z): [M + H] + calculated for 532.6 of C29H34FN7O2 was found 532.2.
Examples 12-14: (R) -N- (2- (diethylamino) ethyl) -5-ethyl-2- (6 (2-ethyl “5“ fluorO “4-hydroxyphenyl) -1H-indazol-3-yl) -N-methyl-
4,5,6,7-tetrahydro-3H ”imidazo [4,5-c] pyridine-6-carboxamido

Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 113/165
99/136 [00182] (A) -5-Ethyl-2- (6- (2-ethyl-5-fluoro-4hydroxyphenyl) -1H-indazol-3-yl) -4,5,6,7-tetrahydro acid -3Himidazo [4,5-c] pyridine-6-carboxylic acid, TFA (40 mg, 0.071 mmol), NI, N1-diethyl-N2-methylethylene-1,2-diamine.
(0.046 ml, 0.284 mmol), and DTPEA (0.062 ml, 0.355 mmol) were dissolved in DMF (2.0 ml), then HATU (32.4 mg, 0.085 mmol) was added and the reaction mixture was stirred at temperature 16 hours. Hydrazine (0.011 ml, 0.355 mmol) was added and the reaction mixture was stirred at room temperature for 10 minutes. The solution was concentrated and purified by preparative HPLC to provide the TEA salt of the compound title (24 mg, 42% yield).
(m / z): [M + H] + calculated for 562.7 of C31H40FN7O2 was found 562.7.
[00183] Using similar synthetic methods, the compounds of Tables 1-19 were prepared. In the following tables, a blank in. any column indicates a hydrogen atom, a * in a structure in the header of a table indicates a chiral center, the notation (A) or (S) in front of a substitute denotes the configuration of the carbon atom to which the substitute has been attached .
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 114/165
Table 1
Ex.At the. * R ¹ R ³ Formula [M + H] ⁺ calculated [M + H] ⁺ found 1-1H ch ₃ C27H30FN7O2 5 0 4.24 504.1 1-2H IPr C 2 9 H 3 4 FN 7 O2 532.28 532.3 1-3H CH2CH2OH C28H32FN7O3 534.26 534.2 1-4H cpent.il C31H36FN7O2 5 5 8 2 9 558.3 1-5H chexi C32H38FN7O2 572.31 572.3 1-6H cpropyl C29H32FN7O2 530.26 53 0.2 1-7 ch ₃ C28H32FN7O2 518.2 6 518.1 1-8ch ₃ cpropyl C30H34FN7O2 544.28 544.2 1-9CH3 chexi C33H40FN7O2 586.32 5 8 6.2 1-10CH3 CH2CH2OH C 2 9 H 3 4 FN 7 0 3 548.27 54 8 1-11CH3 cpentil C32H38FN7O2 572.31 572 1-12ch ₃ iPr C30H36FN7O2 54 6, 29 54 7 1-13C2H5 iPr C31H38FN7O2 5 6 0.31 5 60 1-14C2H5 CH ₂ CH ₂ OH C 3 0 H 3 6 FN 7 0 3 562.29 5 62 1-15 (S) 3 3 iPr C 3 0 H 3 6 r N 7 U 2 54 6, 2 9 546 1-16 (R) ch ₃ iPr C30H36FN7O2 546, 29 546 1-171. r 1 CH2CH2OH C31H38FN7O3 57 6, 3 0 57 6.2 1-18iThT CH2CH2OH C 31H 3 8 FN 7 0 3 57 6, 30 576.9 1-19 (S) ch ₃ CH2CH2OH C29H34FN7O3 548.27 548.2 1-2 0 (R) ch ₃ CH2CH2OH C29H34FN7O3 548.27 548.2 1-21 (S) CH3 ch ₃ C28H32FN7O2 518.2 6 518.3
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 115/165
Ex.At the. * R ¹ R ³ Formula [M + H] ⁺ calculated [M + H] ⁺ found 1-22 (R) ch ₃ ch ₃ C ₂₈ H ₃ 2FN ₇ O ₂ 518.26 518.3 1-231 hT IPr C32H40FN7O2 574.32 57 3, 8 1-24CH3 tBu OsiHvgjj N7O2 560.31 559.7 1-25C ₂ H ₅ t.BU C32H40FR7O2 574.32 57 3.7 1-2 6nPr t.Bu C33H42FN7O2 588.34 587.7 1-27Ί P Ύ ' tBu C 3 3 H 4 21N 7 0 2 588.34 587, 8 1-2 8 (R) nPr UH2RH2 OH C31H30FN7O3. 57 6, 3 0 57 6.2 1-2 9 (S) c ₂ h ₅ CH2CH2OH C30H36FN7O3 562.29 5 62.5 1-3 0 (R) C2H5 CH2CH2OH C30H36FN7O3 562.29 5 62.5 1-31CH3 H C27H30FN7O2 5 0 4.24 504.2 1-32C2H5 ch ₃ c 2 9 h 3 4 m 7 o ₂ 532.28 532.2 1-33r, pr ch ₃ C30H36FN7O2 .546 / 29 546.2 1-34iPr ch ₃ C ₃ oH ₃₆ FN ₇ 02 54 6, 29 54 6, 3 1-35Ui P Ύ ~C29H34FN7O2 532.28 532.2 1-3 6 (S) C ₂ H ₅ tBu C232H4UFN7O2 574.32 57 4.3 1-37 (R) C ₂ H ₅ tBu C32H40FN7O2 574.32 57 4.3 1-381. r Γ H C ₂₉ H34FN7O ₂ 532.28 532.2 1-39 (R) 1 Rr H C2 9H3 4 m 7 O ₂ 532.28 532.2 1-40 (S) 1 Rr H C2 9H3 4 m 7 0 ₂ 532.28 532.2
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 116/165
Ex.At the. * R ^4a _R 4b r ^4c Formula [M + H] ⁺ calculated [M + H] ⁺ found 2-1 (Si ch ₃ (R) CH ₃ 6-3 oH 3 ΙΆ-Ί 54 6.29 b4 Ό 2-2(R) CH ₃C 2 9 Η 3 4 FN 7 Ο 2 532.28 532 O(S) ch ₃c _2S h ₃₄ fn ₇ o ₂ 532.28 532 2-4(S) ch ₃ (R) CH ₃ C ₃₀ H ₃ gFN ₇ O ₂ 5 4 6.2 9 54 6 2-5 (S) ÜH3C ₂ aH ₃₄ FN ₇ O ₂ 532.28 532 2-6 (R) CH ₃ C 2 9 H 3 4 FN 7 0 2 532.28 532 2-7(R) CH ₃ (R) CH ₃ c ₃₀ h ₃₆ fn ₇ o ₂ 546.29 546 2-8 ( _λ S) (R) CH ₃C ₂ gH ₃₄ FN7U2 532.28 5 3 2 2-9 (Λ) (R) CH ₃C29H34E ^ 7 ^ 2 532.28 532 2-10 (S)(R) CH ₃ C 2 9 H 3 4 FN 7 O 2 532.28 531.7 2-11 (S)(S) ch ₃ c _2S h ₃₄ fn ₇ o ₂ 532.28 531, 8 2-12 (R)(S ') ch ₃ C ₂ 9H _{3 4} FN- O ₂ 532.28 531.7 2-13 (R)(R) CH ₃ c _2S h ₃₄ fn ₇ o ₂ 532.28 531.7 2-14(R) CH ₃ (S ') ch ₃ c ₃₀ h ₃₆ fn ₇ o ₂ 546.29 54 6 2-15 (S) (S) ch ₃c ₂₉ h ₃₄ fn ₇ o ₂ 532.28 532.3 2-16 k 5) (S) CH ₃ (R) CH ₃ C ₃ oH ₃₆ FN ₇ 0 ₂ 54 6, 2 9 54 6, 3 2-17 (S) (R) CH ₃ (R) CH ₃ 6-3 oH 3 el 1 ^ 7 ^ 2 546.29 54 6, 6 2-18 (R) (S) ch ₃C 2 9 H 3 4 FN 7 O 2 532.28 532.2 2-19 (R) (S ') ch ₃ (R) CH ₃ c ₃₀ h ₃₆ fn ₇ o ₂ 54 6.29 546, 6 2-20 (R) (R) CH ₃ (R) CH ₃ C3oH ₃ gFN ₇ 0 ₂ 5 4 6.2 9 546, 2 2-21(S) c ₂ h ₅C13oH ₃ gFN ₇ 0 ₂ 54 6.2 9 545.7 2-22(λ) C ₂ H ₅C.3oH ₃ gFN ₇ 0 ₂ 546.29 545, 8
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 117/165
3/13 6
Ex.At the. * R ¹ R ^4a R ⁴¹³ _r 4c Formula [M + H] ⁺ calculated [M + H] ⁺ found 3-1H(S) CH ₃ (R) CH ₃ C28H ₃₂ FN ₇ O ₂ 518.26 518.2 3-2ch ₃ (R.) CH ₃C ₂₈ H ₃₂ FN ₇ O ₂ 518.26 517.7 3 3ch ₃ (R) CH ₃ (R) CH ₃ C ₂ 9H ₃₄ FN ₇ O ₂ 532.28 532.2 3 - willch ₃ (S) ch ₃ C ₂₈ H ₃₂ FN ₇ O ₂ 518.2 6 518.2 3-5ch ₃ (S) CH ₃ (-R) CH ₃ C ₂ 9H ₃ 45N ₇ O ₂ 532.28 532.2 3 “6ch ₃ (S) ch ₃C 2 8 H 3 2 £ 'N 7 0 2 518.26 518.2 3-7nPr (S) ch ₃C3oH ₃ sFN ₇ 0 ₂ 546.29 546 3 ™ 8nPr (.R) CH ₃c ₃₀ h ₃₆ fn ₇ o ₂ 546.29 546 3-9nPr (S) ch ₃ (R) CH ₃ C ₃ 1H ₃₈ FN ₇ Q ₂ 560.31 560 3-10nPr (R.) CH ₃ (R) CH ₃ C ₃ iH ₃₈ FN ₇ O ₂ 560.31 560 3-11nPr(S) ch ₃ C ₃ oH ₃ gFN ₇ 0 ₂ 54 6.29 54 6 3-121P r (S) CH ₃ (R) CH ₃ C ₃ iH ₃₈ FN ₇ O ₂ 560.31 560.2 3-13iPr (S) CH ₃C ₃ oH ₃ 6FN ₇ 0 ₂ 5 4 6.2 9 546, 2 3-14iPr(S) ch ₃ C ₃ 0 H ₃ g FN ₇ 0 2 14 6.2 9 546, 3 3 - 15iPr (R) CH ₃C ₃ oH ₃₆ FN ₇ 0 ₂ 546.29 546.2 3 -16IPr (R) CH ₃ (R) CH ₃ C ₃ iH ₃₈ FN ₇ O ₂ 560.31 5 6 0, 3 3-17 (S) ch ₃ (-R) CH ₃C ₂ 8H ₃₂ FN ₇ Q ₂ 518.26 518.4 3-18 (R) ch ₃ (R) CH ₃c ₂₈ h ₃₂ fn ₇ 0 ₂ 518.26 518.3 3-19 1 ~ D ^ -! ch ₃ (S) C2H5 C ₂ 9H ₃₄ FN ₇ O ₂ 532.28 532.3 3-20 R} ch ₃ (R) C2H5 C ₂ 9H _3i nFN ₇ O ₂ 532.28 532.2 3-21 1 <Z \ '-s / nPr (R) CH ₃ (-R) CH ₃ C ₃ iH ₃₈ FN ₇ O ₂ 560.31 560, 3
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 118/165
4/136
Ex.At the. * R ¹ R ^4a _R 4b _r 4c Formula [M + H] ⁺ calculated [M + H] ⁺ found 3-22 (S) ch ₃ (R) C ₂ H ₅5 _2e H ₃₄ FN ₇ O ₂ 532.28 532.3 3-23 (S) nPr(S) CH ₃ ^ 28 ^ 32 FN7O2 518.26 517.7 3-24 (~ D ^ -! nPr (R) ch ₃ (R) CH ₃ C ₂ ç, H ₃₄ FN ₇ O ₂ 532.28 532.2 3-25 R} nPr(S) ch ₃ 5-28H ₃₂ FN ₇ O ₂ 518.2 6 518.2 í - 7 6 £ 1 ch ₃ 1, S) C ₂ H ₃U9H ₃₄ FN ₇ O ₂ 532.28 532.2
Table 4
F
Ex.At the. * & R ¹ R ³ Formula [M + H] ⁺ calculated [Μ + ΗΓfound 4 -1(R) ch ₃ ch ₃ C ₂₉ H ₃₄ FN ₇ O ₃ 548.27 54 8.7 4-2(. S) ch ₃ ch ₃ C ₂ qH ₃₄ F'N ₇ O ₃ 548.27 54 8.7 4-3 (R) (S) C ₂ H, 5 ch ₃ C ₃₀ H ₃₆ F'N ₇ O ₃ 562.29 5 62.2 4-4 (R) (R) c ₂ h ₅ ch ₃ C ₃ 0 H ₃ 6 FN ₇ 0 3 562.29 5 62.2 4-5 (R) (R) nPr ch ₃ C31H30FN7O3 57 6, 3 0 57 6, 3 4-6 (R) (S) nPr ch ₃ C ₃ 1H ₃ 8FN ₇ O3 5 Ί 6, 3 0 576.2 4-7 (R) (R) iPr ch ₃ C3iH ₃₈ FN ₇ O ₃ 57 6, 3 0 57 6.2 4-8 (R) (S') iPr ch ₃ C 31H 3 s FN ₇ 0 3 57 6, 30 576.2 4-9 (S) (R) iPr ch ₃ C3iH ₃₈ FN ₇ O ₃ 5 7 6, 3 0 576.2 4-10 (S) (R) c ₂ h ₅ ch ₃ C30H36FN7O3 562.29 5 62 4-11 (S) (. S) C ₂ H, 5 ch ₃ C3oH ₃₆ F'N ₇ 0 ₃ 562.29 562 4-12 (S) (R) nPr ch ₃ CsiHsgFN-Os 57 6, 3 0 57 6 4-13 (S') (S) iPr ch ₃ C / 31H30FN7O3 5 Ί 6, 3 0 576.2
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 119/165
Table 5
Ex.At the. * • »^ 43.1 r ~ j4â, 2 _R 4b2 R ³ Formula calculated [MfH] ⁺ found 5-1Create 3 Create 3 CH ₃ c ₃₀ h ₃₆ fn ₇ o ₂ 5 4 6.29 54 6 5-2 A) Τ '* b-í o Τ '* b-í o ch ₃ c ₃₀ h ₃₆ fn ₇ o ₂ 54 6.2 9 546, 2 5-3 (S) ch ₃ c ₃₀ h ₃₆ fn ₇ o ₂ 546, 29 54 6, 2 5-4 - (CH ₂ ) 3 ~ ch ₃ C ₃ iH ₃₆ FN ₇ O ₂ 558.29 557.8 5- 5(R) CH ₃ k, c C3oH ₃ > 5J N ₇ 0 ₂ 54 6, 29 54 6, 2 5-6ÍR} CH ₃ iPr C ₃ iH _3g FN ₇ O ₂ 560.31 560.2 5-7(R) CH ₃ cu C ₃₂ H ₃₈ FN ₇ O ₂ 572.31 571, 6 5-8(R) CH ₃ (The) c ₃₀ h ₃₆ fn ₇ o ₃ 563 _Λ 2 9 562 5-9 A) (R; CH ₃ (The) c ₃₀ h ₃₆ fn ₇ o ₃ 562 _z 2 9 562.2 5-10 A) ~ (ch ₂ i ₃ - H c ₃₀ h ₃₄ fn ₇ o ₂ 544.28 544.3 5-11 (S) - (CH ₂ ) ₃ - H c ₃₀ h ₃₄ fn ₇ o ₂ 544.28 544.2
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 120/165
Ex.At the.* R ¹ R ³ Formula [M + H] ⁺ calculated [M + H] ⁺ found Ό “.1. THEH H C ₂₇ H ₂₃ FN ₇ O ₂ 502.23 502.2 6 “2 THE ch ₃ c ₂₉ h ₃₂ fn ₇ o ₂ 530.26 530 6 “3 THEnPr H c ₃₀ h ₃₄ fn ₇ o ₂ 544.28 344 _Λ 3 6-4 THEnPr ch ₃ C ₃ iH ₃₆ FN ₇ O ₂ 558.29 558.3 Ό 2 THEiPr ch ₃ C ₃ iH ₃ 6 fn ₇ o ₂ 558.29 558.3 6-6 THEipr H C ₃₀ H ₃₄ FN ₇ O ₂ 544.28 544.2 6-7 THE (THE) nPr ch ₃ c ₃₁ h ₃₆ fn ₇ o ₂ 558.29 558.2 6 ”8 B (S') c ₂ h ₅ ch ₃ C30H34FN7O2 544.28 Γ - '. / 4 4 ο xJ ^z -i! .J 6-9 THE (S') 0'2 TO 5 ch ₃ c ₃₀ h ₃₄ fn ₇ o ₂ 544.28 544.2 6-10 B (S) nPr ch ₃ C ₃ iH ₃₅ FN ₇ O ₂ 558.29 5 8 6-11 P I ~ D ^ -! nPr ch ₃ C ₃₁ H ₃ 6FN ₇ O ₂ 558.29 558.2 6-12 THEc ₂ h ₃ ch ₃ c ₃₀ h ₃₄ fn ₇ o ₂ 544.28 544.3 6 -13 B (THE) c ₂ h ₅ ch ₃ c ₃₀ h ₃₄ fn ₇ o ₂ 544.28 544.3
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 121/165
Table 7
Ex.At the. * R ¹ R ³ Formula [M + H] ⁺ calculated [M + H] ⁺ found 7-1CH3 ch ₃ C 2 y H 3 4 FN 7 0 2 532.28 532.2 * 7 QCH ₃ iPr CeiHagFNvCA 560.31 5 6 0, 1 7 - 3CH3 cpropyl C 31H 3 q F N 7 u 2 558.29 558.1 7-4/ - ¹ -, cpentil C33H40FN7Q2 5 8 6, .3 2 586.0 7-5f '* _o cbut i1 C32H38FN7O2 572.31 572.1 7-6A '· yj _o (The) C31H38 FN7O3 57 6, 3 0 576.0 7 - 7iPr ch ₃ C31H ₃₈ FN7O ₂ 5 6 0.31 560.3 7-8iPr iPr C33H42FN7O2 588.34 588.2 7-9 (.R) IPr ch ₃ Cai-tlag FN7O2 560.31 560.2 7-10CH ₃ C28H32FN7O2 518.26 518.2 7-11nPr £ 4 C 3 0 H 3 6 F N 7 U 2 546.29 546.2 7-12iPrC30H36FN7O2 5 4 6, 2 9 546.2
(a) (CH ₂ ) ₂ OCH ₃
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 122/165
Table 9
Ex.At the. * ** R ¹ R ⁵ R ^s Formula [M + H] ⁺ C3.1 cu 1 sdo [MtH] ⁺ found 9-1(S) ch ₃  (UH2) 4 '“ C31 ΗβζΓ N7O2 558.29 558.2 9 ™ 2(S) ch ₃ - (CH ₂ ) ₅ - c ₃₂ h ₃₈ fn ₇ o ₂ 572.31 572.3 9-3(S) c ₂ h ₅ - (CH ₂ ) ₄ - c ₃₂ h ₃₃ fn ₇ o ₂ 572.31 572.2 O-Zi~ ±(β) ch ₃ - (CH ₂ ) ₄ - C ₃ iH ₃₅ FN ₇ O ₂ 558.29 558.2 9-5 ( S) (S) ch ₃ - (CH ₂ ) ₄ - C ₃ iH ₃ 6FN ₇ O ₂ 558.29 558.2 9- 6 go) (s) ch ₃ (CH ₂ ) ₄ - C ₃ iH ₃₆ FN ₇ O ₂ 558.29 558.2 9-7 (S) (S) ch ₃ - (CH ₂ ) 5- c ₃₂ h ₃₈ fn ₇ o ₂ 572.31 572.2 9-8 (R) (S) ch ₃ - (CH ₂ ) 5- c ₃₂ h ₃₈ fn ₇ o ₂ 572.31 b! 2 f 3 9-9(S) nPr  (UH2) 4 '“ C33H4QL ’N7O2 586.32 585, 8 9-10(S) .iPr - (ch ₂ ) ₄ - C ₃₃ H ₄₀ FN ₇ O ₂ 586.32 585, 8 9-11(β) iPr - (CH ₂ ) ₄ - C ₃₃ H ₄₀ FN ₇ O ₂ 586.32 58 6, 3 9-12(β) c ₂ h ₅ (CH ₂ ) ₄ - c ₃₂ h ₃₈ fn ₇ o ₂ 572.31 572.2 9-1.3(β) nPr - (CH ₂ ) ₄ - c ₃₃ h ₄₀ fn ₇ o ₂ 5 8 6 _f 3 2 586, 2 9-14( _λ S) nPr ch ₃ Ch 3 C ₃ iH ₃₈ FN ₇ O ₂ 560.31 560.4 9-15(β) ch ₃ - (CH ₂ ) 5- c ₃₂ h ₃₈ fn ₇ o ₂ 572.31 571.7 C) ___ 3 (A,(S) ch ₃ CH, Chi3 C29H34H N7O2 , 5 3 2,2 8 531.8 9-17 (S) (S) c ₂ h ₅ “(CH ₂ ) ₄ - c ₃₂ h ₃₈ fn ₇ o ₂ 572.31 572.3 9-18 GO) (S) c ₂ h ₅ - (CH ₂ ) ₄ - C ₃₂ H ₃₈ FN ₇ O ₂ 572.31 572.3 1 Q(β) iPr ch ₃ T-T C ₃ iH ₃₈ FN ₇ O ₂ 560.31 560.3 9-2 0 (S) (S') nPr ch ₃ H C3iH33jj N7O2 560.31 560.2 9-21 (β) (S) nPr ch ₃ C ₃ 1H ₃₈ FN ₇ O ₂ 560.31 5 6 8 3 9-22(S) nPr - (CH ₂ ) 5- C ₃₄ H ₄₂ FN ₇ O ₂ 600.34 600.3
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 123/165
Ex.At the. *R ¹ R ⁵ R ^s Formula [M + H] ⁺ calculated [M + H] ⁺ found 9-23(S) c ₂ h ₅ ch ₃ ch ₃ c ₃₀ h ₃₆ fn ₇ o ₂ 54 6.2 9 54 6.2 9-24(S) c ₂ h ₅ - (CH ₂ ) 5- c ₃₃ h ₄₀ fn ₇ o ₂ 586.32 58 6, 3 9 2 5 (S') (S') iPr (CH ₂ ) ₄ - C ₃₃ H4 ₀ FN ₇ O ₂ 586.32 5 8 6, 6 9-26 (R) (S') 1P r - (CH ₂ ) 4- c ₃₃ H4 ₀ fn ₇ o ₂ 5 8 6 _f 3 2 586, 3 9-27(S) iPr - (CH ₂ ) 5 - c ₃₄ H4 ₂ fn ₇ o ₂ 600.34 600.3 9-2 8 (R) (S) iPr ch ₃ f '' _ C 31H3 A t N 7 O2 560.31 560.2 9-2 9 (S') (S') iPr  (UH2) 5 ‘ C ₃ 4H4 ₂ FN ₇ O ₂ 6 0 0, 8> 3 9 600, 3 9-30 (R) (S') iPr ... (CH ₂ ) ₅ ... C ₃ 4H4 ₂ FN ₇ O ₂ 6 0 0, 8> 3 9 600, 3 9-31 (S) (S) nPr - (CH ₂ ) 5- C ₃ 4H ₄₂ FN ₇ O ₂ 6 0 0, 3 3 9 600.6 9-32 (R) (S) nPr - (CH ₂ ) 5- C ₃ 4H ₄₂ FN ₇ O ₂ 6 0 0, 3 3 9 6 0 0, 6 9-33 (-K) (S') C ₂ H, 5 ch ₃ f · ’T-T -, C ₃₀ H ₃₅ FN ₇ O2 546, 292 54 6.2 9-34 (S') (S') c ₂ h ₅ ch ₃ T-T -, C _{3 0} H ₃ 5 fn ₇ o ₂ 54 6, 2 92 5 4 6, 2
Ex.At the. * ** R ¹ R ⁵ R ⁶ Formula [M + H] ⁺ calculated [M + H] ⁺ found 10-1 (R) (S) ch ₃ ch ₃ H -, c ₃₀ h ₃₆ fn ₇ o ₂ 546, 29 546, 3 10-2 (R) (S) c ₂ h ₅ ch ₃ C ₃ iH ₃₈ FN ₇ O ₂ 5 6 0.31 560, 3 10-3 (R) (R) c ₂ h ₅ ch ₃ Ch 3 C ₃ iH ₃₈ FN ₇ O ₂ 560.31 the 6 0, 3 10-4 (S) (S') ch ₃ ch ₃ f ' ¹ y.7 C ₃ oH ₃ 6FN ₇ 0 ₂ 5 4 6.2 9 54 6.2 10-5 (S) (R) ch ₃ ch ₃ Chia C ₃ oH ₃₆ FN ₇ 0 ₂ 54 6.29 54 6.2 10-6 (S) (S) c ₂ h ₅ ch ₃ L.H 3 C ₃ iH ₃₈ FN ₇ O ₂ 560.31 560.3 10-7 (S) (R) c ₂ h ₅ ch ₃ ch ₃ C ₃ iH ₃₈ FN ₇ O ₂ 5 6 0.31 5 60.2
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 124/165
110/136
Table 11
F
Ex.At the. * ** R ¹ Formula [M + H] ⁺ calculated found 11-1(Sj CH3 C32H3A FN7O2 572.31 571.8 11-2(S) C2H5 Q33R4 0 - Π7 O2 586.32 58 6.8 11-3(,S) RpP C34H42FN7O2 6 0 0, 3 4 599.7 11-4Í.S) iPr C34H42FN7O2 6 0 0.3 4 599.8 11-5 í R '> (s) CH3 C32H38FN7O2 572.31 572.8
Table 12
F
HO
Ex.At the. * R ¹ R ¹⁰ R ¹¹ Formula [M + H] ⁺ calculated [M + H] ⁺ found 12-1ch ₃ C2H5 C2H5 C30H38FN7O2 548.31 54 7.8 12-2ch ₃ ch ₃ CH3 C 2 8 H 3 4 L · N 7 0 2 520.28 520.3 12-3ch ₃ - (CH ₂ ) 4- C 3 0 H 3 6 r N 7 U 2 54 6, 2 9 546.2 12-4iPr ch ₃ ch ₃ C / 30H30FN7O2 548.31 548.2 12 ~ 5IPr - (CH ₂ ) 4- C32H40FN7O2 574.32 57 4, 6 12-6nPr CH3 Chi 3 C 3 0 H 3 g HN 7 O2 548.31 548.3 12-7nPr "(CH2) 4 '"' C32H4 0 FN 7O2 574.32 574.3
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 125/165
111/136
12-8c ₂ h ₅ C ₂ .V5 c ₂ h ₅ C31H40FN7O2 5 2 2 _Λ 32 5 61, 8 12-9nPr C2H5 C2H5 C ₃₂ H ₄₂ FN7O ₂ 57 6, 34 57 5 9 12-10iPr C2H5 c ₂ H ₅ ^ 7θ2 57 6, 34 575.8 12-11 (S) iPr ch ₃ CHv C3oH ₃₈ FN70 ₂ 54 8, 31 548.4 12-12 ! Laugh IPr ch ₃ ch ₃ C3oH ₃₈ FN ₇ 02 54 8, 31 54 8.2 12-13 (S} c ₂ h ₅ c ₂ h ₅ c ₂ h ₅ C31H40FN7O2 5 62, 32 5 62.7 12-14 R'i C ₂ Ho C 0 H c. ç. ₂ h ₅ C31H40FN7O2 562, 32 5 62.7 12-15 S) iPr C 9 i'Í ^c · c ₂ h ₅ C32H42FN7O2 57 6, 34 57 6, 7 12-16 {R-'i iPr C ₂ .V5 c ₂ h ₅ C32H42FN7O2 5 Ί 6, 34 5 7 6, 6
Table 13
Ex.At the. * R ¹ R ¹² Formula [M + H] ⁺ calculated [M4-H] ⁺ found 13-1ch ₃ C '' R -j 330 / 7363'14702 54 6, 2 9 54 6 13-2ΓιΡΓ Ch O C32H40 Η N7O2 574.32 O / f 2. 13-3iPr CR .., C ₃₂ H ₄ oFN70 ₂ 574.32 574.3 13-4C ₂ H ₅ ch ₃ C31H38FN7O2 560.31 560.2 13-5 (S} 73 P Ύ ~ ch ₃ C32H40FN7O2 574.32 .5 / 4 _Λ 6 13-6 (THE) nPr ch ₃ R32H40- N7O2 574.32 574, 6 13-7P .P I C ₂ H ₅ C33H42FN7O2 588.34 58 8 13-8ΙΊ p '' iPr C34H.44FN7O2 602.35 602 13-9cu C '' R -j C 3 3 H 4 Q £ 'N 7 0 2 586.32 58 7 13-10cu ch ₂ ch ₂ oh C34H / 12 ^ ^ 7 ^ 3 616.33 616 13-11 (S) nPr C ₂ H ₅ C3 ₃ H42FN ₇ O ₂ 588.34 588.6
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 126/165
112/136
13-12 (.R) nPr c ₂ h ₅ C33H42FN7O2 588.34 588.6 13-13 (S) nPr iPr C34H44FN7O2 602.35 602.7 13-14 (R) nPr iPr U34H44L ¹ N7O2 602.35 6 0 2.8
Table 14
Ex.At the. *R ¹ R ¹² Formula [M + H] ⁺ calculated [M + H] ⁺ found 14-11 Y} f '* ch ₃ C30H36FN7O2 5 4 6, 29 54 6 14-21 rc / C '' I-J -j ch ₃ C30H36ÍJ N7O2 546, 29 54 7 14-3 1 S'j 1 S'j Ch q CH3 C30H36FN7O2 .546 / 29 545, 8 14-4 (S) (R'i CH3 G * Ί-Τ C30H36FN7O2 54 6, 29 54 5.7 14-5(R) IPr f '* _o C32H40FN7O2 574.32 575 14-6 (jR) (S) CH3 C '' I-J □ C 3 0 H 3 61N 7 0 2 546, 29 545.7 14-7 (-R; (R / CH3 Ch q C 3 0 H 3 6 r N 7 U 2 54 6, 2 9 54 5.7 14-8(S) iPr CH3 C32H40FN7O2 574.32 57 4 14-9! Laugh f '* _o IPr C32H40FN7O2 574.32 57 4.3 14-10(S} C '' I-J □ iPr C 3 2 H 4 0 FN 7 O2 574.32 574.3 14-11 R'i nPr CH3 C32H4 η FN 7O2 574.32 57 4 14-12{R-'i c ₂ h ₅ CH3 C31H ₃₈ FN7O ₂ 560.31 5 61 14-13(S) nPr H C31H ₃₈ FN ₇ O2 5 6 0.31 5 60, 3 14-14(S) nPr C '' I-J -j C32H40FN7O2 574.32 57 4 14-15(S/ nPr IPr C 3 4 H 4 4 Η N 7 0 2 602.35 602 14-16(S) G * Ί-Τ H C29H34FN7O2 532.28 5 32 _λ 2 14-17(s} C2H5 jtí C30H36FN7O2 5 4 6, 2 9 54 6.2
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 127/165
13 /
Ex.At the. * ** R ¹ R ¹² Formula [M + H] ⁺ calculated [M + H] ⁺ found 14-18(S) c ₂ h ₅ IPr C ₃₃ H ₄₂ F'N ₇ O ₂ 588.34 58 8.3 14-19(R) nPr iPr C ₃ 4 H 4 4 1 N 7 0 2 602.35 602.3 14 - 2 0(R / nPr TT C ₃ iH ₃ gFN ₇ C'2 560.31 5 60.3 J 4 - 2 J(R) iPr IPr C ₃ 4H44FN ₇ O ₂ 602.3 5 62 0.3 14-22! Laugh iPr jtí C ₃ iH ₃₈ FN ₇ O ₂ 560.31 560.3 14-23 (S} i re; iPr OH 3 03211403/11702 574.32 574.2 14-24 R'i R'i IPr CH3 N7O2 574.32 57 4.7
Table 15
Ex.At the. R ¹ R ¹³ R ¹² Formula [M + H] ⁺ calculated [M4-H] ⁺ found 15-1 ch ₃ H ch ₃ C27H ₃ oFN ₇ 0 ₂ 504.24 504 15-2 cPr u cPr 0 31H 3 4 E N 7 0 2 556.28 55 6, 3 15-3 ch ₃ Ch q ch ₃ 518.26 518 15-4 IPr H ch ₃ C ₂ 9.n. ₃ 4FN7O ₂ 532.28 532.2 15 "5 nPr jtí ch ₃ Ç . ₂ yH ₃ 4FN7O ₂ 532.28 532.2 1 6 C2H5 M ch ₃ C28H32FN7O2 518.26 517.8
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 128/165
114/136
Table 16
Ex.At the. R ¹ R ¹⁷ _R 1S Formula calculated [M4-H] ⁺ found 1 6-1 H - (Ç H ₂ ) 5- C ₂₇ H ₃ iFN ₆ O 475.25 475.1 16-2 ch ₃ ch ₃ tBu C ₂₈ H ₃₅ FN ₆ O 491.29 491.1 1 6 - 3 ch ₃  (Ç H ₂ ) 5 ”' C ₂₈ H ₃₃ FN ₆ O 489.27 489, 3 16-4 ch ₃ - (CH ₂ ) 2 0 (CH _{2 2} - C ₂₇ H ₃ iFN ₃ O ₂ 491.25 4 91, 1 16-5 ch ₃ ~ (c 4 2) 3 ~~ C ₂₆ H ₂₉ FN ₆ U 461.24 461.2 16-6 ch ₃ ch ₃ cpzopil C ₂₇ H ₃ iFN ₆ O / ^ 7 r n -d / O f z. 475, 1
Ex.At the. R ¹ γ-> 15 Formula [M + H] ⁺ calculated [M + H] ⁺ found 17-1 H f '* C ₂₃ H ₂₂ FN ₅ O ₃ 4 3 6, 17 4 3 6, 0 17-2 H cpropyl c ₂₅ h ₂₄ fn ₅ o ₃ 462.19 4 62 17-3 H (CH ₂ ) ₂ N (ch ₃ ) 2 c ₂₆ h ₂₉ fn ₆ o ₃ 493.23 4 93 17-4 H CH ₂ CH ₂ OH c ₂₄ h ₂₄ fn ₅ o ₄ 4 6 6, 18 4 66.1 17-5 H ⁴ ”'C 7 ^N ~ ^CH3 C ₂₈ H ₃ iFN ₆ O ₃ 519.24 519, 2
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15 /
Ex.At the. R ¹ R ¹⁵ Formula [M + H] ⁺ calculated [M + H] ⁺ found 17-6 HC ₂₇ H ₂₈ FN ₅ O ₄ 506.21 5 0 6, 1 17-7 Hc ₂₆ h ₂₇ fn ₆ o ₃ 491.21 4 91 17 - 8 H j f ~ {/ í ”) Ü2 8 ^ 30 504.23 5 Ω 5 17-9 HC ₂₆ H ₂₆ FN ₅ O ₄ 492.20 493 17-10 ch ₃ C ₂₉ H ₃₂ F ₂ N ₅ O ₃ 551.25 b 5 3 17-11 ch ₃ -1-- c ₂₇ h ₂₇ f ₂ n ₅ o ₄ 524.20 524.5 17-12 ch ₃ -J— / C ₂₇ H ₂₈ F ₂ N _s O ₃ 523.22 523
Table 18
F
Ex.At the.R ¹ X Formula [M + H] ⁺ calculated [M + H] 'found 18-1ch ₃ ch ₂ oh c ₂₃ h ₂₄ fn ₅ o ₂ 422.19 422.1 18-24- ^ ~ ^ n-ch ₃ ch ₂ oh c ₂₈ h ₃₃ fn ₆ 0 ₂ 505.27 505.2 18-3(CH ₂ ) ₂ nhch ₃ ch ₂ oh c ₂₅ h ₂₉ fn ₆ o ₂ 465.23 465.2 18 - 4 (R) -jZ n ~ ch ₃ ___ / ³ ch ₂ oh c ₂₈ h ₃₃ fn ₆ o ₂ 505.27 5 5
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Ex.At the. * R ¹ X Formula [M + H] ⁺ calculated [M + H] ⁺ found 18 ”5 I c \ /CH ₂ OH ClgHjaFNgCy 505.27 505 1 d ”b (R) (CH ₂ ) 2NHCH3 CH ₂ OH C25H29FN6O2 465.23 4 65 18 “1 (Sj (CH ₂ ) 2NHCH3 CH ₂ OH C25H ₂₉ FN ₆ O2 465.23 4 65
Table 19
Ex.At the. R ¹ Formula [MfH] ⁺ C3.1 cul cul [MfH] ⁺ found 19-1 F9j La'VA I n I.L HN-n N' X C ₃ iH _3S FN ₇ O ₂ 560.31 560 19-2 F HO ^ xk elk. x- 9 J (1 A ΪΙ 1 1 '! 1% Jk N k X hn-n n ^x 7 Ί C32H3SFN7O3 588.30 588 19-3 F ^HO vS O W <11 kl hn-n n - ^ - ^{x H} L_ / C32H38FN7O2 572.31 572, 1 19-4 FΗ C ₂₆ H27FN ₆ O2 475.22 475
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11 // 136
Ex.At the. R ¹ Formula [M + H] ⁺ calculated [M + H] ⁺ found 19-5 F9 sj Xjl aH C27H29tN ₅ O2 489.23 489 19-6 F9 'Jj' GO ! ! 1 / AzNkHN-NH C28H31FN6O2 5 0 3.2 5 503 19-7 F ^ho yS hn-n NH C27H29FN6O2 489.23 490 19-8 FHC Ã9 ‘ JL .NH L .0 hn-n C ₂₆ H ₂ 7FN ₅ O3 491.21 492 19-9 FHO.cXaihayããHN-NH C28H ₃₃ FNsO 489.27 4 8 9, 2 19-10 F HO ^ X V . JL. N. HN-N ^N H c ₂₈ h ₃₃ fn ₆ o 489.27 489.2
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Biological assays [00184] The compounds of the invention have been characterized in one or more of the following biological assays.
Assay 1: JAK kinase biochemical assays [00185] A panel of four LANThaScreen JAK biochemical assays (JAK1, 2, 3 and Tyk2) was performed in a common reaction buffer for, kinase (50 mm HEPES, pH 7.5, 0.01% Brij ~ 35, 10 mm mgC1.2, and 1 mm EGT.A). Recombinant GST-labeled JAK enzymes and the GFP-labeled STAT1 peptide substrate were purchased from Life Technologies.
[00186] Compounds with. Serial dilutions were preincubated with each of the four JAK enzymes and the substrate in 384 white well microplates (Corning) at room temperature for 1 h. ATP was added later to start the kinase reactions at 10 pL of total volume, with 1% DMSO. The final enzyme concentrations for JAK1, 2, 3 and Tyk2 are 4.2 nM, 0.1. nM, 1 nM, and 0.25 nM, respectively; the corresponding concentrations of Km ATP used are 25 μΜ, 3 μΜ, 1.6 μΜ and 10 μΜ; while the substrate concentration is 200 nM for all four assays. The kinase reactions continued for 1 hour at room temperature before adding a preparation of 10 μΐ of EDTA (10 mm final concentration) and Tb-anti-pSTAT1 antibody (pTyr701) (Life Technologies, 2 nM final concentration.) in TR-FRET dilution buffer (Life Technologies). The plates were incubated at room temperature for 1 hour before being read in the Envision reader (Perkin Elmer). The emission rate signals (52 0 nm / 495 nm.) Were recorded and used to calculate the percentage of inhibition values based on DMSO and background controls.
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119/136 [00187] For dose-response analysis, percent inhibition data were plotted against the concentrations of the compounds, and the IC ₅₀ values were determined from a robust 4-parameter fit model with the Prism software (GraphPad Software). The results were expressed as pICso (negative IC 50 logarithm) and subsequently converted to pK ± (negative dissociation constant logarithm, Ki) using the Cheng-Prusoff equation.
[00188] The test compounds with a lower K _L value or higher pKi in each of the four JAK assays show greater inhibition of JAK activity.
Assay 2: JAKI cell potency assay [00189] The AlphaScreen JAKI cell potency assay was done by measuring interleukin-13 induced STAT6 phosphorylation (IL-13, R&D Systems) in. human lung epithelial cells BEAS-2B (ATCC). The anti ~ STAT6 antibody (Cell Signaling Technologies) was conjugated to AlphaScreen acceptor beads (Perkin Elmer), while the anti-pSTATo antibody (pTyr641) (Cell Signaling Technologies) was biotinylated using the sulfo-NHS-biotin EZ-Link (Thermo Scientific).
[00190] BEAS-2B cells were cultured at 37 ° C in a humidified incubator at 5% CO2 in 50% DMEM / 50% F medium (Life Technologies), supplemented with 10% FBS (Hyclone), 100 U / ml of penicillin, 100 µg / ml of streptomycin (Life Technologies), and 2 mm of GlutaMAX (Life Technologies). On day 1 of the assay, cells were seeded at a density of 7,500 cells / well in. white plates with 384 wells coated with. poly-D-lysine (Corning) with 25; iL of medium, and stayed overnight in the incubator for adherence.
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On day 2 of the assay, the medium was removed and replaced with pL of assay buffer (Hank's Balanced Salt Solution, 25 mm. HEPES and 1 mg / ml of bovine serum alburaine / BSA) containing dose responses of the test compounds. The compounds were serially diluted in DMSO and then diluted 1000 times in the media to reach the final concentration of 0.1% DMSO. The cells were incubated with the test compounds at 37 ° C for h, followed by the addition of 12 μΐ of pre-heated IL-13 (80 ng / ml in assay buffer) for stimulation. After incubation at 37 ° C for 30 minutes, the assay buffer (containing compound and IL-13) was removed, and 10 μΐ of cell lysis buffer (25 mm HEPES, 0.1% SDS, 1% NP-40, 5 mm mgCÍ2, 1.3 mm, 1 mm EDTA, EGTA and supplement with Roche Diagnostics Ultra mini and PhosSTOP protease inhibitors). The plates were shaken at room temperature for 30 minutes before adding the detection reagents. A mixture of anti-biotin-pSTAT6 and anti-STAT6 conjugated acceptor beads was added first and incubated at room temperature for 2 h, followed by the addition of donor beads conjugated to streptavidin (Perkin Elmer). After one. minimum incubation h, assay plates were read on the EnVision plate reader. AlphaScreen luminescence signals were recorded and used to calculate percentage inhibition values based on DMSO and background controls.
[00191] For dose-response analysis, percent inhibition data were plotted against compound concentrations, and IC50 values were determined from a robust 4-parameter fit model with. the Prism program. The results can also be expressed as the negative logarithm of the IC50 value, pICso.
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121/136 [00192] The test compounds with the lowest ICsc value of the highest pICso in that assay showed greater inhibition of IL-13-induced STAT6 phosphorylation.
Results of the in vitro test [00193] The selected compounds of the invention were tested in the four assays of the enzyme JAK1; JAK2, JAK3 and Tyk2 and the BEAS-2B cell potency assay described above. As shown in Table 19 below, it was observed that the enzymatic potency of JAK1 was predictive of both pan-JAK enzymatic activity and the cellular potency in the BEASTS assay. Therefore, all compounds were tested in the JAK1 enzyme assay and the BEAS-2B cell assay and most were also tested in the JAK3 enzyme assay. All compounds exhibited K ± values of JAK1 between 0.04 nM and 0.6 nM (pKi between 9.2 and 10.4). The compounds tested in the JAK3 enzyme assay showed Ki values between 0.08 nM and 0.5 nM (pK _± between 9.3 and 10.1). The tested compounds showed IC 50 values displayed in the BEAS-2B assay between 3> nM and 100 nM (pICso between 7 and 8.5).
Table 20
Example number LJAJC1Ki(nM) JAK2Ki(nM) JAK3Ki(nM) Tyk2Ki(nM) BE AS — 2 BIC50(nM)0.05 0.02 0.08 0.25 5.0 O 0.06 0.02 0.10 0.40 5, 9 5 0, 0 80.206, 5 6 0 f 100.254.2 7 0, 0 6O / j-65, 4 1-2 0.05 0.03 0 _f 1 6 0.08 7.8 1-12 0, 0 6 0.06 0.25 0.25 5, 9
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1-37 0 f 10 0.04 0.50 1.2 6 % Ci 2-15 0, 0 60.164.0 9-21 0, 0 6 0.03 0.25 1.0 0 4.0 12-14 0.060.23 _f 3
Test 3: Plasma and lung pharmacokinetics in mice [00194] The plasma and lung levels of the tested compounds and their proportions were determined as follows. BALB / c mice from Charles River Laboratories were used in the trial. The tested compounds were individually formulated in 20% propylene glycol in citrate buffer with pH 4 in one. The concentration of 0.2 mg / ml and 50 µL of the administered solution was introduced into the trachea of a mouse by aspiration. At various times (usually 0.167, 2, 6, 24 h) post-administration, blood samples were taken through cardiac puncture and intact lungs were taken from the rats. Blood samples were centrifuged (Eppendorf centrifuge, 5804R) for 4 minutes at approximately 12,000 rpm at 4 ° C to collect plasma. The lungs were dry padded, weighed and homogenized in a 1: 3 dilution in. sterile water. The plasma and lung levels of the test compound were determined by LC-MS analysis against analytical standards constructed on a standard curve in the test matrix. A lung-to-plasma ratio was determined as the ratio of lung ASC in pg hr / ml to plasma ASC in ug hr / ml, where ASC is conventionally defined as the area under the test compound versus time curve. The compounds of the invention exhibited exposure in the lung from one to
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123/136 two orders of magnitude greater than plasma exposure. All compounds profiled in this test had a half-life between about 4.5 and about 14 noras.
Assay 4: Murine (Mouse) Model of IL-13-Induced pSTAT6 Induction in Lung Tissue [00195] IL-13 is an important cytokine underlying the pathophysiology of asthma (Kudlacz et al. Eur. J. Pharmacol, 2008, 582,154- 161). IL-13 binds to cell surface receptors by activating members of the Janus family of kinases (JAK) which then phosphorylate STAT6 and subsequently activate transcription pathways. In the model described, a dose of IL-13 was delivered locally to the mouse lungs to induce phosphorylation of STAT6 (pSTAT6) which is then measured as the end point (result).
[00196] Harlan adult balb / c mice were used in the trial. On the study day, the animals were lightly anesthetized with isoflurane and received administration of test vehicle or compound (1 mg / ml, 50 pL of total volume over several breaths) via oral aspiration. The animals were placed in the lateral decubitus post-dose and monitored for. full recovery from anesthesia before they are returned to their cage. Four hours later, the animals were again anesthetized briefly and challenged with either vehicle or IL-13 (0.03 pg total dose released, 50 pL total volume) via oral aspiration before being monitored during recovery from anesthesia and returned to your cage. One hour after the administration of IL-13 or vehicle, the lungs were collected for detection of pSTAT6 using an anti-STAT6 ELISA (rabbit mAb capture / coat antibody; antibody
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124/136 mouse mAb detection / capture: anti-pSTAT6-pY641; secondary antibody: anti-mouse IgG-HRP) and analyzed for the total drug concentration as described above in assay 3.
[00197] The selected compounds of the invention were tested in the assay. The activity in the model is evidenced by a decrease in the level of pSTATE present in the lung of the animals treated in 5 hours in. compared to vehicle-treated animals, controls challenged with IL-13. A. difference between control animals that were treated with vehicle, challenged with IL-13 and control animals that were treated with vehicle, challenged with vehicle dictated 0 to 100% inhibitory effect, respectively, in any experiment. Exemplary compounds of the invention were tested in the assay and exhibited inhibition of STA.T6 phosphorylation 4 hours after challenge with IL-13 as documented below. The 9-22 compound was observed as an exception under the conditions of the assay.
[00198] By confirming the relevance of the JAK-STAT pathway in airway inflammation, compounds that demonstrated target involvement in vivo in the IL-13-induced pSTA_T6 mouse model were subsequently tested and found to be effective in a mouse model of eosinophilic inflammation induced by allergens.
Results of the in vivo test [00199] The selected compounds of the invention were characterized in the pharmacokinetic test (Test 3) and pharmacodynamic test (Test 4). A good correlation was observed between the concentration of the test compound in the lung determined in the pharmacokinetics test and in the pharmacodynamics test
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125/136 was a similar therapy dosage post-administration. Observation of the significant compound concentration in the mouse lung in the pharmacodynamic assay confirmed that the observed inhibition of IL-13-induced pSTAT6 induction was a result of the activity of the test compound.
[00200] In the table below, for the ratio of lung exposure to plasma exposure (Assay 3), A denotes a ratio 100-200, B denotes a ratio between 50 and 100 and C denotes a ratio between 20 and 50. For the percent inhibition of IL-13-induced pSTAT6 induction (Assay 4), A represents between 60% and 80% inhibition, B represents between 40% and 60% inhibition and C represents between 25% and 40% inhibition.
Table 21
Example number Plasma lung ratio3 Inhibition of pSTAT6Essay 4 _L THE THE 3 B B 5 B lZj 6 B izi 7 B n 1 -18 B THE 1-28 Ç B 1-29 Ç THE Ί ... β 7ç 2-2 ç B 2 - 3THE 2-8 ç P 2-9 ç ç
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Example number Lung ratio -plasmaEssay 3 Inhibition of pSTAT6Essay 4 2-15B 3-2 B Ά 3-5THE 3-24ç 5-8 Ç THE. 9-3 THE lZj 9-21iZÍ » 12-8THE 12-14 B n 13-2 THE. THE 13-5 THE. U 13-6 B B
Test 5: Murine model of eosinophilic inflammation of the lung induced by Alternaria alternata [00201] Eosinophilia of the airways is a hallmark of human asthma. Alternaria alternata is a fungal aeroallergen that can aggravate asthma in humans and induce eosinophilic inflammation in rat lungs (Havaux et al. Clin Exp Immunol. 2005 Feb; 139 (2): 179-88). In mice, Alternaria has been shown to indirectly activate type 2 innate lymphoid cells resident in the lung, to which they respond (eg, IL-2 and IL-7) and release cytokines cypendent es ci eu Ar (, p eg, .1 n — 5 and .i. L - 1 o) echo or ci enara eosinophilic inflammation (Bartemes et al. J Immunol. 2012 Fe
1; 188 (3): 1503-13).
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12 // 136 [00202] Tacocnic seven to nine week old C57 mice were used in the study. On the study day, the animals were lightly anesthetized with isoflurane and received administration of the vehicle or test compound (0.03-1.0 mg / ml, 50 pL of the total volume over several breaths) via oropharyngeal aspiration. The animals were placed in. lateral post-dose decubitus and monitored for full recovery from anesthesia before being returned to their cage. One hour later, the animals were briefly anesthetized again and challenged with Alternaria vehicle or extract (200 pg of total released dose, 50 pL of total volume) through oropharyngeal aspiration before being monitored during recovery from anesthesia and returned to their cage . Forty-eight hours after Alternaria administration, bronchoalveolar lavage fluid (BALF) was collected and eosinophils were counted in the BALF using the Advia 120 Hematology System (Siemens).
[00203] The selected compounds of the invention demonstrating activity in vivo in the IL-13-pSTAT6 pharmacodynamic assay were tested in the Alternaria assay. The activity in the model is evidenced by a decrease in the level of eosinophils present in the BALF of animals treated in 48 hours compared to animals treated with vehicles, controls challenged with Alternaria. Data are expressed as a percentage, inhibiting the response of vehicle-treated BALF eosinophils and challenged with Alternaria. To calculate the percentage of inhibition, the number of BALF eosinophils for each condition was converted to. percentage, of the average vehicle-treated BALF eosinophils, challenged with Alternaria and subtracted from 100%. Exemplary compounds
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128/136 of the invention were tested in the assay and exhibited BALF eosinophil count inhibition 48 hours after challenge with
It would alternate as documented below.
Results of the in vivo test [00204] All compounds tested demonstrated a range of inhibition (73% - 93%) of BALF eosinophils induced by Alternaria. The following table reflects the maximum percentage of the statistically significant inhibition of the level of eosinophil induction in vehicle treated, challenged with Alternaria.
Table 22
Number ofexample Percentage of BALF inhibition and eosinophils induced by Alternaria9093 6 7 3 /
Test 6: IL-5-mediated eosinophil survival test [00205] The potency of the test compound for IL-5-mediated eosinophil survival was measured in human eosinophils isolated from human blood (AllCells). As IL-5 signals via JAK, this assay provides a measure of JAK cell potency.
[00206] Human eosinophils have been isolated from fresh human blood (AllCells) from healthy donors. The blood was mixed with. 4.5% Dextran (Sigma-Aldrich) in. a 0.9% chloride solution (Sigma-Aldrich). the cells
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129/136 red bloods were left to rest for 35 minutes to settle. The leukocyte-rich top layer was removed and superimposed on Ficoll-Paque (GE Healthcare) and centrifuged at 600 g for 30 minutes. The plasma and layers of mononuclear cells were removed before the granulocyte layer was lysed with water to remove any contamination of red blood cells. The eosinophils were subsequently purified using a human eosinophil isolation kit (Miltenyi Biotec). A fraction of the purified eosinophils was incubated with anti-CD16 FITC (Miltenyi Biotec) for 10 minutes at 4 ° C in the dark. Purity was analyzed using a. flow cytometer LSR1I (BD Biosciences).
[00207] The cells were cultured at 37 ° C, in a 5% CO ₂ humidified incubator in RPMI 1640 (Life Technologies), supplemented with 10% Heat Inactivated Bovine Fetal Serum (SUB, Life Technologies), 2 mm. Glutamax (Life Technologies), 25 mm HEPES (Life Technologies) and IX Pen / Strep (Life Technologies). The cells were seeded in 10,000 cells / well in the media (50 μΐ). The plate was centrifuged at 300 g for 5 minutes and the supernatant was removed. The compounds were serially diluted in DMSO and then diluted 500 times to a final test concentration of 2x in the media. Test compounds (50 g / well) were added to the cells and incubated at 37 ° C, 5% CO ₂ for 1 hour, followed by the addition of IL-5 (R&D Systems; final concentrations of 1 ng / ml and 10 ng / ml) in pre-heated (50 pi) test media for / 2 hours.
[00208] After cytokine stimulation, the cells were centrifuged at 300 g for 5 minutes and washed twice
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130/136 times with cold DPBS (Life Technologies). To access viability and apoptosis, cells were incubated with. propidium iodide (Thermo Fisher Scientific) and APC Annexin V (BD Biosciences) and analyzed using an LSRII flow cytometer (BD Biosciences). IC 50 values were determined from the analysis of percent cell viability versus compound concentration curves. The data were expressed as pICso values (IC50 negative decadic logarithm). Example 1 compound had a pICso value of 7,310.4 in the presence of 10 pg / ml IL-5 and one. pICso value 5,710.1 in the presence of 1 ng / ml IL-5.
Assay 7: JAK cell potency assay: IFNy-stimulated IL-2 / anti-CD3 inhibition in human PBMCs [00209] The potency of the test compound for inhibiting interleukin-2 (IL-2) / stimulated anti-CD3 by the interferon range (IFNy) was measured in peripheral blood mononuclear cells (PBMCs) isolated from whole human blood (Stanford Blood Center). As IL-2 signals through JAK, this assay provides a measure of JAK cell potency.
[00210] (1) Peripheral blood mononuclear cells (PBMC) were isolated from whole human blood from healthy donors using a Ficoll gradient. The cells were cultured at 37 ° C, in a humidified incubator at 5% CO2 in RPMI (Life Technologies), supplemented with 10% Heat Inactivated Bovine Fetal Serum (SFB, Life Technologies), 2 mm Glutamax (Life Technologies) , 25 mm HEPES (Life Technologies) and IX Pen / Strep (Life Technologies). The cells were seeded in 200,000 cells / well in the media (50 µl) and cultured for 1 h. The compounds were serially diluted in DMSO and then diluted 500 times (to a
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131/136 final test of 2x) in the media. The test compounds (100 µL / well were added to the cells and incubated at 37 ° C, 5% CO ₂ for 1 hour, followed by the addition of IL-2 (R&D Systems; final concentration 100 ng / ml) and anti -CD.3 (BD Biosciences; final 'concentration of 1 pg / ml) in preheated test media (50 pl) for 24 hours.
[00211] (2) After cytokine stimulation, the cells were centrifuged at 500 g for 5 minutes and the supernatant removed and frozen at -80 ° C. To determine the inhibitory potency of the test compound in response to IL2 / anti-Cd3, concentrations of IFNy supernatant were measured via ELISA (R&D Systems). IC50 values were determined from the analysis of the IFNv concentration curves versus the concentration of the compounds. The data were expressed as pIC ₅₀ values (IC ₅₀ negative decadic logarithm). The compound of Example 1 exhibited a pICso value of about 6.9 in that assay. The compound of Example 3 exhibited a pICso value of about 7.2 in that assay. The compound of Example 1-37 exhibited a pICso value of about 7.2 in that assay.
Assay 8: JAK cell potency assay: inhibition of IL-2-stimulated pSTATS in CD4 + T cells [00212] The potency of test compounds for inhibiting STAT5 phosphorylation stimulated by interleukin-2 (IL-2) / anti -CD3 was measured in. CD4 positive T cells (CD4 +), peripheral blood mononuclear cells (PBMCs) isolated from whole human blood (Stanford Blood
Senter) using flow cytometry. As IL-2 signals through JAK, this assay provides a measure of JAK cell potency.
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132/136 [00213] CD4 + T cells were identified using an anti-CD4 antibody conjugated with phycoerythrobilin (PE) (Clone RPA-T4, BD Biosciences), while an Alexa Fluor 647 conjugated anti-pSTAT5 antibody (pY694, Clone 47 , BD Biosciences) to detect STAT5 phosphorylation.
[00214] (1) The protocol of paragraph 7 of the trial (1) was followed with the exception that stimulation of cytokines with anti-CD3 was performed for 30 minutes instead of 24 h.
[00215] (2) After cytokine stimulation, cells were fixed with preheated fixation solution (200 µL; BD Biosciences) for 10 minutes at 37 ° C, 5% CO2, washed twice with. DPBS buffer (1 ml, Life Technologies), and resuspended in cooled Perm III buffer (1000 pL, BD Biosciences) for 30 minutes at 4 ° C. The cells were washed twice with 2% SFB DPBS (FACS buffer) and then resuspended in FACS buffer (100 pL) containing anti-CD4 PE (1:50 dilution) and anti-CD3 antiCD3Alexa Fluor 647 (1: 5 dilution) for 60 minutes at room temperature in the dark. After incubation, the cells were washed twice with. FACS buffer before being analyzed using the LSRII flow cytometer (BD Biosciences). To determine the inhibitory potency of the test compounds in response to IL ~ 2 / anti - CD3, the median fluorescent intensity (IFM) of pSTAT5 in CD4 + T cells was measured. IC50 values were determined from the analysis of the IMF inhibition curves versus compound concentration. Data were expressed as pIC.50 values (IC50 negative decadic logarithm). The compound of Example 1 exhibited a pICso value of about 7.3 in that assay. The compound of Example 3 exhibited a pICso value of about
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7.7 in this essay. The compound of Example 1-37 exhibited a p 1C value of about 5, 1 ne s s and en a s.
Assay 9: JAK cell potency assay: inhibition of IL-4-stimulated pSTAT6 in CD3 + T cells [00216] The potency of the test compound for inhibiting STAT6 phosphorylation stimulated by interleukin-4 (IL4) was measured in. CD3-positive T cells (CD3 +), peripheral blood mononuclear cells (PBMCs) isolated from whole human blood (Stanford Blood Center) using flow cytometry. As IL-4 signals via JAK, this assay provides a measure of JAK cell potency.
[00217] CD3 + T cells were identified using an anti-CD3 antibody conjugated to phycoerythrobilin (PE) (UCHT1 Clone, BD Biosciences), while an Alexa Fluor 647 conjugated anti-pSTAT6 antibody was used (pY641, Clone 18 / P, BD Biosciences ) to detect STAT6 phosphorylation.
[00218] Peripheral blood mononuclear cells (PBMC) were isolated from whole human blood from healthy donors as in Assays 7 and 8. The cells were seeded at the rate of 250,000 cells / well in media (200 pL), grown for 1 h then resuspended in assay media (50 µl) (RPMI supplemented with 0.1% bovine serum albumin (Sigma), 2 mm Glutamax, 25 mm HEPES and IX Penstrep) containing different concentrations of test compounds. The compounds were serially diluted in DMSO and then diluted 500 times (to a final test concentration of 2x) in the test media. Test compounds (50 μι) 1 'were incubated with cells at 37 ^J C, 5% CO ₂ for 1 h, followed by the addition of IL-4 (50 μΐ) (R&D Systems; final concentration 20 ng / ml) in pre-trial medium Attorney's Guide No .: P-338-PCT
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134/136 heated for 30 minutes. After stimulation of cytokines,
the cells(100 uL) They were, fixed with. preheated fixation solution (BD Biosciences) for 10 minutes at 37 ° C, 5% CO ₂ , washed twice with FACS buffer (1 ml) (2% DPBS FBS) and
resuspended in ice-cold Perm III buffer (1000 pL) (BD Biosciences) for 30 minutes at 4 ° C. The cells were washed twice with FACS buffer and, in. then placed on. suspension of FACS buffer (100 uL) containing anti ~ CD3 PE
(dilution 50ο 1:50) and anti-pSTAT6 Alexa Fluor 647 (dilution 1: 5) during 60 minutes at room temperature in the dark. After incubate the cells were washed twice with. plug FACS ant : to be analyzed using the flow cytometer LSRII (I 3D Biosciences).
[00219] To determine the inhibitory potency of the test compound in response to IL-4, the
intensic median fluorescent iade (IFM) of pSTAT6 in cells T CD3 +. IC50 values were determined from the analyze of IMF inhibition curves versus concentration
the compounds. The data were expressed as pICso (IC50
logaritr negative decadic). The compound of Example 1 exhibited one go01 pICso r of about 7.7 in that assay. The compound of Example 3 exhibited a pICso value of about 8 in that test. The compound of Example 16-4 exhibited a pICso value
of about 8.1 in that trial.
Assay 10: JAK cell potency assay: inhibition of pSTAT3 stimulated by IL- ~ 6 in CD3 + T cells [00220] A protocol similar to Assay 9 was used
to det seminar the power of the test compound to
inhibition of STAT3 phosphorylation stimulated by interleukin 6 (IL-6). An anti ~ STAT3 antibody was used
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 149/165
135/136
Alexa Fluor 647 conjugate (ρΥ / 05, Clone 4 / P, BD Biosciences) to detect phosphorylation of STAT3. The compound of Example 1 ex u i u ur va 1 o r cte p Σ C 50 0 and about 7.2 ne s e n e s to i 0. The compound of Example 3 exhibited a pICso value of about 7.4 in that assay.
Crystalline structure [00221] A crystalline structure of the compound of Example 2 (an analogue of the compounds disclosed herein which was disclosed in the provisional application for which this application has priority) was obtained bound to human JAK1 in a resolution of 2.28 Å. The ligand was observed to bind to the ATP binding site. Seven specific hydrogen bridges have been identified based on a distance of 3.5 A or less between the donor and recipient atoms. Of special note, a hydrogen bridge has been identified between the carbonyl of the exocyclic starch of the compound of Example 2 and the Arg879 side chain of JAK1. In previous modeling studies, this interaction has been proposed as a way to provide selectivity for JAK1 over other tyrosine kinases, because otherwise, closely related kinases (eg, TRKA, VEGFR, ABL1) do not have one. arginine residue at the equivalent location. The observed results of the hydrogen bridge in the crystalline structure and improved kinome selectivity compared to the series that does not have exocyclic starch validate the hypothesis of this design.
[00222] Although the present invention has been described with reference to specific aspects or its modalities, those who are skilled in the art will understand that several changes can be made or equivalent can be replaced, without departing from the true spirit and spirit.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 150/165
136/136 scope of the invention. In addition, to the extent permitted by applicable statutes and applicable patent regulations, all publications, patents and applications cited herein are incorporated by reference in their entirety, as if each document had been incorporated individually by reference.

权利要求:
Claims (13)
[1]
WHAT IT CLAIMS IS:
A compound of the formula (I):

[2]
2/13
Bf ³ and R ^{± 4} were combined with the nitrogen atom to which they are attached form morpholinyl, or
R ¹³ is R ⁸ and R ¹⁴ is R ⁹ ,
R ³ is selected from hydrogen, CI - ₃ alkyl and C3scycloalquil, CI - ₃ alkyl which is optionally substituted by -OH , or -OCi-salquil,
R ⁴ is C1-3alkyl, where C1-3alkyl is optionally substituted by -OH,
R ³ and R ⁶ are independently CI_ ₃ alkyl or R ° and R ⁶ together with the nitrogen atom to which they are attached form a. 5- or 6-membered heterocycle, optionally including an oxygen atom,
R is Cl - ₃ alkyl, optionally substituted by a 5- or 6 - membered ring containing one nitrogen atom,
R ⁸ is hydrogen or C1-3alkyl,
R ⁹ is selected from - (CH ₂ ) ₂ NR ° R ^1] · or a 4- to 6-membered heterocycle containing a nitrogen atom, where a nitrogen atom is replaced by R ¹² ,
R ^1U and R ¹ are independently C1-3alkyl or R ^lü and R ¹¹ together with the nitrogen atom to which they are attached form a 5- or 6-membered heterocycle,
R ^z is ^± C1-3alqu.il or Ca-scicloalquil, CI - ₃ alkyl which is optionally substituted by -ΌΗ,
R ¹³ is selected from Ci-salquil, C ₃ ecicloalkyl and a 5- or 6-membered heterocycle, including a heteroatom selected from nitrogen and oxygen, where Ci-3alkyl is optionally substituted by -OH or -N (Ci- 3alkyl) 2, and a heterocycle
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 153/165
[3]
3/13 and 5 or 6 members is optionally replaced by Cisa lqu.il,
R ¹⁶ is if learned from -nr ¹⁷ r ¹⁸and -OR ¹⁹ , R ¹⁷ and R ¹° are independently and _C-3 or C 4alquil scicloalkyl or R ' ^{l /} and R ¹⁸ together I'm with atom of
nitrogen to which they are attached form a heterocycle or a 5 6 membered optionally including an oxygen atom, where the heterocycle is optionally substituted by Cl - ₃ alkyl,
R ^iy, R ^20, R ^2i, R ^22, R ^z3 and R ^z4 are independently from CI seiecionaoos alqurl _3, or. pharmaceutically acceptable salt
The compound of Claim 1, where R ¹ is selected from hydrogen and C1-3 alkyl, and X is selected from -C (0) R ² and -CH2R ¹⁶ .
The compound of Claim 2, where
R * θ ° r.:4 _{Cori un} r j _{with the} nitrogen atom to which they are attached form a monocyclic heterocyclic bicyclic C 6 or 7 - membered ring containing an additional nitrogen atom, where the additional nitrogen atom is replaced by R ³ and the heterocycle is optionally substituted by one or two R ⁴ or
R ^{± J} and R ¹⁴ together with the nitrogen atom to which they are attached form a 5- to 6-membered heterocycle, where the heterocycle is optionally substituted by -NR ³ R ⁶ and R /, or
R ¹³ is R ⁸ and R ¹⁴ is R ⁹ ,
^J R is selected from hydrogen and Cl - ₃ alkyl CI_ ₃ alkyl which is optionally substituted by -OH,
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 154/165
[4]
4/13
R ³ and R ^D are independently Ci-. ₃ alkyl or R ° and R ^c together with. the nitrogen atom to which they are attached forms one. 5- or 6 - membered · R 'is a C 3 alqu.il, opc.ionalm.ente pirroüdinil substituted by R ⁸ is C 3alquil, ^R9 is (CH2) 2NR ⁱ⁰ R ¹¹ or piperidinyl, in which piperidini) is substituted on the nitrogen atom by R ^1Z , R ^{; 0} and R ^{; 1} are C1-3alkyl independently, R ⁱ² is Ci-salkyl or Cs-gcycloalkyl, where Ci-salkyl is optionally substituted by —0H, R * ° is selected from Ci-salquil, C3cycloalkyl and a 5- or 6-membered heterocycle including a heteroatom selected from nitrogen and oxygen, where C- ₃ alkyl is optionally substituted by -OH and a 5- or 6-membered heterocycle is optionally substituted by Ci-salquil, R ¹⁶ is selected from -NR ^; 'R ¹⁸ and R ^z ' and R ^{± 8} are independently Ci-4alkyl or C3cycloalkyl or R ⁱ⁷ and R ⁱ⁸ together with the nitrogen atom to which they are attached form morpholinyl or a 5- or 6-membered heterocycle where the heterocycle is optionally replaced by Ci-salquil. The compound of Claim 3, where X is -C (O) R ² , Where R ² is -NR ¹³ R ¹⁴ . The compound of Claim 3, where X is -C (O) R ² , n. The compound of Claim 3, wherein X is -CH ₂ R ¹⁶ . The compound of Claim 1, wherein R ¹ is
selected from - (0¾) 2NR ^z0 R ²¹ and a heterocycle of
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 155/165
4- to 6 members containing a nitrogen atom, where a nitrogen atom is optionally substituted by R ^2z and is selected from ~ CH ₂ OR ²³ and -C (O) OR ²⁴ .
The compound of Claim 7, wherein R ¹ is selected

[5]
5--, R 'is hydrogen or C ₃ alkyl,
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 156/165
[6]
6/13
R ^s is ~ CH ₃ ,
R ⁹ is ~ (CH ₂ ) ₂ NR ^1o R ¹¹ or

[7]
7/13 ((S) -3- (dimethylamino) pyrrolldin-1-yl) ((S) -5-ethyl-2- (6 (2-ethyl-5-fluoro-4-hydroxyphenyl) -1H-indazole- 3-yl) -4,5,6,7tetra.hydro-3.H-imidazo [4,5-c] pyridin-6-yl) methanone, (5) - (2- (6- (2-ethyl- 5-fluoro-4-hydroxyphenyl) -1H-indazol-3 yl) -5 ~ isopropyl-4,5,6,7-tetrahydro-3.fi-imidazo [4,5-c] pyridin-
6-yl) (4-methyl-1,4-diazepan-1-yl) methanone, ((5) -2- (6- (2-ethyl-5-fluoro-4-hydroxyphenyl) -lif-indazol3-yl ) -5-methyl ~ 4,5,6,7-tetrahydro ~ 3H- imidazo [4,5-c] pyridin-611) ((A) -4- (2-hydroxyethyl) -2-methyl-piperazin-1 -yl) methanone, (5) - (2- (6- (2-ethyl-5-fluoro-4-hydroxyphenyl) -IH-indazol3-yl) -5-propyl ~ 4,5,6,7-tetrahydro- 3.H-im.ida.zo [4,5-c] pyridin-6 yl) (4- (2-hydroxyethyl) piperazin-1-yl) methanone, and their pharmaceutically acceptable salts.
The compound of Claim 9, wherein the compound is ((5) ~ 2,4-dimethylpiperazin-1-yl) ((S) -2- (6- (2-ethyl-5-fluoro-4 hydroxyphenyl) - lif-indazol-3-yl) -5-m.ethyl-4,5,6,7-tetrahydro-3.H imidazo [5,4-c] pyridin-6-yl) methanone or an acceptable salt from the pharmacist view.
The compound of Claim 9, wherein the compound is (R) -N- (2- (diethylamino) ethyl) -5-ethyl-2- (6- (2-ethyl-5-fluoro-4hydroxyphenyl) -lff- indazol-3-yl) -N-methyl-4,5,6,7-tetrahydro-3.H imidazo [5,4-c] pyridine-6-carboxamide or a pharmaceutically acceptable salt.
16. ((S) -2- (6- (2-ethyl-5-flu.orO-4- hydroxyphenyl) -1Hindazol-3-yl) -5-propyl-4,5,6,7-tet. .raid.ro-3H-imidazo [4,5c] pyridin-6-yl) ((IS, 4S) -5-methyl-2,5-diazabicyclo [2.2.1] heptan-2-yl) methanone of the formula
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 158/165
[8]
8/13

[9]
9/13

[10]
10/13 hypereosinophilic syndrome, Löffler's syndrome, correct bronchiolitis obliterans, organizational pneumonia, graft-versus-host disease, or pneumonitis induced by immunological checkpoint inhibitor.
24. A compound of any one of Claims 1 to
1 / for use in the treatment of lung transplant rejection in one. mammal.
25. The lung transplant compound · is organizing pneumonia,
Claim 24, where rejection of primary graft dysfunction, acute rejection, lymphocytic bronchiolitis or chronic pulmonary graft dysfunction.
26. The compound of Claim 24, wherein the lung transplant rejection is acute lung transplant rejection.
27. The compound of Claim 24, wherein the lung transplant rejection is chronic pulmonary graft dysfunction.
28. The compound of Claim 24, wherein the lung transplant rejection is bronchiolitis obliterans, restrictive chronic pulmonary graft dysfunction or graft neutrophil dysfunction.
29. Use of a compound from any of the
Claims 1 to 17 for the production of a medicament for the treatment of a respiratory disease in. a mammal.
30. The use of Claim 29, where the respiratory disease is asthma, chronic obstructive pulmonary disease, cystic fibrosis, pneumonitis, idiopathic pulmonary fibrosis, acute lung injury, acute respiratory distress syndrome, bronchitis, emphysema, bronchiolitis obliterans, or sarcoidosis.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 161/165
[11]
11/13
31. The use of Claim 30, wherein the respiratory disease is asthma or chronic obstructive pulmonary disease.
32. The use of Claim 29, wherein the respiratory disease is an eosinophilic disease, helminth infection, pulmonary arterial hypertension, lymphangioleiomyomatosis, bronchlectasis, an infiltrative lung disease, drug-induced pneumonitis, fungal-induced pneumonitis, allergic bronchopulmonary aspergillosis, pneumonitis, allergic bronchopulmonitis, pneumonitis hypersensitivity, eosinophilic granulomatosis with polyangiitis, idiopathic acute eosinophilic pneumonia, idiopathic chronic eosinophilic pneumonia, hypereosinophilic syndrome, Loffler syndrome, obliterating bronchiolitis with organizing pneumonia, graft-versus-host disease, and inhibitor-induced pneumonitis.
33. Use of a compound of any one of Claims 1 to 17 for the production of a medicament for the treatment of lung transplant rejection in a mammal.
34. The use of Claim 33, where lung transplant rejection is primary graft dysfunction, organizing pneumonia, acute rejection, lymphocytic bronchiolitis or chronic lung graft dysfunction.
35. The use of Claim 33, where the rejection of the lung transplant is acute rejection of the lung transplant.
36. The use of Claim 33, where lung transplant rejection is chronic lung graft dysfunction.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 162/165
[12]
12/13
37. The use of Claim 33, wherein the lung transplant rejection is bronchiolitis obliterans, restrictive chronic pulmonary graft dysfunction or graft neutrophil dysfunction.
38. A method of treating a respiratory disease in a mammal, the method comprising administering to the mammal a compound of any one of Claims 1 to 17 and a pharmaceutically acceptable carrier.
39. The method of Claim 38, wherein the respiratory disease is asthma, chronic obstructive pulmonary disease, cystic fibrosis, pneumonitis, idiopathic pulmonary fibrosis, acute lung injury, acute respiratory distress syndrome, bronchitis, emphysema, obliterating bronchiolitis, or sarcoidosis.
40. The method of Claim 38, wherein the respiratory disease is asthma or chronic obstructive pulmonary disease.
41. The method of Claim 38, wherein the respiratory disease is an eosinophilic disease, a helminth infection, pulmonary arterial hypertension, lymphangioleiomyomatosis, bronchiectasis, an infective lung disease, drug-induced pneumonitis, fungal-induced pneumonitis, allergic bronchopulmonary aspergillosis, pneumonitis, allergic bronchopulmonary pneumonitis hypersensitivity, eosinophilic granulomatosis with polyangiitis, idiopathic acute eosinophilic pneumonia, idiopathic chronic eosinophilic pneumonia, hypereosinophilic syndrome, Löffler syndrome, bronchiolitis obliterans with organizing pneumonia, graft-versus-host disease or immunological checkpoint inhibitor-induced pneumonitis.
Lawyer No .: P-338-PCT
Petition 870190088769, of 09/09/2019, p. 163/165
[13]
13/13
42. A method of treating lung transplant rejection in a mammal, the method comprising administering to the mammal a compound of any one of Claims 1 to 17 and a pharmaceutically acceptable carrier.
43. The method of Claim 42, wherein the lung transplant rejection is primary graft dysfunction, organizational pneumonia, acute rejection, lymphocytic bronchiolitis or chronic graft lung dysfunction.
44. The method of Claim 42, wherein the lung transplant rejection is acute lung transplant rejection.
45. The method of Claim 42, wherein the respiratory disease is chronic pulmonary graft dysfunction.
46. The method of Claim 42, wherein the lung transplant rejection is bronchiolitis obliterans, restrictive chronic pulmonary graft dysfunction or graft neutrophil dysfunction.

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法律状态:
2021-10-19| B350| Update of information on the portal [chapter 15.35 patent gazette]|

优先权:

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申请号 | 申请日 | 专利标题

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US201762469073P| true| 2017-03-09|2017-03-09|

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PCT/US2018/021492|WO2018165395A1|2017-03-09|2018-03-08|Fused imidazo-piperidine jak inhibitors|

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